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Literature summary for 3.2.1.6 extracted from
- Cloning of a beta-1,3-1,4-glucanase gene from Bacillus subtilis MA139 and its functional expression in Escherichia coli (2009), Appl. Biochem. Biotechnol., 152, 334-342.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arginine | 8% activation at 10 mM | Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain Bl21 | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 97% inhibition at 10 mM | Bacillus subtilis | |
| EDTA | 28.5% inhibition at 10 mM | Bacillus subtilis | |
| Mn2+ | 54% inhibition at 10 mM | Bacillus subtilis | |
| Na2MoO4 | 20% inhibition at 10 mM | Bacillus subtilis | |
| Zn2+ | 7.6% inhibition at 10 mM | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 9% activation at 10 mM | Bacillus subtilis | |
| additional information | the enzyme activity is not affected by Mg2+, Ca2+, KI, and sucrose at 10 mM | Bacillus subtilis | |
| Na2SeO3 | 9% activation at 10 mM | Bacillus subtilis | |
| NaCl | 12.5% activation at 10 mM | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24440 | - |
x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
| 25500 | - |
x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | A8CGP1 | isolated from pig intestine | - |
| Bacillus subtilis MA139 | A8CGP1 | isolated from pig intestine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 379.1 | - |
purified recombinant His-tagged enzyme, substrate lichenan | Bacillus subtilis |
| 728.8 | - |
purified recombinant His-tagged enzyme, substrate beta-glucan from Hordeum vulgare | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-1,4-glucan + H2O | from Hordeum vulgare, preferred substrate | Bacillus subtilis | ? | - |
? | |
| beta-1,3-1,4-glucan + H2O | from Hordeum vulgare, preferred substrate | Bacillus subtilis MA139 | ? | - |
? | |
| lichenan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| lichenan + H2O | - |
Bacillus subtilis MA139 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Bacillus subtilis |
| More | the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | inactivation above 70°C, profile, overview | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, 80% remaining activity after 2 h | Bacillus subtilis |
| 50 | - |
purified recombinant His-tagged enzyme, 70% remaining activity after 2 h | Bacillus subtilis |
| 60 | - |
purified recombinant His-tagged enzyme, 20% remaining activity after 30 min, below 10% after 60 min | Bacillus subtilis |
| 70 | - |
purified recombinant His-tagged enzyme, inactivation within 10 min | Bacillus subtilis |
| 80 | - |
purified recombinant His-tagged enzyme, inactivation within 5 min | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1350 | - |
lichenan | pH 6.4, 40°C, substrate from Hordeum vulgare | Bacillus subtilis | |
| 7207 | - |
beta-1,3-1,4-glucan | pH 6.4, 40°C, purified recombinant His-tagged enzyme, substrate from Hordeum vulgare | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
recombinant enzyme | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | profile, overview | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified recombinant His-tagged enzyme, 40°C, 1 h, stable | Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | sequence calculation | - |
5.55 |
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