Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.6 extracted from

  • Murray, P.G.; Grassick, A.; Laffey, C.D.; Cuffe, M.M.; Higgins, T.; Savage, A.V.; Planas, A.; Tuohy, M.G.
    Isolation and characterization of a thermostable endo-beta-glucanase active on 1,3-1,4-beta-D-glucans from the aerobic fungus Talaromyces emersonii CBS 814.70 (2001), Enzyme Microb. Technol., 29, 90-98 .
    View publication on PubMed
Search for more literature for 3.2.1.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 lichenan Km value 13.38 mg/ml, pH 5.0, 50°C Rasamsonia emersonii
additional information
-
 Barley beta-glucan Km value 13.38 mg/ml, pH 5.0, 50°C Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rasamsonia emersonii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40700
-
 gel filtration Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
-
-
Rasamsonia emersonii CBS 814.70
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 77% carbohydrate content Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1652
-
 substrate lichenan, pH 5.0, 50°C Rasamsonia emersonii
1963
-
 substrate barley beta-glucan, pH 5.0, 50°C Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Rasamsonia emersonii ?
-
 ?
barley beta-glucan + H2O
-
 Rasamsonia emersonii CBS 814.70 ?
-
 ?
lichenan + H2O
-
 Rasamsonia emersonii ?
-
 ?
lichenan + H2O
-
 Rasamsonia emersonii CBS 814.70 ?
-
 ?
additional information enzyme is active on 1,3-1,4-beta-D-glucans, with no activity against a range of aryl and methylumbelliferyl-beta-glycosides, alpha- and beta-linked polysaccharides, including oat spelts xylan, rhodymenan, and polygalacturonic acid, avicel, laminarin or pachyman Rasamsonia emersonii ?
-
 ?
additional information enzyme is active on 1,3-1,4-beta-D-glucans, with no activity against a range of aryl and methylumbelliferyl-beta-glycosides, alpha- and beta-linked polysaccharides, including oat spelts xylan, rhodymenan, and polygalacturonic acid, avicel, laminarin or pachyman Rasamsonia emersonii CBS 814.70 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40700, SDS-PAGE Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 15 min, 15% residual activity Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
269
-
 Barley beta-glucan pH 5.0, 50°C Rasamsonia emersonii
485
-
 lichenan pH 5.0, 50°C Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Rasamsonia emersonii

pH Range

pH Minimum pH Maximum Comment Organism
2.5 7.4 more than 50% of maximum activity Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii isoelectric focusing
-
 4.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan kcat/Km value 20.1 ml/mg/s, pH 5.0, 50°C Rasamsonia emersonii
additional information
-
 lichenan kcat/Km value 36.2 ml/mg/s, pH 5.0, 50°C Rasamsonia emersonii