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Literature summary for 3.2.1.6 extracted from
- Gene cloning, heterologous expression and characterization of a Coprinopsis cinerea endo-beta-1,3(4)-glucanase (2017), Fungal Biol., 121, 61-68 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Coprinopsis cinerea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 5% of initial activity | Coprinopsis cinerea |  |
| EDTA | 1 mM, 84% of initial activity | Coprinopsis cinerea | |
| SDS | 1 mM, 72% of initial activity | Coprinopsis cinerea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Barley beta-glucan | Km value 20.8 mg/ml, pH 6.0, 50°C | Coprinopsis cinerea | |
| additional information | - |
Laminarin | Km value 6.5 mg/ml, pH 6.0, 50°C | Coprinopsis cinerea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 130% of initial activity | Coprinopsis cinerea | |
| Co2+ | 1 mM, 143% of initial activity | Coprinopsis cinerea | |
| Fe3+ | 1 mM, 116% of initial activity | Coprinopsis cinerea | |
| Mg2+ | 1 mM, 113% of initial activity | Coprinopsis cinerea | |
| Mn2+ | 1 mM, 215% of initial activity | Coprinopsis cinerea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | A8N2G5 | - |
- |
| Coprinopsis cinerea ATCC MYA-4618 | A8N2G5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Coprinopsis cinerea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | high expression during mycelial stage | Coprinopsis cinerea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.5 | - |
substrate laminarin, pH 6.0, 37°C | Coprinopsis cinerea |
| 39.8 | - |
substrate periodate-oxidized laminarin, pH 6.0, 37°C | Coprinopsis cinerea |
| 72.1 | - |
substrate carboxymethylpachyman, pH 6.0, 37°C | Coprinopsis cinerea |
| 118.8 | - |
substrate barley beta-glucan, pH 6.0, 37°C | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
| carboxymethylpachyman + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
| laminarin + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
| laminaritetraose + H2O | - |
Coprinopsis cinerea | 2 laminaribiose | - |
? | |
| additional information | enzyme only acts on substrates containing beta-1,3 glycosidic bonds but not on substrates containing only beta-1,4- or beta-1,6-glycosidic bonds | Coprinopsis cinerea | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CC1G_01665 | - |
Coprinopsis cinerea |
| ENG16A | - |
Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Coprinopsis cinerea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
20% of maximum activity | Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Coprinopsis cinerea |
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Release 2023.1 (January 2023)
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