Cloned (Comment) | Organism |
---|---|
lamR gene | Rhodothermus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant LamR, from transformed Escherichia coli cells | Rhodothermus marinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-1,4-beta-D-glucan + H2O | LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin and 1,3-1,4-beta-glucan from the cell walls of barley endosperm, analysis of reaction products | Rhodothermus marinus | ? | - |
? | |
1,3-beta-oligosaccharide + H2O | from DP3 to DP6 | Rhodothermus marinus | ? | - |
? | |
curdlan + H2O | from Alcaligenes faecalis, LamR cleaves the 1,3-beta-linkages of 3-O-substituted glucose units in 1,3-beta-glucans such as laminarin and curdlan | Rhodothermus marinus | ? | - |
? | |
laminarin + H2O | from Laminaria digitata, LamR cleaves the 1,3-beta-linkages of 3-O-substituted glucose units in 1,3-beta-glucans such as laminarin and curdlan, analysis of reaction products | Rhodothermus marinus | ? | - |
? | |
laminaritriose + H2O | - |
Rhodothermus marinus | laminaribiose + D-glucose | - |
? | |
lichenin + H2O | LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin, analysis of reaction products | Rhodothermus marinus | ? | - |
? | |
additional information | hydrolysis occurs with retention of the beta-configuration at the anomeric carbon, LamR performs transglycosylation to generate both 1,3-beta-glycosidic and 1,4-beta-glycosidic linkages, a laminaribiosyl unit is the minimum requirement for formation of an enzyme-substrate complex and subsequent hydrolysis/transglycosylation, no substrate: laminaribiose | Rhodothermus marinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LamR | - |
Rhodothermus marinus |
More | LamR belongs to glycosyl hydrolase family 16 | Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
88 | - |
- |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostable enzyme | Rhodothermus marinus |
80 | - |
2 days, stable | Rhodothermus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rhodothermus marinus |