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Literature summary for 3.2.1.6 extracted from

  • Nagasaki, S.; Mori, H.; Yamamoto, S.
    Characterization of endo beta-1,3-glucanase IV from Flavobacterium dorminator var. glucanolyticae FA-5 (1981), Agric. Biol. Chem., 45, 2689-2694.
No PubMed abstract available
Search for more literature for 3.2.1.6

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 72% inhibition of beta-1,3-glucanase activity after incubation with 1 mM at 5°C, pH 6.0, for 3 h Flavobacterium dormitator
Hg2+ 96% inhibition of beta-1,3-glucanase IV activity after incubation with 1 mM at 5°C, pH 6.0, for 3 h, no inhibition in presence of beta-mercaptoethanol Flavobacterium dormitator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31900 33000 gel filtration, SDS-PAGE, ultracentrifugation Flavobacterium dormitator

Organism

Organism UniProt Comment Textmining
Flavobacterium dormitator
-
 var. glucanolyticae FA-5
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 10.3% carbohydrate, 17 hexose units Flavobacterium dormitator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 laminarin as substrate Flavobacterium dormitator
0.46
-
 yeast glucan as substrate Flavobacterium dormitator
1.38
-
 pachyman as substrate Flavobacterium dormitator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O yeast glucan Flavobacterium dormitator laminaridextrins
-
 ?
curdlan + H2O paramylon Flavobacterium dormitator laminaripentaose main product ?
laminarin + H2O
-
 Flavobacterium dormitator D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose main product laminaripentaose ?
lichenin + H2O
-
 Flavobacterium dormitator beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers laminaripentaose main product ?
additional information transglycosylase activity Flavobacterium dormitator ?
-
 ?
pachyman + H2O
-
 Flavobacterium dormitator laminarisaccharides
-
 ?

Synonyms

Synonyms Comment Organism
endo beta-1,3-glucanase IV
-
 Flavobacterium dormitator
endo-1,3(4)-beta-D-glucanase
-
 Flavobacterium dormitator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 55 40°C with yeast glucan, 55°C with laminarin as substrate Flavobacterium dormitator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 enzyme IV: 80% of activity after heating at 50°C for 10 min Flavobacterium dormitator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 pH 6.0 with yeast glucan, pH 6.5 with laminarin as substrate Flavobacterium dormitator

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 enzymes I, II and IV: 10 min, 55°C, stable, unstable below pH 6 and above pH 9 Flavobacterium dormitator