Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.6 extracted from

  • Merzlov, D.A.; Zorov, I.N.; Dotsenko, G.S.; Denisenko, Y.A.; Rozhkova, A.M.; Satrutdinov, A.D.; Rubtsova, E.A.; Kondratieva, E.G.; Sinitsyn, A.P.
    Properties of enzyme preparations and homogeneous enzymes - endoglucanases EG2 Penicillium verruculosum and LAM Myceliophthora thermophila (2015), Biochemistry (Moscow), 80, 473-482 .
    View publication on PubMed
Search for more literature for 3.2.1.6

Application

Application Comment Organism
degradation oligosaccharides with degree of polymerization 2-10 are formed by hydrolysis of beta-glucan and laminarin. The recombinant enzyme preparations are fast and effective in decreasing the reduced viscosity of wholegrain barley extract than some commercial enzyme preparations Thermothelomyces heterothallicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Talaromyces verruculosus Thermothelomyces heterothallicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin Km value 1.1 mg/ml, pH 5.0, 50°C Thermothelomyces heterothallicus
additional information
-
 beta-Glucan Km value 2.5 mg/ml, pH 5.0, 50°C Thermothelomyces heterothallicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothelomyces heterothallicus
-
-

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3
-
 substrate curdlan, pH 5.0, 50°C Thermothelomyces heterothallicus
15
-
 substrate laminarin, pH 5.0, 50°C Thermothelomyces heterothallicus
54
-
 substrate beta-glucan, pH 5.0, 50°C Thermothelomyces heterothallicus
56
-
 substrate lichenan, pH 5.0, 50°C Thermothelomyces heterothallicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10 Thermothelomyces heterothallicus ?
-
 ?
curdlan + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
laminarin + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
lichenan + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
additional information no substrates: xylan, microcrystalline cellulose, and 4-nitrophenylbeta-D-glucopyranoside Thermothelomyces heterothallicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Thermothelomyces heterothallicus

Synonyms

Synonyms Comment Organism
LAM
-
 Thermothelomyces heterothallicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
59 67
-
 Thermothelomyces heterothallicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 70 more than 50% of maximum activity within Thermothelomyces heterothallicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
156
-
 Laminarin pH 5.0, 50°C Thermothelomyces heterothallicus
156
-
 beta-Glucan pH 5.0, 50°C Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3 5.2
-
 Thermothelomyces heterothallicus

pH Range

pH Minimum pH Maximum Comment Organism
3.8 6 more than 50% of maximum activity within Thermothelomyces heterothallicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 Laminarin kcat/Km value 10.6 ml/s/mg, pH 5.0, 50°C Thermothelomyces heterothallicus
additional information
-
 beta-Glucan kcat/Km value 19.8 ml/s/mg, pH 5.0, 50°C Thermothelomyces heterothallicus