Literature summary for 3.2.1.6 extracted from

Britto, D.S.; Pirovani, C.P.; Andrade, B.S.; Dos Santos, T.P.; Pungartnik, C.; Cascardo, J.C.; Micheli, F.; Gesteira, A.S.
Recombinant beta-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth (2013), Mol. Biol. Rep., 40, 5417-5427.

Search for more literature for 3.2.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant heterologous enzyme expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)	Theobroma cacao

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22000	-	x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE	Theobroma cacao
25580	-	x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE	Theobroma cacao

Organism

Organism	UniProt	Comment	Textmining
Theobroma cacao	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography	Theobroma cacao

Subunits

Subunits	Comment	Organism
?	x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE	Theobroma cacao
monomer	the enzyme is a single chain protein presenting 7 alpha-helices, 10 beta-sheets and 4 salt bridges	Theobroma cacao

Synonyms

Synonyms	Comment	Organism
beta-1,3-1,4-glucanase	-	Theobroma cacao
TcGlu2	-	Theobroma cacao

General Information

General Information	Comment	Organism
additional information	the enzyme has an active site of 13 amino acids typical of glucanase with beta-1,3 and 1,4 action mode, comparative molecular modelling, overview. The enzyme's active site is composed of 13 amino acids, Trp93, Leu94, Lys95, Asp96, His97, Gly98, Pro99, Asp100, Lys101, Gly102, Phe103, Glu104, Asp105, a space of 2831 A, and presents alpha-helices and loop structures	Theobroma cacao
physiological function	the enzyme causes hydrolysis of the membrane of hyphae from Moniliophthora perniciosa. Recombinant beta-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa strain ALF553 mycelial growth	Theobroma cacao

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Release 2023.1 (January 2023)