Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.6 extracted from

  • Nagata, S.; Sawatani, M.; Kuriyama, M.; Misono, H.; Nagasaki, S.
    Purification and characterization of nonlytic endo-beta-1,3-glucanase I from Flavobacterium dormitator var. glucanolyticae (1990), Agric. Biol. Chem., 54, 2107-2114.
No PubMed abstract available
Search for more literature for 3.2.1.6

General Stability

General Stability Organism
stable against proteolysis, full activity after papain treatment for 20 h Flavobacterium dormitator

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 87% of activity with 10 mM Flavobacterium dormitator
Hg2+ 99% inhibition with 10 mM Flavobacterium dormitator

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ 1.5fold activity in the presence of 10 mM NiSO4 Flavobacterium dormitator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 SDS-PAGE Flavobacterium dormitator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Flavobacterium dormitator use as carbon source ?
-
 ?

Organism

Organism UniProt Comment Textmining
Flavobacterium dormitator
-
 var. glucanolyticae FA-5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium dormitator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
 purified enzyme, laminarin as substrate Flavobacterium dormitator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O yeast glucan Flavobacterium dormitator laminaridextrins
-
 ?
curdlan + H2O
-
 Flavobacterium dormitator laminaripentaose
-
 ?
laminarin + H2O
-
 Flavobacterium dormitator D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose D-glucose + laminaribiose ?
laminarin + H2O
-
 Flavobacterium dormitator D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose laminaritriose + D-glucose ?
laminarin + H2O use as carbon source Flavobacterium dormitator ?
-
 ?
lichenin + H2O weak activity of enzyme I Flavobacterium dormitator beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
 ?
pachyman + H2O weak activity of enzyme I Flavobacterium dormitator laminarisaccharides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000 Flavobacterium dormitator

Synonyms

Synonyms Comment Organism
nonlytic endo-beta-1,3-glucanase I
-
 Flavobacterium dormitator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 55 55°C laminarin as substrate Flavobacterium dormitator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 enzyme I: full activity on heating at 55°C at pH 6.0 for 10 min, substantial loss of activity by heating at 70°C for 10 min Flavobacterium dormitator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Flavobacterium dormitator

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Flavobacterium dormitator