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Literature summary for 3.2.1.6 extracted from

  • Kim, D.; Lee, M.; Cho, H.; Lee, J.; Lee, M.; Chung, C.; Shin, D.; Rhee, Y.; Son, K.; Park, H.
    Genetic and functional characterization of an extracellular modular GH6 endo-beta-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13 (2016), Antonie van Leeuwenhoek, 109, 1-12 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cellulosimicrobium funkei

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, slight inhibition Cellulosimicrobium funkei
N-bromosuccinimide 10 min, 95% loss of activity Cellulosimicrobium funkei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.2fold increase in activity Cellulosimicrobium funkei
Co2+ 1 mM, 1.4fold increase in activity Cellulosimicrobium funkei
Mn2+ 1 mM, 1.2fold increase in activity Cellulosimicrobium funkei

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium funkei A0A0P0IZM9
-
-
Cellulosimicrobium funkei HY-13 A0A0P0IZM9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
substrate carboxymethylcellulose, pH 5.0, 50°C Cellulosimicrobium funkei
6.2
-
substrate barley beta-1,3-1,4-D-glucan, pH 5.0, 50°C Cellulosimicrobium funkei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Cellulosimicrobium funkei ?
-
?
avicel + H2O
-
Cellulosimicrobium funkei HY-13 ?
-
?
barley beta-1,3-1,4-D-glucan + H2O
-
Cellulosimicrobium funkei cellotriose
-
?
carboxymethylcellulose + H2O 45% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
?
carboxymethylcellulose + H2O 45% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei HY-13 ?
-
?
cellopentaose + H2O
-
Cellulosimicrobium funkei cellobiose + cellotriose
-
?
cellopentaose + H2O
-
Cellulosimicrobium funkei HY-13 cellobiose + cellotriose
-
?
konjac glucomannan + H2O 40% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
?
lichenan + H2O 48% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
?
lichenan + H2O 48% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei HY-13 ?
-
?
additional information activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan Cellulosimicrobium funkei ?
-
?
additional information activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan Cellulosimicrobium funkei HY-13 ?
-
?

Subunits

Subunits Comment Organism
? x * 56000, calculated and SDS-PAGE Cellulosimicrobium funkei

Synonyms

Synonyms Comment Organism
CelL
-
Cellulosimicrobium funkei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Cellulosimicrobium funkei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 12 min Cellulosimicrobium funkei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Cellulosimicrobium funkei

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 1 h, 90% residual activity Cellulosimicrobium funkei

pI Value

Organism Comment pI Value Maximum pI Value
Cellulosimicrobium funkei calculated
-
4.5