Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cellulosimicrobium funkei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, slight inhibition | Cellulosimicrobium funkei | |
N-bromosuccinimide | 10 min, 95% loss of activity | Cellulosimicrobium funkei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.2fold increase in activity | Cellulosimicrobium funkei | |
Co2+ | 1 mM, 1.4fold increase in activity | Cellulosimicrobium funkei | |
Mn2+ | 1 mM, 1.2fold increase in activity | Cellulosimicrobium funkei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulosimicrobium funkei | A0A0P0IZM9 | - |
- |
Cellulosimicrobium funkei HY-13 | A0A0P0IZM9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
substrate carboxymethylcellulose, pH 5.0, 50°C | Cellulosimicrobium funkei |
6.2 | - |
substrate barley beta-1,3-1,4-D-glucan, pH 5.0, 50°C | Cellulosimicrobium funkei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Cellulosimicrobium funkei | ? | - |
? | |
avicel + H2O | - |
Cellulosimicrobium funkei HY-13 | ? | - |
? | |
barley beta-1,3-1,4-D-glucan + H2O | - |
Cellulosimicrobium funkei | cellotriose | - |
? | |
carboxymethylcellulose + H2O | 45% of the activity with barley beta-1,3-1,4-D-glucan | Cellulosimicrobium funkei | ? | - |
? | |
carboxymethylcellulose + H2O | 45% of the activity with barley beta-1,3-1,4-D-glucan | Cellulosimicrobium funkei HY-13 | ? | - |
? | |
cellopentaose + H2O | - |
Cellulosimicrobium funkei | cellobiose + cellotriose | - |
? | |
cellopentaose + H2O | - |
Cellulosimicrobium funkei HY-13 | cellobiose + cellotriose | - |
? | |
konjac glucomannan + H2O | 40% of the activity with barley beta-1,3-1,4-D-glucan | Cellulosimicrobium funkei | ? | - |
? | |
lichenan + H2O | 48% of the activity with barley beta-1,3-1,4-D-glucan | Cellulosimicrobium funkei | ? | - |
? | |
lichenan + H2O | 48% of the activity with barley beta-1,3-1,4-D-glucan | Cellulosimicrobium funkei HY-13 | ? | - |
? | |
additional information | activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan | Cellulosimicrobium funkei | ? | - |
? | |
additional information | activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan | Cellulosimicrobium funkei HY-13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, calculated and SDS-PAGE | Cellulosimicrobium funkei |
Synonyms | Comment | Organism |
---|---|---|
CelL | - |
Cellulosimicrobium funkei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cellulosimicrobium funkei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 12 min | Cellulosimicrobium funkei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Cellulosimicrobium funkei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 1 h, 90% residual activity | Cellulosimicrobium funkei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cellulosimicrobium funkei | calculated | - |
4.5 |