Information on EC 6.2.1.3 - long-chain-fatty-acid-CoA ligase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
6.2.1.3
-
RECOMMENDED NAME
GeneOntology No.
long-chain-fatty-acid-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
-
-
-
-
Phosphorylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
6-gingerol analog biosynthesis (engineered)
-
-
alkane biosynthesis II
-
-
arachidonate biosynthesis
-
-
ceramide biosynthesis
-
-
cutin biosynthesis
-
-
fatty acid alpha-oxidation II
-
-
fatty acid alpha-oxidation III
-
-
fatty acid beta-oxidation (peroxisome, yeast)
-
-
fatty acid beta-oxidation I
-
-
fatty acid beta-oxidation II (peroxisome)
-
-
fatty acid beta-oxidation VI (peroxisome)
-
-
Fatty acid biosynthesis
-
-
Fatty acid degradation
-
-
fatty acid salvage
-
-
gamma-linolenate biosynthesis II (animals)
-
-
icosapentaenoate biosynthesis II (6-desaturase, mammals)
-
-
icosapentaenoate biosynthesis III (8-desaturase, mammals)
-
-
linoleate biosynthesis II (animals)
-
-
lipid metabolism
-
-
long chain fatty acid ester synthesis (engineered)
-
-
long-chain fatty acid activation
-
-
Metabolic pathways
-
-
octane oxidation
oleate biosynthesis I (plants)
-
-
palmitate biosynthesis II (bacteria and plants)
-
-
phosphatidylcholine acyl editing
-
-
phytol degradation
-
-
sporopollenin precursors biosynthesis
-
-
stearate biosynthesis I (animals and fungi)
-
-
stearate biosynthesis II (bacteria and plants)
-
-
suberin monomers biosynthesis
-
-
wax esters biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
long-chain fatty acid:CoA ligase (AMP-forming)
Acts on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. The liver enzyme acts on acids from C6 to C20; that from brain shows high activity up to C24.
CAS REGISTRY NUMBER
COMMENTARY hide
9013-18-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
gene faaB or AN8280.3 encoding isozyme FaaB
-
-
Manually annotated by BRENDA team
-
TrEMBL
Manually annotated by BRENDA team
isozyme ACS-3, gene acs-3
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 1S-4
UniProt
Manually annotated by BRENDA team
strain 1S-4
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain B23, gene acsA
UniProt
Manually annotated by BRENDA team
strain B23, gene acsA
UniProt
Manually annotated by BRENDA team
Iberian Guadyerbas x Landrace cross breed
-
-
Manually annotated by BRENDA team
strain HB8
-
-
Manually annotated by BRENDA team
red flour beetle
-
-
Manually annotated by BRENDA team
strain 427
-
-
Manually annotated by BRENDA team
Ulmus sp.
elm
-
-
Manually annotated by BRENDA team
2 enzyme forms: long-chain acyl-CoA synthetase I and long-chain acyl-CoA synthetase II
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arachidonic acid + ATP + CoA
arachidonoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
?
ATP + (11Z)-eicosenoate + CoA
AMP + diphosphate + (11Z)-eicosenoyl-CoA
show the reaction diagram
-
-
?
ATP + (15Z)-tetracosenoate + CoA
AMP + diphosphate + (15Z)-tetracosenoyl-CoA
show the reaction diagram
-
-
?
ATP + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + CoA
AMP + diphosphate + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA
show the reaction diagram
-
-
?
ATP + (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
AMP + diphosphate + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA
show the reaction diagram
-
-
?
ATP + (6Z)-octadecenoate + CoA
AMP + diphosphate + (6Z)-octadecenoyl-CoA
show the reaction diagram
-
-
?
ATP + (6Z,12Z, 15Z)-octadecatrienoate + CoA
AMP + diphosphate + (6Z,12Z,15Z)-octadecatrienoyl-CoA
show the reaction diagram
-
-
?
ATP + (6Z,9Z,12Z)-octadecatrienoate + CoA
AMP + diphosphate + (6Z,9Z,12Z)-octadecatrienoyl-CoA
show the reaction diagram
-
-
?
ATP + (9E)-octadecenoate + CoA
AMP + diphosphate + (9E)-octadecenoyl-CoA
show the reaction diagram
-
-
?
ATP + (9Z)-hexadecenoate + CoA
AMP + diphosphate + (9Z)-hexadecenoyl-CoA
show the reaction diagram
ATP + (9Z)-octadecenoate + CoA
AMP + diphosphate + (9Z)-octadecenoyl-CoA
show the reaction diagram
-
-
?
ATP + (9Z)-tetradecenoate + CoA
AMP + diphosphate + (9Z)-tetradecenoyl-CoA
show the reaction diagram
-
-
?
ATP + (9Z,12Z)-octadecadienoate + CoA
AMP + diphosphate + (9Z,12Z)-octadecadienoyl-CoA
show the reaction diagram
ATP + (9Z,12Z,15Z)-octadecatrienoate + CoA
AMP + diphosphate + (9Z,12Z,15Z)-octadecatrienoyl-CoA
show the reaction diagram
ATP + (R)-ibuprofen + CoA
AMP + diphosphate + (R)-ibuprofenoyl-CoA
show the reaction diagram
ATP + 1,12-dodecanedioic acid + CoA
AMP + diphosphate + ?
show the reaction diagram
-
-
-
-
ATP + 12-oxo-phytodienoic acid + CoA
AMP + diphosphate + 12-oxo-phytodienoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + 16-hydroxypalmitic acid + CoA
AMP + diphosphate + 16-hydroxypalmitoyl-CoA
show the reaction diagram
-
isoenzyme LACS2
-
-
?
ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA
AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA
AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA
show the reaction diagram
-
preferred substrate
-
-
?
ATP + a long-chain fatty acid + CoA
AMP + diphosphate + a long-chain fatty acyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + acetate + CoA
AMP + diphosphate + acetyl-CoA
show the reaction diagram
ATP + acylate + CoA
AMP + diphosphate + acyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + arachidoate + CoA
AMP + diphosphate + arachidoyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 40% activity and isoform Facl2 exhibits about 60% activity
-
-
?
ATP + arachidonate + CoA
AMP + diphosphate + arachidonoyl-CoA
show the reaction diagram
ATP + behenoate + CoA
AMP + diphosphate + behenoyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 65% activity
-
-
?
ATP + Bodipy 500/510 C4-C9 + CoA
?
show the reaction diagram
-
-
-
-
?
ATP + Bodipy-C16 + CoA
?
show the reaction diagram
-
-
-
-
?
ATP + butyrate + CoA
AMP + diphosphate + butyryl-CoA
show the reaction diagram
ATP + C1-Bodipy-C12 + CoA
?
show the reaction diagram
-
-
-
-
?
ATP + caproate + CoA
AMP + diphosphate + caproyl-CoA
show the reaction diagram
-
most preferred substrate for isoform Facl1 (100% activity)
-
-
?
ATP + capryloate + CoA
AMP + diphosphate + capryloyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 50% activity and isoform Facl2 exhibits about 45% activity
-
-
?
ATP + decanoate + CoA
AMP + diphosphate + decanoyl-CoA
show the reaction diagram
ATP + decenoate + CoA
AMP + diphosphate + decenoyl-CoA
show the reaction diagram
-
-
-
?
ATP + dinor-12-oxo-phytodienoic acid + CoA
AMP + diphosphate + dinor-12-oxo-phytodienoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + docosahexaenoate + CoA
AMP + diphosphate + docosahexaenoyl-CoA
show the reaction diagram
-
-
-
?
ATP + docosahexaneoate + CoA
AMP + diphosphate + docosahexaenoyl-CoA
show the reaction diagram
ATP + docosenoate + CoA
AMP + diphosphate + docosenoyl-CoA
show the reaction diagram
-
-
-
?
ATP + dodecanoate + CoA
AMP + diphosphate + dodecanoyl-CoA
show the reaction diagram
ATP + eicosapentaenoate + CoA
AMP + diphosphate + eicosapentaenoyl-CoA
show the reaction diagram
ATP + elaidate + CoA
AMP + diphosphate + elaiodyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + fatty acid + 4'-phosphopantetheine
AMP + diphosphate + acyl-4'-phosphopantetheine
show the reaction diagram
ATP + fatty acid + dephospho-CoA
AMP + diphosphate + acyl-dephospho-CoA
show the reaction diagram
ATP + fatty acid + N6-etheno-CoA
AMP + diphosphate + acyl-N6-etheno-CoA
show the reaction diagram
-
-
-
-
-
ATP + fatty acid + pantetheine
AMP + diphosphate + acyl-pantetheine
show the reaction diagram
ATP + heptadecanoate + CoA
AMP + diphosphate + heptadecanoyl-CoA
show the reaction diagram
ATP + hexacosanoate + CoA
AMP + diphosphate + hexacosanoyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 8% activity and isoform Facl2 exhibits about 23% activity
-
-
?
ATP + hexadecanoate + CoA
AMP + diphosphate + hexadecanoyl-CoA
show the reaction diagram
ATP + hexanoate + CoA
AMP + diphosphate + hexanoyl-CoA
show the reaction diagram
ATP + jasmonic acid + CoA
AMP + diphosphate + jasmonyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + laurate + CoA
AMP + diphosphate + lauroyl-CoA
show the reaction diagram
ATP + laurate + CoA
AMP + diphosphate + lauryl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 90% activity and isoform Facl2 exhibits about 55% activity
-
-
?
ATP + lignocerate + CoA
AMP + diphosphate + lignoceroyl-CoA
show the reaction diagram
ATP + lignocerate + CoA
AMP + diphosphate + lignoceryl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 30% activity
-
-
?
ATP + linoleate + CoA
AMP + diphosphate + lineoyl-CoA
show the reaction diagram
ATP + linoleate + CoA
AMP + diphosphate + linoleoyl-CoA
show the reaction diagram
ATP + linoleic acid + CoA
AMP + diphosphate + linoleoyl-CoA
show the reaction diagram
-
-
-
?
ATP + linolenate + CoA
AMP + diphosphate + linolenoyl-CoA
show the reaction diagram
ATP + long-chain carboxylic acid + CoA
?
show the reaction diagram
ATP + long-chain carboxylic acid + CoA
AMP + diphosphate + long-chain acyl-CoA
show the reaction diagram
ATP + melissoate + CoA
AMP + diphosphate + melissoyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 3% activity and isoform Facl2 exhibits about 1% activity
-
-
?
ATP + myristate + CoA
AMP + diphosphate + myristoyl-CoA
show the reaction diagram
ATP + myristate + CoA
AMP + diphosphate + myristyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 65% activity and isoform Facl2 exhibits about 85% activity
-
-
?
ATP + myristic acid + CoA
AMP + diphosphate + myristoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + n-tetracosanoic acid + CoA
AMP + diphosphate + n-tetracosanoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + nonanoate + CoA
AMP + diphosphate + nonanoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + o-succinyl-1-benzoate + CoA
AMP + diphosphate + o-succinyl-1-benzoyl-CoA
show the reaction diagram
ATP + octacosanoate + CoA
AMP + diphosphate + octacosanoyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 5% activity and isoform Facl2 exhibits about 3% activity
-
-
?
ATP + octadecanoate + CoA
AMP + diphosphate + octadecanoyl-CoA
show the reaction diagram
ATP + octanoate + CoA
AMP + diphosphate + octanoyl-CoA
show the reaction diagram
ATP + oleate + CoA
AMP + diphosphate + oleoyl-CoA
show the reaction diagram
ATP + oleic acid + CoA
AMP + diphosphate + oleoyl-CoA
show the reaction diagram
-
-
-
?
ATP + palmitate + CoA
AMP + diphosphate + palmitoyl-CoA
show the reaction diagram
ATP + palmitoleate + CoA
AMP + diphosphate + palmitoleoyl-CoA
show the reaction diagram
ATP + pentadecanoate + CoA
AMP + diphosphate + pentadecanoyl-CoA
show the reaction diagram
ATP + pristanic acid + CoA
AMP + diphosphate + pristanoyl-CoA
show the reaction diagram
-
-
-
-
ATP + stearate + CoA
AMP + diphosphate + stearoyl-CoA
show the reaction diagram
ATP + stearate + CoA
AMP + diphosphate + stearyl-CoA
show the reaction diagram
-
isoform Facl1 exhibits about 45% activity and isoform Facl2 exhibits about 75% activity
-
-
?
ATP + tetradecanoate + CoA
AMP + diphosphate + tetradecanoyl-CoA
show the reaction diagram
ATP + tridecanoate + CoA
AMP + diphosphate + tridecanoyl-CoA
show the reaction diagram
ATP + undecanoate + CoA
AMP + diphosphate + undecanoyl-CoA
show the reaction diagram
dATP + fatty acid + CoA
dAMP + diphosphate + acyl-CoA
show the reaction diagram
decanoic acid + ATP + CoA
decanoyl-CoA + AMP + diphosphate
show the reaction diagram
lauric acid
?
show the reaction diagram
-
-
-
-
?
lauric acid + ATP + CoA
lauroyl-CoA + AMP + diphosphate
show the reaction diagram
linoleic acid + ATP + CoA
linoleoyl-CoA + AMP + diphosphate
show the reaction diagram
linolenic acid + ATP + CoA
linolenoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
myristic acid + ATP + CoA
myristoyl-CoA + AMP + diphosphate
show the reaction diagram
oleic acid + ATP + CoA
oleoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-ibuprofen + CoA
AMP + diphosphate + (R)-ibuprofenoyl-CoA
show the reaction diagram
-
(R)-ibuprofenoyl-CoA synthetase and long-chain acyl-CoA synthetase are identical enzymes that are involved in the metabolism of various xenobiotics
-
?
ATP + a long-chain fatty acid + CoA
AMP + diphosphate + a long-chain fatty acyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + acylate + CoA
AMP + diphosphate + acyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + arachidonate + CoA
AMP + diphosphate + arachidonoyl-CoA
show the reaction diagram
ATP + hexadecanoate + CoA
AMP + diphosphate + hexadecanoyl-CoA
show the reaction diagram
ATP + long-chain carboxylic acid + CoA
?
show the reaction diagram
ATP + o-succinyl-1-benzoate + CoA
AMP + diphosphate + o-succinyl-1-benzoyl-CoA
show the reaction diagram
-
a step in the bacterial biosynthesis of menaquinone from chorismate, pathway overview
-
-
?
ATP + octadecanoate + CoA
AMP + diphosphate + octadecanoyl-CoA
show the reaction diagram
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
isoform Facl1 shows 156% activity and isoform Facl2 shows 193% activity at 1 mM
Co2+
-
isoform Facl2 shows 191% activity at 1 mM
Mn2+
-
divalent cation required, Mn2+ can replace Mg2+ in activation
Salts
-
at low concentrations all salts, including the chaotropic salts, e.g. NaNO3, NaClO4 and KSCN, and thelyotropic salts, e.g. (NH4)2SO4 and potassium phosphate are equally effective in activation. At higher concentrations the chaotropic salts inhibit the enzyme activity. Activity can be recovered by dilution
additional information
-
K+, NH4+ and Na+ show no effects on the enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid
-
competitive inhibitor of palmitoleic acid activation
2-Bromopalmitate
-
inhibition can be overcome by addition of phospholipid vesicles
2-Bromopalmitoyl-CoA
-
inhibition can be overcome by addition of phospholipid vesicles
5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]-5-oxopent-1-en-1-yl]sulfonyl)amino]adenosine
-
mechanism of covalent inhibition
5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]hexa-1,5-dien-1-yl]sulfonyl)amino]adenosine
-
mechanism of covalent inhibition
5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)amino]adenosine
-
mechanism of covalent inhibition
5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)amino]adenosine
-
mechanism of covalent inhibition
5'-O-([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)adenosine
-
mechanism of covalent inhibition
5'-O-([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)adenosine
-
mechanism of covalent inhibition
Ag+
-
isoform Facl1 shows 64% residual activity and isoform Facl2 shows 71% residual activity at 1 mM
arachidonate
arachidonic acid
-
concentration above 10 microM
Brij 58
-
maximal inhibition at 4% detergent
cis-9,10-methylene octadecanoic acid
-
IC50: 0.025 mM for ACS1-Flag fusion protein, 0.03-0.04 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein
Co2+
-
isoform Facl1 shows 50% residual activity at 1 mM
Cu2+
-
isoform Facl1 shows 64% residual activity and isoform Facl2 shows 33% residual activity at 1 mM
diphosphate
docosahexaenoate
-
unlabeled, inhibition of docosahexaenoate activation
EDTA
-
isoform Facl1 shows 12% residual activity and isoform Facl2 shows 26% residual activity at 1 mM
Eicosa-11,14,17-trienoic acid
-
competitive inhibitor of palmitoleic acid activation
eicosa-8,11,14-trienoic acid
-
competitive inhibitor of palmitoleic acid activation
Eicosa-8,14-dienoic acid
-
competitive inhibitor of palmitoleic acid activation
eicosapentanoate
-
inhibition of palmitoyl-CoA synthesis
-
fatty acids
Fe2+
-
isoform Facl1 shows 57% residual activity and isoform Facl2 shows 36% residual activity at 1 mM
GW1929
-
IC50: above 0.05 mM for ACS1-Flag fusion protein, 0.05 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein
Ketoprofen
-
non-competitive inhibition of the high affinity isoform
linoleate
-
inhibition of palmitoyl-CoA synthesis
linoleic acid
-
concentration above 10 microM
linolenate
-
inhibition of palmitoyl-CoA synthesis
Mn2+
-
isoform Facl1 shows 28% residual activity and isoform Facl2 shows 40% residual activity at 1 mM
naproxen
-
non-competitive inhibition of the high affinity isoform
Ni2+
-
isoform Facl1 shows 1.3% residual activity and isoform Facl2 shows no activity at 1 mM
oleate
-
inhibition of palmitoyl-CoA synthesis
oleic acid
-
concentration above 10 microM
oleoyl-CoA
-
inhibition of oleoyl-CoA synthesis
palmitate
-
linolenic acid activation
Perfluorodecanoic acid
-
no inhibition by short-chain perfluorinated fatty acids
Perfluorononanoic acid
-
no inhibition by short-chain perfluorinated fatty acids
Perfluorooctanoic acid
-
no inhibition by short-chain perfluorinated fatty acids
pioglitazone
-
IC50: 0.0015 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein
R-Fenoprofen
-
mixed inhibition of the high affinity isoform
R-Ibuprofen
-
mixed inhibition of the high affinity isoform
rosiglitazone
-
IC50: 0.0005 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein
Salts
-
at higher concentrations the chaotropic salts inhibit the enzyme activity, activity can be recovered by dilution. At low concentrations all salts, including the chaotropic salts and thelyotropic salts are equally effective in activation
-
Sodium cholate
-
maximal inhibition at 1% detergent
Sodium deoxycholate
-
-
thiazolidinediones
triacsin
-
competitive inhibitor of both ACS1 and ACS4
Triacsin A
Triacsin C
Triacsins
-
hierarchy of inhibitory potency in decreasing order: triacsin C, triacsin A, triacsin D/ triacsin B
Triton X-100
troglitazone
Tween 80
-
maximal inhibition at 4% detergent
Zn2+
-
isoform Facl1 shows 13% residual activity and isoform Facl2 shows 0.36% residual activity at 1 mM
Zwittergent 3-12
-
inhibition below the critical micellar concentration, 0.12%. below this concentrations no inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-synuclein
-
0.0063-0.634 mM
-
phosphatidylcholine
-
absolute requirement, acyl-CoA synthetase II
phosphatidylethanolamine
-
slight stimulation, acyl-CoA synthetase II
phosphatidylserine
-
slight stimulation, acyl-CoA synthetase II
Triacsin C
-
ACSL activity is 17fold higher in the presence of 0.02 mM triacsin C
Triton X-100
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167
(11Z)-eicosenoate
pH 8.5, 30C
0.253
(15Z)-tetracosenoate
pH 8.5, 30C
0.222
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate
pH 8.5, 30C
0.017
(5Z,8Z,11Z,14Z)-eicosatetraenoate
pH 8.5, 30C
0.048
(6Z)-octadecenoate
pH 8.5, 30C
0.02
(6Z,12Z, 15Z)-octadecatrienoate
pH 8.5, 30C
0.009
(6Z,9Z,12Z)-octadecatrienoate
pH 8.5, 30C
0.109
(9E)-octadecenoate
pH 8.5, 30C
0.009
(9Z)-hexadecenoate
pH 8.5, 30C
0.024
(9Z)-octadecenoate
pH 8.5, 30C
0.009
(9Z)-tetradecenoate
pH 8.5, 30C
0.021
(9Z,12Z)-octadecadienoate
pH 8.5, 30C
0.0314
16-hydroxypalmitic acid
-
isoenzyme LACS2
0.0375
18:3(n-3) fatty acid
-
-
-
0.06
20:3(n-6) fatty acid
-
-
-
0.41 - 3.3
4'-phosphopantetheine
0.0065 - 9.7
arachidonate
0.025 - 12.21
ATP
0.0064
caproate
-
isoform Facl1, at pH 7.5 and 60C
0.00053 - 4.7
CoA
1.93
coenzyme A
-
oleate activation
12.5 - 13.3
dATP
-
-
0.004 - 0.0083
Decanoate
0.11 - 0.29
dephospho-CoA
0.1
dodecanoate
pH 8.5, 30C
0.0155
eicosapentaenoate
-
-
0.0102
heptadecanoate
-
-
0.026
Heptanoate
-
-
0.014
hexadecanoate
pH 8.5, 30C
0.0112
hexanoate
-
-
0.00051 - 0.002
laurate
0.00168 - 0.0163
lauric acid
0.00222 - 0.041
linoleate
0.00188
linoleic acid
-
-
0.00164 - 0.0073
linolenate
0.0002 - 0.0024
myristate
0.0048
n-tetracosanoic acid
-
-
0.061 - 0.072
octadecanoate
0.00072 - 0.0408
Octanoate
0.00139 - 3
oleate
0.101
oleic acid
-
-
0.00021 - 6
palmitate
0.0015 - 0.00204
palmitoleate
1.1 - 2.5
pantetheine
0.0022
Pentadecanoate
-
-
0.00017 - 0.12
stearate
0.01
tetradecanoate
pH 8.5, 30C
0.0034
tridecanoate
-
-
0.0013
Undecanoate
-
-
additional information
additional information