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Literature summary for 6.2.1.3 extracted from
- Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions (2010), Cell. Mol. Life Sci., 67, 387-404.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C343S | no luminescence enhancement | Agrypnus binodulus |
| C343S/G344T | no luminescence enhancement | Agrypnus binodulus |
| C343S/G344T/L345S | increase of luminescence activity to 100% | Agrypnus binodulus |
| C343S/L345S | increase of luminescence activity | Agrypnus binodulus |
| G344T | no luminescence enhancement | Agrypnus binodulus |
| G344T/L345S | increase of luminescence activity to 27% | Agrypnus binodulus |
| L216R | no luminescence enhancement | Agrypnus binodulus |
| L345S | enhancement of luminescence | Agrypnus binodulus |
| additional information | chimeric protein with firefly luciferase, EC 1.13.12.7, 4% of luminescence activity from Photinus pyralis luciferase activity | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arachidonic acid | concentration above 10 microM | Drosophila melanogaster |  |
| linoleic acid | concentration above 10 microM | Drosophila melanogaster | |
| oleic acid | concentration above 10 microM | Drosophila melanogaster | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00168 | - |
lauric acid | - |
Drosophila melanogaster | |
| 0.00188 | - |
linoleic acid | - |
Drosophila melanogaster | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrypnus binodulus | - |
- |
- |
| Drosophila melanogaster | Q9VCC6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lauric acid | - |
Drosophila melanogaster | ? | - |
? | |
| linoleic acid + ATP + CoA | - |
Drosophila melanogaster | linoleoyl-CoA + AMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Abll | - |
Agrypnus binodulus |
| long fatty acyl-CoA synthetase | - |
Drosophila melanogaster |
| luciferase-like gene | - |
Agrypnus binodulus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Drosophila melanogaster |
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Release 2023.1 (January 2023)
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