General Stability | Organism |
---|---|
100 mM potassium phosphate stabilizes at 4°C | Escherichia coli |
ATP, stabilizes at 4EC | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
salts | at higher concentrations the chaotropic salts inhibit the enzyme activity, activity can be recovered by dilution. At low concentrations all salts, including the chaotropic salts and thelyotropic salts are equally effective in activation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
salts | at low concentrations all salts, including the chaotropic salts, e.g. NaNO3, NaClO4 and KSCN, and thelyotropic salts, e.g. (NH4)2SO4 and potassium phosphate are equally effective in activation. At higher concentrations the chaotropic salts inhibit the enzyme activity. Activity can be recovered by dilution | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | 47000 | gel filtration | Escherichia coli |
50000 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.103 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 10 mM potassium phosphate, 12 h, 70% loss of activity. 100 mM potassium phosphate or ATP stabilizes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + long-chain carboxylic acid + CoA | trans-DELTA9-18:1 fatty acid | Escherichia coli | AMP + diphosphate + long-chain acyl-CoA | - |
? | |
ATP + long-chain carboxylic acid + CoA | trans-DELTA9-16:1 fatty acid | Escherichia coli | AMP + diphosphate + long-chain acyl-CoA | - |
? |