Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Rattus norvegicus | |
ATP | above 15 mM | Rattus norvegicus | |
Brij 58 | maximal inhibition at 4% detergent | Rattus norvegicus | |
diphosphate | - |
Rattus norvegicus | |
sodium cholate | maximal inhibition at 1% detergent | Rattus norvegicus | |
Triton X-100 | maximal inhibition at 4% detergent | Rattus norvegicus | |
Tween 80 | maximal inhibition at 4% detergent | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | CoA | Rattus norvegicus | |
4.1 | - |
ATP | at 20 mM MgCl2 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus | |
Mg2+ | maximal activity at 15-20 mM MgCl2, no inhibition at higher concentrations | Rattus norvegicus | |
Mn2+ | divalent cation required, Mn2+ can replace Mg2+ in activation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a long-chain fatty acid + CoA = AMP + diphosphate + a long-chain fatty acyl-CoA | mitochondrial long chain fatty acyl-CoA ligase, unlike short chain ligases and medium chain ligases, does not utilize an adenylate as an intermediate in the formation of fatty acyl-CoA | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.3 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.8 | pH 6: about 50% of maximal activity, pH 8.9: about 60% of maximal activity | Rattus norvegicus |