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Literature summary for 6.2.1.3 extracted from

  • Kienow, L.; Schneider, K.; Bartsch, M.; Stuible, H.P.; Weng, H.; Miersch, O.; Wasternack, C.; Kombrink, E.
    Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana (2008), J. Exp. Bot., 59, 403-419.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is inducible by wounding Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of opcl1 mutant, At1g20510-KO-1, plants, which show no induction of jasmonate by wounding in contrast to the wild-type plants, jasmonate profiles in leaves, overview Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information subcellular localization study Arabidopsis thaliana
-
-
peroxisome
-
Arabidopsis thaliana 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
Col-0, Ws-0, gene OPCL1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression levels of OPCL1 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 12-oxo-phytodienoic acid + CoA
-
Arabidopsis thaliana AMP + diphosphate + 12-oxo-phytodienoyl-CoA
-
?
ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA
-
Arabidopsis thaliana AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA
-
?
ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA preferred substrate Arabidopsis thaliana AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA
-
?
ATP + dinor-12-oxo-phytodienoic acid + CoA
-
Arabidopsis thaliana AMP + diphosphate + dinor-12-oxo-phytodienoyl-CoA
-
?
ATP + jasmonic acid + CoA
-
Arabidopsis thaliana AMP + diphosphate + jasmonyl-CoA
-
?
ATP + myristic acid + CoA
-
Arabidopsis thaliana AMP + diphosphate + myristoyl-CoA
-
?
additional information the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes Arabidopsis thaliana ?
-
?
additional information enzyme family members display activity towards different biosynthetic precursors of jasmonic acid, including 12-oxo-phytodienoic acid, i.e. OPDA, dinor-OPDA, 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid, i.e. OPC-8, and OPC-6, substrate specificity of protein At1g20510 readily converting OPDA, dnOPDA, OPC-8, OPC-6, and myristic acid, OPC-8 is the preferred substrate, overview Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
fatty acyl-CoA synthetase
-
Arabidopsis thaliana
More the enzyme belongs to a fatty acyl-CoA synthetase family, which is distinct from the known long-chain fatty acyl-CoA synthetase, LACS, and the 4-coumarate:CoA ligase, 4CL, families, overview Arabidopsis thaliana
OPC-8:CoA ligase
-
Arabidopsis thaliana
OPCL1
-
Arabidopsis thaliana
protein At1g20510
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana