Any feedback?
Literature summary for 6.2.1.3 extracted from
- Long-chain acyl-coenzyme A synthetase from rat liver brain microsomes. Kinetic studies using [1-14C]docosahexaenoic acid substrate (1984), Eur. J. Biochem., 145, 21-29.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | enhances activity | Rattus norvegicus |  |
General Stability
| General Stability | Organism |
|---|---|
| 36% loss of activity after freezing and thawing | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arachidonate | - |
Rattus norvegicus | |
| docosahexaenoate | unlabeled, inhibition of docosahexaenoate activation | Rattus norvegicus | |
| fatty acids | saturated fatty acids do not inhibit activation of docosahexaenoate or palmitate. Unsaturated fatty acids, except nervonic acid, inhibit the activation of docosahexaenoate acid. Moderate inhibition by oleate, linoleate, eicosapentanoate, and palmitate | Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| Pb2+ | - |
Rattus norvegicus | |
| SDS | - |
Rattus norvegicus | |
| sodium deoxycholate | - |
Rattus norvegicus | |
| Triton X-100 | slight inhibition when incubated with microsomes at 0°C for 30 min | Rattus norvegicus | |
| Zwittergent 3-12 | inhibition below the critical micellar concentration, 0.12%. below this concentrations no inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00488 | - |
CoA | - |
Rattus norvegicus | |
| 0.185 | - |
ATP | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | Km: 0.555 mM | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + docosahexaenoate + CoA | - |
Rattus norvegicus | AMP + diphosphate + docosahexaenoyl-CoA | - |
? | |
| ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life is 26.5 min | Rattus norvegicus |
| 45 | - |
half-life: 3.3 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
