Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
G401L | inactive enzyme | Rattus norvegicus |
L399M | no significant alteration of activity compared to the wild type enzyme | Rattus norvegicus |
Q525K | reduced activity | Rattus norvegicus |
S291Y | mutant prefers 20:5 and 22:6 substrates and has an increased Km for ATP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
CoA | mutant S291Y, at 37°C, in 175 mM Tris-HCl, at pH 7.4 | Rattus norvegicus | |
0.0067 | - |
CoA | wild type enzyme, at 37°C, in 175 mM Tris-HCl, at pH 7.4 | Rattus norvegicus | |
0.0068 | - |
CoA | mutant Q525K, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.0088 | - |
CoA | mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.02 | - |
palmitate | mutant Q525K, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.0211 | - |
palmitate | mutant S291Y, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.0238 | - |
palmitate | mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.0281 | - |
palmitate | wild type enzyme, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.087 | - |
ATP | wild type enzyme, at 37°C, in 175 mM Tris-HCl, at pH 7.4 | Rattus norvegicus | |
0.125 | - |
ATP | mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 | Rattus norvegicus | |
0.242 | - |
ATP | mutant Q525K, at 37°C, in 175 mM Tris-HCl, at pH 7.4 | Rattus norvegicus | |
0.502 | - |
ATP | mutant S291Y, at 37°C, in 175 mM Tris-HCl, at pH 7.4 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | O35547 | - |
- |
Purification (Comment) | Organism |
---|---|
M2-anti-FLAG agarose column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + arachidonate + CoA | the activity with arachidonate is twice as high as with palmitate | Rattus norvegicus | AMP + diphosphate + arachidonoyl-CoA | - |
? | |
ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACSL4 | prefers longer chain polyunsaturated fatty acids as substrates | Rattus norvegicus |
long chain acyl-CoA synthetase 4 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |