Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fatty acids | palmitoleate, oleate and linoleate are competitive inhibitors of the activation of each other | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | 0.028 | CoA | - |
Rattus norvegicus | |
0.23 | 0.29 | dephospho-CoA | - |
Rattus norvegicus | |
0.41 | 0.43 | 4'-phosphopantetheine | - |
Rattus norvegicus | |
1.1 | 1.2 | pantetheine | - |
Rattus norvegicus | |
2.3 | 2.4 | ATP | - |
Rattus norvegicus | |
12.5 | 13.3 | dATP | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.2 | - |
mitochondrial enzyme | Rattus norvegicus |
28.7 | - |
microsomal enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 4 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + fatty acid + 4'-phosphopantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-4'-phosphopantetheine | - |
? | |
ATP + fatty acid + dephospho-CoA | - |
Rattus norvegicus | AMP + diphosphate + acyl-dephospho-CoA | - |
? | |
ATP + fatty acid + pantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-pantetheine | - |
? | |
ATP + long-chain carboxylic acid + CoA | substrate specificity of microsomal and mitochondrial enzyme are indistinguishable | Rattus norvegicus | AMP + diphosphate + long-chain acyl-CoA | - |
? | |
ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? | |
dATP + fatty acid + CoA | - |
Rattus norvegicus | dAMP + diphosphate + acyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
2.5 min: 17-20% loss of activity, 5 min: 33-37% loss of activity, 15 min: 58-59% loss of activity | Rattus norvegicus |