Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | isoform Facl1 shows 1.3% activity and isoform Facl2 shows 232% activity at 1% (v/v) | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | isoform Facl1 shows 64% residual activity and isoform Facl2 shows 71% residual activity at 1 mM | Geobacillus thermodenitrificans | |
Co2+ | isoform Facl1 shows 50% residual activity at 1 mM | Geobacillus thermodenitrificans | |
Cu2+ | isoform Facl1 shows 64% residual activity and isoform Facl2 shows 33% residual activity at 1 mM | Geobacillus thermodenitrificans | |
EDTA | isoform Facl1 shows 12% residual activity and isoform Facl2 shows 26% residual activity at 1 mM | Geobacillus thermodenitrificans | |
Fe2+ | isoform Facl1 shows 57% residual activity and isoform Facl2 shows 36% residual activity at 1 mM | Geobacillus thermodenitrificans | |
Mn2+ | isoform Facl1 shows 28% residual activity and isoform Facl2 shows 40% residual activity at 1 mM | Geobacillus thermodenitrificans | |
Ni2+ | isoform Facl1 shows 1.3% residual activity and isoform Facl2 shows no activity at 1 mM | Geobacillus thermodenitrificans | |
SDS | isoform Facl1 shows 1.3% residual activity and isoform Facl2 shows 2.6% residual activity at 0.05% (w/v) | Geobacillus thermodenitrificans | |
Zn2+ | isoform Facl1 shows 13% residual activity and isoform Facl2 shows 0.36% residual activity at 1 mM | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
caproate | isoform Facl1, at pH 7.5 and 60°C | Geobacillus thermodenitrificans | |
0.032 | - |
palmitate | isoform Facl2, at pH 7.5 and 60°C | Geobacillus thermodenitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Geobacillus thermodenitrificans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoform Facl1 shows 156% activity and isoform Facl2 shows 193% activity at 1 mM | Geobacillus thermodenitrificans | |
Co2+ | isoform Facl2 shows 191% activity at 1 mM | Geobacillus thermodenitrificans | |
additional information | K+, NH4+ and Na+ show no effects on the enzyme activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE | Geobacillus thermodenitrificans |
118000 | - |
gel filtration | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel chelating Sepharose column chromatography | Geobacillus thermodenitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
isoform Facl1, using caproate as substrate, at pH 7.5 and 60°C | Geobacillus thermodenitrificans |
8.7 | - |
isoform Facl2, using palmitate as substrate, at pH 7.5 and 60°C | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | isoform Facl1 exhibits less than 10% activity and isoform Facl2 exhibits about 20% activity | Geobacillus thermodenitrificans | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | isoform Facl1 exhibits less than 10% activity and isoform Facl2 exhibits about 20% activity | Geobacillus thermodenitrificans NG80-2 | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + arachidonate + CoA | isoform Facl1 exhibits about 40% activity and isoform Facl2 exhibits about 60% activity | Geobacillus thermodenitrificans | AMP + diphosphate + arachidonoyl-CoA | - |
? | |
ATP + arachidonate + CoA | isoform Facl1 exhibits about 40% activity and isoform Facl2 exhibits about 60% activity | Geobacillus thermodenitrificans NG80-2 | AMP + diphosphate + arachidonoyl-CoA | - |
? | |
ATP + behenoate + CoA | isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 65% activity | Geobacillus thermodenitrificans | AMP + diphosphate + behenoyl-CoA | - |
? | |
ATP + butyrate + CoA | isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 40% activity | Geobacillus thermodenitrificans | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + butyrate + CoA | isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 40% activity | Geobacillus thermodenitrificans NG80-2 | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + caproate + CoA | most preferred substrate for isoform Facl1 (100% activity) | Geobacillus thermodenitrificans | AMP + diphosphate + caproyl-CoA | - |
? | |
ATP + capryloate + CoA | isoform Facl1 exhibits about 50% activity and isoform Facl2 exhibits about 45% activity | Geobacillus thermodenitrificans | AMP + diphosphate + capryloyl-CoA | - |
? | |
ATP + hexacosanoate + CoA | isoform Facl1 exhibits about 8% activity and isoform Facl2 exhibits about 23% activity | Geobacillus thermodenitrificans | AMP + diphosphate + hexacosanoyl-CoA | - |
? | |
ATP + laurate + CoA | isoform Facl1 exhibits about 90% activity and isoform Facl2 exhibits about 55% activity | Geobacillus thermodenitrificans | AMP + diphosphate + lauryl-CoA | - |
? | |
ATP + lignocerate + CoA | isoform Facl1 exhibits about 20% activity and isoform Facl2 exhibits about 30% activity | Geobacillus thermodenitrificans | AMP + diphosphate + lignoceryl-CoA | - |
? | |
ATP + linoleate + CoA | - |
Geobacillus thermodenitrificans | AMP + diphosphate + linoleoyl-CoA | - |
? | |
ATP + melissoate + CoA | isoform Facl1 exhibits about 3% activity and isoform Facl2 exhibits about 1% activity | Geobacillus thermodenitrificans | AMP + diphosphate + melissoyl-CoA | - |
? | |
ATP + myristate + CoA | isoform Facl1 exhibits about 65% activity and isoform Facl2 exhibits about 85% activity | Geobacillus thermodenitrificans | AMP + diphosphate + myristoyl-CoA | - |
? | |
ATP + octacosanoate + CoA | isoform Facl1 exhibits about 5% activity and isoform Facl2 exhibits about 3% activity | Geobacillus thermodenitrificans | AMP + diphosphate + octacosanoyl-CoA | - |
? | |
ATP + palmitate + CoA | most preferred substrate for isoform Facl2 (100% activity) | Geobacillus thermodenitrificans | AMP + diphosphate + palmitoyl-CoA | - |
? | |
ATP + palmitate + CoA | most preferred substrate for isoform Facl2 (100% activity) | Geobacillus thermodenitrificans NG80-2 | AMP + diphosphate + palmitoyl-CoA | - |
? | |
ATP + stearate + CoA | isoform Facl1 exhibits about 45% activity and isoform Facl2 exhibits about 75% activity | Geobacillus thermodenitrificans | AMP + diphosphate + stearyl-CoA | - |
? | |
additional information | unsaturated fatty acids such as 14:1, 16:1, 18:1, 22:1, 18:3(n-3), 20:3(n-6) fatty acids and arachidonic acid are no substrates of isoform Facl1 or Facl2 | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | unsaturated fatty acids such as 14:1, 16:1, 18:1, 22:1, 18:3(n-3), 20:3(n-6) fatty acids and arachidonic acid are no substrates of isoform Facl1 or Facl2 | Geobacillus thermodenitrificans NG80-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59000, SDS-PAGE | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
Facl1 | isoform | Geobacillus thermodenitrificans |
Facl2 | isoform | Geobacillus thermodenitrificans |
fatty acid CoA synthetase, AMP forming | - |
Geobacillus thermodenitrificans |
long-chain fatty acid CoA ligase | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
about 40% of the highest activity of isoform Facl1 still remains after incubation at 60°C for 16 h, and above 50% of the highest isoform Facl2 activity still remains after incubation at 60°C for 8 h | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Geobacillus thermodenitrificans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | at pH levels from 5.0 to 11.0, isoform Facl activity increases along with an increase of pH, with the maximum activity obtained at pH 7.5, and about 40% of the highest activity still remains at pH 10.0 for isoform Facl1, and about 40% of the highest activity still remains at pH 9.5 for isoform Facl2 | Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus thermodenitrificans |
Organism | Comment | Expression |
---|---|---|
Geobacillus thermodenitrificans | there is a 6.7fold increase and a 10.8fold increase in the mRNA levels of isoforms facl1 and facl2, respectively, when crude oil is used as a sole carbon source instead of sucrose | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.6 | - |
palmitate | isoform Facl2, at pH 7.5 and 60°C | Geobacillus thermodenitrificans | |
37.5 | - |
caproate | isoform Facl1, at pH 7.5 and 60°C | Geobacillus thermodenitrificans |