Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E361A | inactive mutant enzyme | Escherichia coli |
G216A | the ratio of turnover number to Km-value for the reaction with oleate is 32.7% of the wild-type ratio | Escherichia coli |
G219A | the ratio of turnover number to Km-value for the reaction with oleate is 1.3fold higher than the wild-type ratio | Escherichia coli |
K222A | the ratio of turnover number to Km-value for the reaction with oleate is 8% of the wild-type ratio | Escherichia coli |
T214A | the ratio of turnover number to Km-value for the reaction with oleate is 10.3% of the wild-type ratio | Escherichia coli |
T217A | the ratio of turnover number to Km-value for the reaction with oleate is 6.7% of the wild-type ratio | Escherichia coli |
Y213A | inactive mutant enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ATP | pH 7.5, 37°C, mutant enzyme G219A | Escherichia coli | |
0.11 | - |
ATP | pH 7.5, 37°C, mutant enzyme G216A | Escherichia coli | |
0.13 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.13 | - |
ATP | pH 7.5, 37°C, mutant enzyme T214A | Escherichia coli | |
0.44 | - |
ATP | pH 7.5, 37°C, mutant enzyme K222A | Escherichia coli | |
0.53 | - |
ATP | pH 7.5, 37°C, mutant enzyme T217A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme plays a central role in intermediary metabolism by catalyzing the formation of acyl-CoA. The enzyme functions in the vectorial movement of exogenous fatty acids across the plasma membrane by acting as a metabolic trap, which results in the formation of acyl-CoA esters | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + decanoate + CoA | - |
Escherichia coli | AMP + diphosphate + decanoyl-CoA | - |
? | |
ATP + oleate + CoA | - |
Escherichia coli | AMP + diphosphate + oleoyl-CoA | - |
? | |
additional information | the enzyme plays a central role in intermediary metabolism by catalyzing the formation of acyl-CoA. The enzyme functions in the vectorial movement of exogenous fatty acids across the plasma membrane by acting as a metabolic trap, which results in the formation of acyl-CoA esters | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FACS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
ATP | pH 7.5, 37°C, mutant enzyme T214A | Escherichia coli | |
0.008 | - |
ATP | pH 7.5, 37°C, mutant enzyme G216A | Escherichia coli | |
0.008 | - |
ATP | pH 7.5, 37°C, mutant enzyme K222A | Escherichia coli | |
0.008 | - |
ATP | pH 7.5, 37°C, mutant enzyme T217A | Escherichia coli | |
0.017 | - |
ATP | pH 7.5, 37°C, mutant enzyme G219A | Escherichia coli | |
0.029 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | Escherichia coli |