Information on EC 3.6.1.9 - nucleotide diphosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.6.1.9
-
RECOMMENDED NAME
GeneOntology No.
nucleotide diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a nucleoside triphosphate + H2O = a nucleotide + diphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Nicotinate and nicotinamide metabolism
-
-
Pantothenate and CoA biosynthesis
-
-
Purine metabolism
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis I
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis II
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis III
-
-
pyrimidine deoxyribonucleotides dephosphorylation
-
-
Pyrimidine metabolism
-
-
Riboflavin metabolism
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-
Starch and sucrose metabolism
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-
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
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-
tunicamycin biosynthesis
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-
non-pathway related
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
dinucleotide nucleotidohydrolase
The enzyme preferentially hydrolyses ATP, but can also hydrolyse other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates. In vitro the enzyme also acts as a nucleotidohydrolase on ADP, NAD+, NADP+, FAD, and CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-64-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
-
Manually annotated by BRENDA team
var. botrytis
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
AGPPase1; AGPPase2
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Ovis aries aries
sheep
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
baker's yeast
-
-
Manually annotated by BRENDA team
hybrid of Saccharomyces fragilis x Saccharomyces dobzhanskii
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
cf. EC 3.1.4.1
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-dihydronicotinamide adenine dinucleotide + H2O
1,4-dihydronicotinamide mononucleotide + 5'-AMP
show the reaction diagram
2',3'-cAMP + H2O
adenosine monophosphate
show the reaction diagram
2'-5'-ApU + H2O
adenosine + uridine 5'-phosphate
show the reaction diagram
-
-
-
?
2'-5'-oligoadenylate + H2O
?
show the reaction diagram
-
-
-
-
?
2-nitrophenyl phosphate + H2O
2-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
3 diadenosine 5',5''-P1, P4-tetraphosphate + 3 H2O
2 ATP + 2 ADP + 2 AMP
show the reaction diagram
-
-
-
?
3',5'-cAMP + H2O
adenosine monophosphate
show the reaction diagram
-
traces of activity
-
?
3'-5'-ApA + H2O
adenosine + adenosine 5'-phosphate
show the reaction diagram
-
-
-
?
3'-5'-ApC + H2O
adenosine + cytidine 5'-phosphate
show the reaction diagram
-
-
-
?
3'-5'-ApU + H2O
adenosine + uridine 5'-phosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
3'-phosphoadenosine + phosphosulfate
show the reaction diagram
3-acetylpyridine adenine dinucleotide + H2O
3-acetylpyridine riboside 5'-phosphate + 5'-AMP
show the reaction diagram
3-aminopyridine 1,N6-ethenoadenine dinucleotide + H2O
3-aminopyridine riboside + N6-etheneadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
3-aminopyridine adenine dinucleotide + H2O
3-aminopyridine riboside 5'-phosphate + 5'-AMP
show the reaction diagram
3-methylpyridine adenine dinucleotide + H2O
3-methylpyridine mononucleotide + 5'-AMP
show the reaction diagram
-
-
-
?
3-pyridylcarbinol adenine dinucleotide + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
4-nitrophenyl phosphate dianion + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate monoanion + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
72fold higher activity than with 4-nitrophenyl phosphate dianion
-
-
?
4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
show the reaction diagram
4-nitrophenyl-TMP + H2O
4-nitrophenol + TMP
show the reaction diagram
-
-
-
-
?
5'-adenylylimidodiphosphate + H2O
?
show the reaction diagram
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
5'-deoxy-5'-thioadenosine 5'-triphosphate + H2O
5'-deoxy-5'-thioadenosine 3'-monophosphate + phosphate
show the reaction diagram
-
-
-
?
7-methyl 5'-guanosine 2'-O-methylgunaosine triphosphate + H2O
7-methyl-GMP + 7-methyl-GDP + GMP + GDP
show the reaction diagram
-
-
-
?
8-bromo-NAD+ + H2O
8-bromo-NMN + AMP
show the reaction diagram
-
same hydrolysis rate as for NAD+
-
?
adenosine (alpha,beta-methylene) 5'-triphosphate + H2O
adenosine (alpha,beta-methylene) 5'-diphosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-(alpha-naphthylphosphate)
adenosine + alpha-naphthylphosphate
show the reaction diagram
-
very low activity
-
?
adenosine 5'-O-(2-thiotriphosphate) + H2O
AMP + ?
show the reaction diagram
adenosine 5'-O-(3-thiotriphosphate) + H2O
AMP + ?
show the reaction diagram
-
-
-
?
adenosine 5'-phosphosulfate + H2O
?
show the reaction diagram
adenosine 5'-phosphosulfate + H2O
adenosine + phosphosulfate
show the reaction diagram
-
-
-
?
ADP + H2O
?
show the reaction diagram
ADP + H2O
AMP + phosphate
show the reaction diagram
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
show the reaction diagram
ADP-glucose + H2O
AMP + alpha-D-glucose-1-phosphate
show the reaction diagram
ADP-ribose + H2O
AMP + alpha-D-ribose 1-phosphate
show the reaction diagram
99% of the activity with ADP-glucose
-
-
?
ADP-ribose + H2O
AMP + phosphoribose
show the reaction diagram
alpha-NADH + H2O
alpha-NMNH + AMP
show the reaction diagram
-
low activity
-
?
alpha-naphthylphosphate + H2O
alpha-naphthol + phosphate
show the reaction diagram
-
low activity
-
?
ATP + 2 H2O
AMP + 2 phosphate
show the reaction diagram
-
-
-
?
ATP + H2O
?
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O
AMP + diphosphate
show the reaction diagram
ATP + H2O
diphosphate + AMP
show the reaction diagram
-
best substrate
-
-
?
beta,gamma-methyleneadenosine 5'-triphosphate + H2O
adenosine 5'-monophosphate + phosphomethylposphoric acid
show the reaction diagram
beta-naphthylphenylphosphonate + H2O
?
show the reaction diagram
-
very low activity
-
?
bis(4-nitrophenyl) phosphate + H2O
4-nitrophenylphosphate + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
bis(4-nitrophenyl)phosphate + H2O
?
show the reaction diagram
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenylphosphate
show the reaction diagram
bis-4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
2000fold higher activity than with 4-nitrophenyl phosphate dianion
-
-
?
bis-alpha-naphthylphosphate + H2O
?
show the reaction diagram
-
very low activity
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
CDP + H2O
?
show the reaction diagram
56% of the activity with UDP
-
-
?
CDP + H2O
CMP + phosphate
show the reaction diagram
CDP-choline + H2O
CMP + phosphorylcholine
show the reaction diagram
-
-
-
?
CDP-glucose + H2O
CMP + alpha-D-glucose 1-phosphate
show the reaction diagram
77% of the activity with ADP-glucose
-
-
?
CDP-glucose + H2O
CMP + D-glucose-1-phosphate
show the reaction diagram
coenzyme A + H2O
?
show the reaction diagram
-
low activity
-
?
CTP + H2O
?
show the reaction diagram
-
low activity
-
?
CTP + H2O
CDP + phosphate
show the reaction diagram
dATP + H2O
?
show the reaction diagram
-
-
-
?
dATP + H2O
dADP + phosphate
show the reaction diagram
-
-
-
-
?
dCDP + H2O
dCMP + phosphate
show the reaction diagram
-
-
-
-
?
dCTP + H2O
dCDP + phosphate
show the reaction diagram
-
-
-
-
?
dGDP + H2O
dGMP + phosphate
show the reaction diagram
-
-
-
-
?
dGTP + H2O
dGDP + phosphate
show the reaction diagram
-
-
-
-
?
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano + 2 H2O
?
show the reaction diagram
low hydrolysis activity with the inhibitor as substrate
-
-
?
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate + 2 H2O
?
show the reaction diagram
low hydrolysis activity with the inhibitor as substrate
-
-
?
diadenosine 5',5''-P1, P2-diphosphate + H2O
AMP + AMP
show the reaction diagram
diadenosine 5',5''-P1, P3-triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
?
diadenosine 5',5''-P1, P5-pentaposphate + H2O
ATP + ADP + AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
?
diadenosine 5',5''-P1,P3-triphosphate + H2O
5'-ADP + 5'-AMP
show the reaction diagram
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
ATP + 5'-AMP
show the reaction diagram
diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano + 2 H2O
?
show the reaction diagram
low hydrolysis activity with the inhibitor as substrate
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
p4A + 5'-AMP
show the reaction diagram
diadenosine 5'-diphosphate + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
diadenosine polyphosphate + H2O
AMP + ?
show the reaction diagram
-
from di- to pentaphosphates, hydrolysis rate decreases with chain length
-
?
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
GTP + 5'-GMP
show the reaction diagram
diphosphate + H2O
2 phosphate
show the reaction diagram
-
significant activity
-
?
diphosphate + H2O
?
show the reaction diagram
diphosphate + H2O
phosphate
show the reaction diagram
-
-
-
-
?
diphospho-3-acetylpyridine nucleotide + H2O
?
show the reaction diagram
-
reduced form, 2fold higher activity than for NAD+
-
?
diphospho-deaminopyridine nucleotide + H2O
?
show the reaction diagram
-
-
-
?
DNA + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
E. coli, low activity
-
?
dTTP + H2O
dTMP + diphosphate
show the reaction diagram
the enzyme possesses dTTP-specific activity at a substrate concentration of 0.1 mM
-
-
?
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
weakest substrate
-
-
?
FAD + H2O
FMN + AMP
show the reaction diagram
formycin 5'-triphosphate + H2O
?
show the reaction diagram
-
-
-
?
GDP + H2O
?
show the reaction diagram
38% of the activity with UDP
-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
GDP-glucose + H2O
GMP + D-glucose-1-phosphate
show the reaction diagram
GDP-mannose + H2O
GMP + alpha-D-mannose 1-phosphate
show the reaction diagram
76% of the activity with ADP-glucose
-
-
?
GDP-mannose + H2O
GMP + D-mannose-1-phosphate
show the reaction diagram
GTP + H2O
GDP + phosphate
show the reaction diagram
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
1.5fold more hydrolysis than for ATP
-
?
guanosine (alpha,beta-methylene) 5'-triphosphate + H2O
guanosine (alpha,beta-methylene) 5'-diphosphate + phosphate
show the reaction diagram
-
-
-
?
IDP + H2O
?
show the reaction diagram
48% of the activity with UDP
-
-
?
IDP + H2O
IMP + phosphate
show the reaction diagram
ITP + H2O
IDP + phosphate
show the reaction diagram
-
-
-
-
?
methyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + methyl phosphate
show the reaction diagram
-
200fold higher activity than with 4-nitrophenyl phosphate dianion
-
-
?
methyl 4-nitrophenyl phosphate diester + H2O
?
show the reaction diagram
-
-
-
-
?
mRNA + H2O
7-methyl-GMP + ?
show the reaction diagram
NAD+ + H2O
NMN + AMP
show the reaction diagram
NADH + H2O
NMNH + AMP
show the reaction diagram
NADP+ + H2O
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
show the reaction diagram
NADP+ + H2O
NMN + adenosine 2',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
NADP+ + H2O
NMN + adenosine 2',5'-phosphate
show the reaction diagram
NADPH + H2O
NMNH + adenosine 2',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
NADPH + H2O
NMNH + adenosine 2',5'-phosphate
show the reaction diagram
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
NMN + N6-ethenoadenosine 5'-phosphate
show the reaction diagram
nicotinamide guanine dinucleotide + H2O
NMN + GMP
show the reaction diagram
nicotinamide hypoxanthine dinucleotide + H2O
NMN + hypoxanthine monophosphate
show the reaction diagram
nicotinic acid adenine dinucleotide + H2O
nicotinic acid mononucleotide + 5'-AMP
show the reaction diagram
p-nitrophenyl 5'-thymidine monophosphate + H2O
4-nitrophenol + TMP
show the reaction diagram
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + phenylphosphonate
show the reaction diagram
p-nitrophenyl thymidine 5'-monophosphate + H2O
?
show the reaction diagram
-
-
-
?
p-nitrophenyl-dTMP + H2O
p-nitrophenol + dTMP
show the reaction diagram
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
P1,P2-di(adenosine-5') diphosphate + H2O
?
show the reaction diagram
-
tetramer 3fold more active than dimer
-
?
P1,P2-di(thymidine-3') diphosphate + H2O
?
show the reaction diagram
-
very low activity for both dimer and tetramer
-
?
P1,P2-di(thymidine-5') diphosphate + H2O
?
show the reaction diagram
-
low activity for both dimer and tetramer
-
?
P1,P3-di(adenosine-5') triphosphate + H2O
?
show the reaction diagram
-
tetramer 2fold more active than dimer
-
?
P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate + 2 H2O
?
show the reaction diagram
low hydrolysis activity with the inhibitor as substrate
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + 2 H2O
?
show the reaction diagram
-
-
-
?
P1-5'-(7-methyl)-guanosine-P3-5'-guanosine triphosphate + H2O
7-methylguanosine 5'-diphosphate + GMP
show the reaction diagram
-
cap analogue
-
?
poly (ADP-ribose) + H2O
phosphoribosyladenosine 5'-phosphate + ?
show the reaction diagram
-
-
-
?
polyadenylate + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
low activity
-
?
polynucleotide + H2O
nucleoside 5'-phosphate + ?
show the reaction diagram
-
hardly degraded
-
?
pyridine adenine dinucleotide + H2O
pyridine riboside 5'-phosphate + 5'-AMP
show the reaction diagram
RNA + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
yeast, low activity
-
?
TDP + H2O
?
show the reaction diagram
TDP-glucose + H2O
tMP + alpha-D-glucose 1-phosphate
show the reaction diagram
TDP-glucose + H2O
TMP + D-glucose-1-phosphate
show the reaction diagram
thiamine diphosphate + H2O
?
show the reaction diagram
-
-
-
?
thionicotinamide adenine dinucleotide + H2O
thionicotinamide mononucleotide + 5'-AMP
show the reaction diagram
-
-
-
?
thymidine + alpha-naphthylphosphate + H2O
thymidine 3'-alpha-naphthol + phosphate
show the reaction diagram
-
very low activity
-
?
thymidine 3'-diphospho 3'-thymidine + H2O
thymidine 3'-phosphate + thymidine 3'-phosphate
show the reaction diagram
thymidine 3'-monophosphate p-nitrophenyl ester + H2O
thymidine 3'-monophosphate + p-nitrophenol
show the reaction diagram
thymidine 5'-6-bromo-2-hydroxy-3-naphthoic acid 2-methoxyanilide-naphthylphosphate + H2O
?
show the reaction diagram
-
low activity
-
?
thymidine 5'-alpha-naphthylphosphate + H2O
thymidine + alpha-naphthylphosphate
show the reaction diagram
thymidine 5'-diphospho 5'-thymidine + H2O
thymidine 5'-phosphate + thymidine 5'-phosphate
show the reaction diagram
-
low activity
-
?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
thymidine 5'-monophosphate + p-nitrophenol
show the reaction diagram
thymidine-5'-L-monophosphate p-nitrophenyl ester + H2O
thymidine 5'-monophosphate + p-nitrophenol
show the reaction diagram
-
-
-
-
?
TTP + H2O
TDP + phosphate
show the reaction diagram
TTP + H2O
TMP + diphosphate
show the reaction diagram
the enzyme exhibits significant activity for TTP only when substrate concentration reaches 0.5 mM
-
-
?
UDP + H2O
?
show the reaction diagram
UDP + H2O
UMP + phosphate
show the reaction diagram
UDP-alpha-D-galactose + H2O
UMP + alpha-D-galactose-1-phosphate
show the reaction diagram
UDP-glucose + H2O
UMP + alpha-D-glucose 1-phosphate
show the reaction diagram
UDP-glucose + H2O
UMP + D-glucose-1-phosphate
show the reaction diagram
UDP-glucuronic acid + H2O
UMP + glucuronic acid phosphate
show the reaction diagram
UDP-MurNAc + H2O
UMP + ?
show the reaction diagram
UDP-MurNAc-peptide + H2O
UMP + ?
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + H2O
N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + H2O
UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
show the reaction diagram
UMP + H2O
uridine + phosphate
show the reaction diagram
-
very low activity
-
?
uridine 3'-(alpha-naphthylphosphate) + H2O
uridine + alpha-naphthylphosphate
show the reaction diagram
-
very low activity
-
?
uridine 3'-(phenylphosphate) + H2O
uridine + phenylphosphate
show the reaction diagram
-
very low activity
-
?
uridine diphosphoglucose + H2O
UMP + D-glucose-1-phosphate
show the reaction diagram
-
-
-
?
UTP + H2O
diphosphate + UMP
show the reaction diagram
-
-
-
-
?
UTP + H2O
UDP + phosphate
show the reaction diagram
-
highest rate of hydrolysis
-
-
?
UTP + H2O
UMO + diphosphate
show the reaction diagram
the enzyme exhibits significant activity for UTP only when substrate concentration reaches 0.5 mM
-
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
additional information
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2',3'-cAMP + H2O
adenosine monophosphate
show the reaction diagram
2'-5'-oligoadenylate + H2O
?
show the reaction diagram
-
-
-
-
?
2-nitrophenyl phosphate + H2O
2-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
3 diadenosine 5',5''-P1, P4-tetraphosphate + 3 H2O
2 ATP + 2 ADP + 2 AMP
show the reaction diagram
-
-
-
?
3',5'-cAMP + H2O
adenosine monophosphate
show the reaction diagram
-
traces of activity
-
?
4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
show the reaction diagram
O14638, P22413
hydrolysis of pnp-TMP at the cell surface of both osteocarcinoma and colon cancer cells
-
-
?
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
traces of activity
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
show the reaction diagram
ADP-ribose + H2O
AMP + phosphoribose
show the reaction diagram
alpha-NADH + H2O
alpha-NMNH + AMP
show the reaction diagram
-
low activity
-
?
ATP + H2O
AMP + diphosphate
show the reaction diagram
CDP + H2O
CMP + phosphate
show the reaction diagram
-
low activity
-
?
CDP-choline + H2O
CMP + phosphorylcholine
show the reaction diagram
-
-
-
?
CDP-glucose + H2O
CMP + D-glucose-1-phosphate
show the reaction diagram
coenzyme A + H2O
?
show the reaction diagram
-
low activity
-
?
CTP + H2O
?
show the reaction diagram
-
low activity
-
?
dATP + H2O
?
show the reaction diagram
-
-
-
?
diadenosine 5',5''-P1, P2-diphosphate + H2O
AMP + AMP
show the reaction diagram
diadenosine 5',5''-P1, P3-triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
?
diadenosine 5',5''-P1, P5-pentaposphate + H2O
ATP + ADP + AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
?
DNA + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
E. coli, low activity
-
?
FAD + H2O
FMN + AMP
show the reaction diagram
GDP + H2O
GMP + phosphate
show the reaction diagram
-
low activity
-
?
GDP-glucose + H2O
GMP + D-glucose-1-phosphate
show the reaction diagram
-
-
-
?
GDP-mannose + H2O
GMP + D-mannose-1-phosphate
show the reaction diagram
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
1.5fold more hydrolysis than for ATP
-
?
IDP + H2O
IMP + phosphate
show the reaction diagram
-
-
-
?
mRNA + H2O
7-methyl-GMP + ?
show the reaction diagram
NAD+ + H2O
NMN + AMP
show the reaction diagram
NADH + H2O
NMNH + AMP
show the reaction diagram
NADP+ + H2O
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
show the reaction diagram
NADP+ + H2O
NMN + adenosine 2',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
NADP+ + H2O
NMN + adenosine 2',5'-phosphate
show the reaction diagram
NADPH + H2O
NMNH + adenosine 2',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
NADPH + H2O
NMNH + adenosine 2',5'-phosphate
show the reaction diagram
poly (ADP-ribose) + H2O
phosphoribosyladenosine 5'-phosphate + ?
show the reaction diagram
-
-
-
?
polyadenylate + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
low activity
-
?
polynucleotide + H2O
nucleoside 5'-phosphate + ?
show the reaction diagram
-
hardly degraded
-
?
RNA + H2O
adenosine 5'-monophosphate + ?
show the reaction diagram
-
yeast, low activity
-
?
TDP-glucose + H2O
TMP + D-glucose-1-phosphate
show the reaction diagram
thiamine diphosphate + H2O
?
show the reaction diagram
-
-
-
?
UDP + H2O
UMP + phosphate
show the reaction diagram
UDP-alpha-D-galactose + H2O
UMP + alpha-D-galactose-1-phosphate
show the reaction diagram
UDP-glucose + H2O
UMP + D-glucose-1-phosphate
show the reaction diagram
UDP-glucuronic acid + H2O
UMP + glucuronic acid phosphate
show the reaction diagram
UDP-MurNAc + H2O
UMP + ?
show the reaction diagram
UDP-MurNAc-peptide + H2O
UMP + ?
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + H2O
N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + H2O
UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
show the reaction diagram
UMP + H2O
uridine + phosphate
show the reaction diagram
-
very low activity
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
2-5 mM restores activity of EDTA-inhibited enzyme
MgCl2
2-5 mM partially restores activity of EDTA-inhibited enzyme
ZnCl2
2-5 mM restores activity of EDTA-inhibited enzyme
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',3'-cAMP
-
7% inhibition at 2 mM
2',3'-dialdehyde ATP
-
competitive inhibition
2,3-Butanedione
-
inactivation half-life: 21.8 min at 0.1 mM
2,3-dimercapto-1-propanol
2-mercaptoethanol
2-methylthioadenosine 5'-triphosphate
-
competitive inhibition
3',5'-cAMP
-
4% inhibition at 2 mM
3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate
-
competitive inhibition
4,4'-diisothiocyanatostilbene-2,2' disulfonic acid
-
P2-purinoceptor antagonist, less potent inhibition, IC50: 0.022 mM
5'-ADP
5'-AMP
5'-phosphoadenosine 3'-phosphate
-
P2-purinoceptor antagonist, less potent inhibitor, IC50: 0.036 mM
acidic fibroblast growth factor
-
potent inhibition at nearly stoichiometric concentrations, Ki: 1.3 nM
-
adenosine
-
competitive inhibition, Ki: 2.7 mM
adenosine 2'-monophosphate
-
8% inhibition at 2 mM
adenosine 3',5'-bisphosphate
-
-
adenosine 3'-monophosphate
adenosine 5'-sulfate
Adenyl-5'-yl imidodiphosphate
adenylic acid
-
competitive inhibition, Ki: 0.036 mM
ADP-ribose
-
47% inhibition at 0.5 mM, competitive type of inhibition
alpha,beta-methylene adenosine 5'-triphosphate
alpha,beta-methylene ADP
-
mixed inhibition
alpha,beta-methylene ATP
-
competitive inhibition
AMP(CH)2PP
-
competitive inhibition, Ki: 25 mM
AMP-amidate
-
competitive inhibition, Ki: 30 mM
AMP-PP(S)
-
competitive inhibition, Ki: 33 mM
B4 lectin
-
from Vicia villosa, strong inhibition between 0.002-0.02 mM, specific for hydrolysis of UDP-N-acetyl-alpha-D-galactosamine, reversal by addition of N-acetyl-alpha-D-galactosamine, only weakly inhibitory towards UDP-galactose or UDP-GlcNAc
-
beta,gamma-methyleneadenosine 5'-triphosphate
-
competitive inhibition of formycin 5'-triphosphate hydrolysis, Ki: 0.1 mM
Borate
-
strong inhibition at pH 8.5, not at pH 7.4
CDP-choline
-
62% inhibition at 0.2 mM
Cibacron blue
coenzyme A
-
90% inhibition at 0.2 mM
CTP
Ovis aries aries
-
potent inhibition at 0.1 mM
cytidine 5'-diphosphate
-
competitive inhibition, Ki: 0.0085 mM
dAMP
-
16% inhibition at 17 mM
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano
-
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate
-
diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano
-
diethyldiphosphate
-
inactivation follows apparent first-order kinetics, completely restored by hydroxylamine
diphosphate
dithiothreitol
EGTA
-
strong inhibition at 1 mM, partially reversed by Mg2+, complete reactivation at 10 mM Ca2+
F-
-
0.01 mM, 54% inhibition
FeSO4
Ovis aries aries
-
42% inhibition at 1 mM in the presence of 0.1 mM o-phenanthroline
GDP
-
competitive inhibition, Ki: 0.0025 mM
GDP-mannose
-
competitive inhibition of phosphodiesterase activity
glycine
-
-
GSH
Ovis aries aries
-
40% inhibition at 1 mM
GSSG
Ovis aries aries
-
25% inhibition at 1 mM
guanidine hydrochloride
-
85% inhibition at 4.2 M, complete recovery of activity after dialysis
heparan sulfate
-
NPP1
heparin
-
NPP1
ITP
Ovis aries aries
-
potent inhibition at 0.1 mM
L-ascorbic acid
-
8% inhibition at 16 mM
L-cysteine
L-phenylalanine
-
5 mM, 10% loss of activity
lectin
-
from Wistaria floribunda, inhibition for hydrolysis of UDP-N-acetyl-alpha-D-galactosamine, reversal by addition of N-acetyl-alpha-D-galactosamine
-
N-ethyl-5-phenylisoxazolium-3'-sulfonate
-
Woodward's reagent, inactivation half-life: 16 min at 0.05 mM
N-omega-nitro-L-arginine methyl ester
-
enzyme activity is decreased in serum and platelets from N-omega-nitro-L-arginine methyl ester -treated rats
N1-butylnicotinamide chloride
-
competitive inhibition, Ki: 0.408 mM
N1-heptylnicotinamide chloride
-
competitive inhibition, Ki: 0.48 mM
N1-hexylnicotinamide chloride
-
competitive inhibition, Ki: 0.629 mM
N1-octylnicotinamide chloride
-
competitive inhibition, Ki: 0.287 mM
N1-pentylnicotinamide chloride
-
competitive inhibition, Ki: 0.297 mM
nicotinamide
-
19% inhibition at 100 mM
NMN
-
10% inhibition at 40 mM
nucleoside monophosphates
-
-
o-phenanthroline
p-hydroxymercuribenzoate
Ovis aries aries
-
60% inhibition at 1 mM, reversal by GSH
p-nitrophenyl 3'-thymidylate
-
15% inhibition at 0.2 mM
p-nitrophenyl 5'-thymidylate
-
15% inhibition at 0.2 mM
P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate
-
phosphate
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
pyridoxal phosphate-6-azophenyl-2',4'disulfonic acid
Reactive blue 2
SDS
-
partial inactivation at 0.1%, w/v, complete inactivation at 1%, w/v
Sodium diphosphate
Sodium fluoride
-
about 30% residual activity at 40 mM sodium fluoride using ATP as substrate, less than 60% residual activity at 40 mM sodium fluoride using ADP or bis(4-nitrophenyl) phosphate as substrate
suramin
thiamine diphosphate
-
17% inhibition at 0.2 mM
TTP
Ovis aries aries
-
70% inhibition at 1 mM
UDP-galactose
-
competitive inhibition, Ki: 29 mM
UDP-glucose
uridine 5'-diphosphate
-
competitive inhibition, Ki: 0.0012 mM
UTP
Ovis aries aries
-
potent inhibition at 0.1 mM
additional information
-