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Literature summary for 3.6.1.9 extracted from

  • Spano, D.; Pintus, F.; Pes, R.; Medda, R.; Floris, G.
    Purification and characterisation of a soluble nucleotide pyrophosphatase/phosphodiesterase from prickly pear (Opuntia ficus indica) fruits (2011), Food Res. Int., 44, 2264-2270.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA EDTA is not able to remove the bound metal ions in ONPP Opuntia ficus-indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
4-nitrophenyl-5'-TMP pH 8.0, 25°C Opuntia ficus-indica
0.38
-
NADP+ pH 8.0, 25°C Opuntia ficus-indica
0.65
-
NADPH pH 8.0, 25°C Opuntia ficus-indica
3.2
-
NAD+ pH 8.0, 25°C Opuntia ficus-indica
4.4
-
NADH pH 8.0, 25°C Opuntia ficus-indica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Opuntia ficus-indica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ metalloprotein, subunits each containing one Ca2+ and one Mg2+ ion Opuntia ficus-indica
Mg2+ metalloprotein, subunits each containing one Ca2+ and one Mg2+ ion Opuntia ficus-indica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, about, SDS-PAGE Opuntia ficus-indica
105000
-
gel filtration Opuntia ficus-indica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Opuntia ficus-indica
-
NMN + AMP
-
?
NADH + H2O Opuntia ficus-indica
-
NMNH + AMP
-
?
NADP+ + H2O Opuntia ficus-indica
-
NMN + adenosine 2',5'-bisphosphate
-
?
NADPH + H2O Opuntia ficus-indica
-
NMNH + adenosine 2',5'-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Opuntia ficus-indica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains about 9-10% carbohydrates Opuntia ficus-indica

Purification (Commentary)

Purification (Comment) Organism
soluble native enzyme 618fold from fruits to homogeneity by ammonium sulfate fractionation, heat treatment at 75°C, anion exchange chromatography, and gel filtration Opuntia ficus-indica

Source Tissue

Source Tissue Comment Organism Textmining
fruit increase of enzyme activity during ripening from the green to the red stage of fruits development Opuntia ficus-indica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
420
-
purified native enzyme, pH 8.0, 25°C Opuntia ficus-indica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-5'-TMP + H2O
-
Opuntia ficus-indica 4-nitrophenol + 5'-TMP
-
?
additional information substrate specificity, overview. No activity with bis-4-nitrophenyl phosphate. FAD, ADP-ribose, ADPG, UDPG, some diadenosine polyphosphates (ApnAs), ATP, ADP, UDP and 3',5'-cAMP are poor or no substrates Opuntia ficus-indica ?
-
?
NAD+ + H2O
-
Opuntia ficus-indica NMN + AMP
-
?
NADH + H2O
-
Opuntia ficus-indica NMNH + AMP
-
?
NADP+ + H2O
-
Opuntia ficus-indica NMN + adenosine 2',5'-bisphosphate
-
?
NADPH + H2O
-
Opuntia ficus-indica NMNH + adenosine 2',5'-bisphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, about, SDS-PAGE Opuntia ficus-indica

Synonyms

Synonyms Comment Organism
nucleotide pyrophosphatase/phosphodiesterase
-
Opuntia ficus-indica
ONPP
-
Opuntia ficus-indica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Opuntia ficus-indica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
purified native enzyme, 30 min, stable Opuntia ficus-indica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
110
-
NADP+ pH 8.0, 25°C Opuntia ficus-indica
197
-
NAD+ pH 8.0, 25°C Opuntia ficus-indica
745
-
NADPH pH 8.0, 25°C Opuntia ficus-indica
828
-
NADH pH 8.0, 25°C Opuntia ficus-indica
1675
-
4-nitrophenyl-5'-TMP pH 8.0, 25°C Opuntia ficus-indica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Opuntia ficus-indica

pH Range

pH Minimum pH Maximum Comment Organism
7 11 activity range, analysis of pH-dependence within this range, overview. No activity above pH 10.5 Opuntia ficus-indica

General Information

General Information Comment Organism
physiological function Opuntia NPP might be involved in fruit ripening Opuntia ficus-indica