Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | EDTA is not able to remove the bound metal ions in ONPP | Opuntia ficus-indica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
4-nitrophenyl-5'-TMP | pH 8.0, 25°C | Opuntia ficus-indica | |
0.38 | - |
NADP+ | pH 8.0, 25°C | Opuntia ficus-indica | |
0.65 | - |
NADPH | pH 8.0, 25°C | Opuntia ficus-indica | |
3.2 | - |
NAD+ | pH 8.0, 25°C | Opuntia ficus-indica | |
4.4 | - |
NADH | pH 8.0, 25°C | Opuntia ficus-indica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Opuntia ficus-indica | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | metalloprotein, subunits each containing one Ca2+ and one Mg2+ ion | Opuntia ficus-indica | |
Mg2+ | metalloprotein, subunits each containing one Ca2+ and one Mg2+ ion | Opuntia ficus-indica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, about, SDS-PAGE | Opuntia ficus-indica |
105000 | - |
gel filtration | Opuntia ficus-indica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + H2O | Opuntia ficus-indica | - |
NMN + AMP | - |
? | |
NADH + H2O | Opuntia ficus-indica | - |
NMNH + AMP | - |
? | |
NADP+ + H2O | Opuntia ficus-indica | - |
NMN + adenosine 2',5'-bisphosphate | - |
? | |
NADPH + H2O | Opuntia ficus-indica | - |
NMNH + adenosine 2',5'-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Opuntia ficus-indica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains about 9-10% carbohydrates | Opuntia ficus-indica |
Purification (Comment) | Organism |
---|---|
soluble native enzyme 618fold from fruits to homogeneity by ammonium sulfate fractionation, heat treatment at 75°C, anion exchange chromatography, and gel filtration | Opuntia ficus-indica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | increase of enzyme activity during ripening from the green to the red stage of fruits development | Opuntia ficus-indica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
420 | - |
purified native enzyme, pH 8.0, 25°C | Opuntia ficus-indica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-5'-TMP + H2O | - |
Opuntia ficus-indica | 4-nitrophenol + 5'-TMP | - |
? | |
additional information | substrate specificity, overview. No activity with bis-4-nitrophenyl phosphate. FAD, ADP-ribose, ADPG, UDPG, some diadenosine polyphosphates (ApnAs), ATP, ADP, UDP and 3',5'-cAMP are poor or no substrates | Opuntia ficus-indica | ? | - |
? | |
NAD+ + H2O | - |
Opuntia ficus-indica | NMN + AMP | - |
? | |
NADH + H2O | - |
Opuntia ficus-indica | NMNH + AMP | - |
? | |
NADP+ + H2O | - |
Opuntia ficus-indica | NMN + adenosine 2',5'-bisphosphate | - |
? | |
NADPH + H2O | - |
Opuntia ficus-indica | NMNH + adenosine 2',5'-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, about, SDS-PAGE | Opuntia ficus-indica |
Synonyms | Comment | Organism |
---|---|---|
nucleotide pyrophosphatase/phosphodiesterase | - |
Opuntia ficus-indica |
ONPP | - |
Opuntia ficus-indica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Opuntia ficus-indica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
purified native enzyme, 30 min, stable | Opuntia ficus-indica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
NADP+ | pH 8.0, 25°C | Opuntia ficus-indica | |
197 | - |
NAD+ | pH 8.0, 25°C | Opuntia ficus-indica | |
745 | - |
NADPH | pH 8.0, 25°C | Opuntia ficus-indica | |
828 | - |
NADH | pH 8.0, 25°C | Opuntia ficus-indica | |
1675 | - |
4-nitrophenyl-5'-TMP | pH 8.0, 25°C | Opuntia ficus-indica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Opuntia ficus-indica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | activity range, analysis of pH-dependence within this range, overview. No activity above pH 10.5 | Opuntia ficus-indica |
General Information | Comment | Organism |
---|---|---|
physiological function | Opuntia NPP might be involved in fruit ripening | Opuntia ficus-indica |