Cloned (Comment) | Organism |
---|---|
mutants expressed in COS-1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D200N | mutation abolishes formation of the nucleotidylated intermediate | Mus musculus |
D358N | mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis | Mus musculus |
F239A | mutation halves activity, decreased ability to trap the catalytic intermediate | Mus musculus |
F239Q | decreased activity and level of covalent intermediate of the mutant may be accounted for by a decreased binding of the substrate | Mus musculus |
H362Q | mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis | Mus musculus |
H406Q | mutation abolishes formation of the nucleotidylated intermediate | Mus musculus |
K237A | mutation halves activity | Mus musculus |
T238S | mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | activity can be restored by addition of 2-5 mM ZnCl2 or CaCl2, but only partially by 2-5 mM MgCl2 | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2-5 mM restores activity of EDTA-inhibited enzyme | Mus musculus | |
MgCl2 | 2-5 mM partially restores activity of EDTA-inhibited enzyme | Mus musculus | |
ZnCl2 | 2-5 mM restores activity of EDTA-inhibited enzyme | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9EQG7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | mutations D358N and H362Q still allow a weak labeling, whereas mutation His517, Asp200, Asp405 and His406 completely abolish autophosphorylation in presence of ATP | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
NPP1 | - |
Mus musculus |
nucleotide pyrophosphatase | - |
Mus musculus |