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Literature summary for 3.6.1.9 extracted from

  • Krishnan, N.; Appaji Rao, N.
    Studies on nucleotide pyrophosphatase I. Partial purification and properties of a sheep liver enzyme that catalyses the hydrolysis of dinucleotides (1972), Arch. Biochem. Biophys., 149, 336-348.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate enhanced release of FMN at 0.01 mM, increases Vmax Ovis aries aries

General Stability

General Stability Organism
complete inactivation if repeated freezed and thawed Ovis aries aries

Inhibitors

Inhibitors Comment Organism Structure
5'-ADP complete inhibition at 1 mM Ovis aries aries
5'-AMP sigmoid type of inhibition, phosphate abolishes sigmoidal response Ovis aries aries
ATP potent inhibition at 0.1 mM Ovis aries aries
CTP potent inhibition at 0.1 mM Ovis aries aries
diphosphate complete inhibition above 0.1 mM Ovis aries aries
EDTA
-
Ovis aries aries
FAD+ 50% inhibition at 1-2 mM Ovis aries aries
FeSO4 42% inhibition at 1 mM in the presence of 0.1 mM o-phenanthroline Ovis aries aries
GMP complete inhibition at 1 mM Ovis aries aries
GSH 40% inhibition at 1 mM Ovis aries aries
GSSG 25% inhibition at 1 mM Ovis aries aries
ITP potent inhibition at 0.1 mM Ovis aries aries
o-phenanthroline 60-90% inhibition at 0.1 mM, reversal by FeSO4 Ovis aries aries
p-hydroxymercuribenzoate 60% inhibition at 1 mM, reversal by GSH Ovis aries aries
TTP 70% inhibition at 1 mM Ovis aries aries
UTP potent inhibition at 0.1 mM Ovis aries aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
FAD with and without phosphate which abolishes negative cooperative interaction Ovis aries aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FAD + H2O Ovis aries aries
-
FMN + AMP
-
?
NAD+ + H2O Ovis aries aries
-
NMN + AMP
-
?
NADP+ + H2O Ovis aries aries
-
NMN + adenosine 2',5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Ovis aries aries
-
sheep
-

Purification (Commentary)

Purification (Comment) Organism
partial Ovis aries aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Ovis aries aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
107
-
-
Ovis aries aries

Storage Stability

Storage Stability Organism
5°C, 80% of activity remains after 16 h Ovis aries aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + H2O
-
Ovis aries aries FMN + AMP
-
?
NAD+ + H2O
-
Ovis aries aries NMN + AMP
-
?
NADP+ + H2O
-
Ovis aries aries NMN + adenosine 2',5'-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 36 first optimum Ovis aries aries
42
-
in the presence of phosphate Ovis aries aries
60
-
second optimum Ovis aries aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
-
Ovis aries aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1 2 FAD+
-
Ovis aries aries