EC Number |
Protein Variants |
Reference |
---|
3.6.1.9 | D200N |
mutation abolishes formation of the nucleotidylated intermediate |
659151 |
3.6.1.9 | D358N |
mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis |
659151 |
3.6.1.9 | DELTA804-905 |
mutation results in complete loss of activity, protein remains entirely intracellular |
657820 |
3.6.1.9 | E199A |
site-directed mutagenesis, the mutant shows increased activity with substrate 4-nitrophenyl phosphate compared to the wild-type |
718664 |
3.6.1.9 | E199D |
site-directed mutagenesis, the mutation doubles the enzyme activity against NAD(H) |
718664 |
3.6.1.9 | E322A |
site-directed mutagenesis |
719660 |
3.6.1.9 | E322Y |
site-directed mutagenesis |
719660 |
3.6.1.9 | E33A |
inactive |
735007 |
3.6.1.9 | E41Q/E42Q |
significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity |
748152 |
3.6.1.9 | E45Q |
significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity |
748152 |