Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | less than 20% residual activity at 40 mM dithiothreitol using ATP as substrate, less than 10% residual activity at 40 mM dithiothreitol using ADP as substrate, less than 50% residual activity at 40 mM dithiothreitol using bis(4-nitrophenyl) phosphate as substrate | Bothrops jararaca | |
EDTA | less than 20% residual activity at 40 mM EDTA using ATP as substrate, less than 10% residual activity at 40 mM EDTA using ADP as substrate, about 40% residual activity at 40 mM EDTA using bis(4-nitrophenyl) phosphate as substrate | Bothrops jararaca | |
additional information | phenylmethylsulfonyl fluoride does not modify the catalytic activity of NPP | Bothrops jararaca | |
Sodium fluoride | about 30% residual activity at 40 mM sodium fluoride using ATP as substrate, less than 60% residual activity at 40 mM sodium fluoride using ADP or bis(4-nitrophenyl) phosphate as substrate | Bothrops jararaca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
p-nitrophenyl 5'-thymidine monophosphate | in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca | |
21.88 | - |
bis(4-nitrophenyl) phosphate | in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
288000 | - |
gel filtration | Bothrops jararaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops jararaca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
blue-Sepharose column chromatography, oligo-dT cellulose column chromatography, and Sephacryl S-300 gel filtration | Bothrops jararaca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops jararaca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
crude enzyme, using ADP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
0.17 | - |
crude enzyme, using ATP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
0.67 | - |
crude enzyme, using bis(4-nitrophenyl) phosphate as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
1.3 | - |
after 8.3fold purification, using ADP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
10.3 | - |
after 61.6fold purification, using ATP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
242 | - |
after 362.5fold purification, using bis(4-nitrophenyl) phosphate as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 5fold lower activity compared to ATP | Bothrops jararaca | AMP + phosphate | - |
? | |
ATP + H2O | the specific activity of NPP for ATP is about 5fold higher than that of ADP | Bothrops jararaca | ADP + phosphate | - |
? | |
ATP + H2O | - |
Bothrops jararaca | AMP + diphosphate | - |
? | |
bis(4-nitrophenyl) phosphate + H2O | - |
Bothrops jararaca | 4-nitrophenylphosphate + 4-nitrophenol | - |
? | |
CDP + H2O | - |
Bothrops jararaca | CMP + phosphate | - |
? | |
CTP + H2O | - |
Bothrops jararaca | CDP + phosphate | - |
? | |
dATP + H2O | - |
Bothrops jararaca | dADP + phosphate | - |
? | |
dCDP + H2O | - |
Bothrops jararaca | dCMP + phosphate | - |
? | |
dCTP + H2O | - |
Bothrops jararaca | dCDP + phosphate | - |
? | |
dGDP + H2O | - |
Bothrops jararaca | dGMP + phosphate | - |
? | |
dGTP + H2O | - |
Bothrops jararaca | dGDP + phosphate | - |
? | |
GDP + H2O | - |
Bothrops jararaca | GMP + phosphate | - |
? | |
GTP + H2O | - |
Bothrops jararaca | GDP + phosphate | - |
? | |
IDP + H2O | - |
Bothrops jararaca | IMP + phosphate | - |
? | |
ITP + H2O | - |
Bothrops jararaca | IDP + phosphate | - |
? | |
additional information | the enzyme is not active upon AMP, GMP, dAMP, IMP, CMP, dGMP, dADP, TDP, TMP, UMP, dCMP, 3'-AMP, cAMP, and 4-nitrophenyl phosphate | Bothrops jararaca | ? | - |
? | |
additional information | the enzyme preferentially hydrolyzes nucleoside 5'-triphosphates over nucleoside 5'-diphosphates | Bothrops jararaca | ? | - |
? | |
p-nitrophenyl 5'-thymidine monophosphate + H2O | - |
Bothrops jararaca | 4-nitrophenol + TMP | - |
? | |
TTP + H2O | - |
Bothrops jararaca | TDP + phosphate | - |
? | |
UDP + H2O | - |
Bothrops jararaca | UMP + phosphate | - |
? | |
UTP + H2O | highest rate of hydrolysis | Bothrops jararaca | UDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NPP | - |
Bothrops jararaca |
nucleotide pyrophosphatase/phosphodiesterase | - |
Bothrops jararaca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44000 | - |
bis(4-nitrophenyl) phosphate | in 0.1 M glycine.NaOH, pH 8.9, at 37°C | Bothrops jararaca | |
960000 | - |
p-nitrophenyl 5'-thymidine monophosphate | in 0.1 M glycine.NaOH, pH 8.9, at 37°C | Bothrops jararaca |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme inhibits ADP-induced platelet aggregation, whereas thrombin-induced platelet aggregation is only slightly attenuated | Bothrops jararaca |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2000 | - |
bis(4-nitrophenyl) phosphate | in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca | |
11000000 | - |
S-5'-deoxyadenosylglutathione | in 0.1 M glycine-NaOH, pH 8.9, at 37°C | Bothrops jararaca |