Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, cloning in Escherichia coli TOP10 cells, and overexpression as His6-tagged protein in Escherichia coli strain BL21 (DE3)pLysS cells using pEXP5-CT/TOPO vector. Expression in Pichia pastoris strain X-33 | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Triticum aestivum | |
EDTA | activity is restorable by Zn2+ | Triticum aestivum | |
additional information | poor inhibition by imidazole at 2 mM | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Triticum aestivum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | contains a putative transmembrane domain | Triticum aestivum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | activates | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | D9YT79 | cf. EC 3.1.4.1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from wheat germs, recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Triticum aestivum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
188.2 | - |
purified recombinant enzyme, pH 8.7, 35°C | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-5'-TMP + H2O | - |
Triticum aestivum | 4-nitrophenol + 5'-TMP | - |
? | |
ADP-ribose + H2O | best substrate | Triticum aestivum | AMP + phosphoribose | - |
? | |
ATP + H2O | low activity | Triticum aestivum | ADP + phosphate | - |
? | |
CTP + H2O | low activity | Triticum aestivum | CDP + phosphate | - |
? | |
GTP + H2O | low activity | Triticum aestivum | GDP + phosphate | - |
? | |
NAD+ + H2O | - |
Triticum aestivum | NMN + AMP | - |
? | |
NADH + H2O | - |
Triticum aestivum | NMNH + AMP | - |
? | |
NADP+ + H2O | - |
Triticum aestivum | NMN + adenosine 2',5'-phosphate | - |
? | |
NADPH + H2O | high activity | Triticum aestivum | NMNH + adenosine 2',5'-phosphate | - |
? | |
TTP + H2O | low activity | Triticum aestivum | TDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
NPP | - |
Triticum aestivum |
nucleotide pyrophosphatase/phosphodiesterase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Triticum aestivum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified enzyme, pH 8.7, 30 min, stable, 75% activity remaining after 90 min | Triticum aestivum |
80 | - |
purified enzyme, pH 8.7, half-life is 5 min | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
12 | - |
- |
Triticum aestivum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | purified enzyme, 35°C, 30 min, over 75% activity remaining | Triticum aestivum |