Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano | - |
Homo sapiens | |
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate | - |
Homo sapiens | |
diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano | - |
Homo sapiens | |
additional information | diadenosine 5,5-(boranated)polyphosphonate analogues as selective nucleotide pyrophosphatase/phosphodiesterase inhibitors, synthesis, overview; diadenosine 5,5-(boranated)polyphosphonate analogues as selective nucleotide pyrophosphatase/phosphodiesterase inhibitors, synthesis, overview; diadenosine 5,5-(boranated)polyphosphonate analogues as selective nucleotide pyrophosphatase/phosphodiesterase inhibitors, synthesis, overview | Homo sapiens | |
P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-5'-TMP + H2O | Homo sapiens | hydrolysis of pnp-TMP at the cell surface of both osteocarcinoma and colon cancer cells | 4-nitrophenol + 5'-TMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | O14638 | NPP3 | - |
Homo sapiens | P22413 | NPP1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone cancer cell | - |
Homo sapiens | - |
colonic cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-5'-TMP + H2O | hydrolysis of pnp-TMP at the cell surface of both osteocarcinoma and colon cancer cells | Homo sapiens | 4-nitrophenol + 5'-TMP | - |
? | |
4-nitrophenyl-5'-TMP + H2O | a specific NPP substrate | Homo sapiens | 4-nitrophenol + 5'-TMP | - |
? | |
ATP + 2 H2O | - |
Homo sapiens | AMP + 2 phosphate | - |
? | |
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano + 2 H2O | low hydrolysis activity with the inhibitor as substrate | Homo sapiens | ? | - |
? | |
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate + 2 H2O | low hydrolysis activity with the inhibitor as substrate | Homo sapiens | ? | - |
? | |
diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano + 2 H2O | low hydrolysis activity with the inhibitor as substrate | Homo sapiens | ? | - |
? | |
P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate + 2 H2O | low hydrolysis activity with the inhibitor as substrate | Homo sapiens | ? | - |
? | |
P1,P5-bis(5'-adenosyl)pentaphosphate + 2 H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NPP | - |
Homo sapiens |
NPP1 | - |
Homo sapiens |
NPP2 | - |
Homo sapiens |
NPP3 | - |
Homo sapiens |
nucleotide pyrophosphatase/phosphodiesterase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano | with NPP1, pH 7.3, 37°C | Homo sapiens | |
0.013 | - |
P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate | with NPP1, pH 7.3, 37°C | Homo sapiens | |
0.02 | - |
diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano | with NPP1, pH 7.3, 37°C | Homo sapiens | |
0.051 | - |
di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate | with NPP1, pH 7.3, 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.013 | - |
with NPP1, pH 7.3, 37°C | Homo sapiens | di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano | |
0.033 | - |
with NPP1, pH 7.3, 37°C | Homo sapiens | P1,P4-bis(5'-adenosyl)-alpha,beta-gamma,delta-bismethylene-tetraphosphate | |
0.05 | - |
with NPP1, pH 7.3, 37°C | Homo sapiens | di-2'-deoxyadenosine 5',5''-P1,P5,alpha,beta-methylene-gamma,delta-methylene-tetraphosphate | |
0.063 | - |
with NPP1, pH 7.3, 37°C | Homo sapiens | diadenosine 5',5''-P1,P5,alpha,beta-methylene-delta,epsilon-methylene-pentaphosphate-gamma-borano |
General Information | Comment | Organism |
---|---|---|
physiological function | nucleotide pyrophosphatase/phosphodiesterases hydrolyze extracellular nucleotides and dinucleotides and thus control purinergic signaling. EnhancedNPP activity is implicated in health disorders such as osteoarthritis and cancer | Homo sapiens |