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Literature summary for 3.6.1.9 extracted from

  • Yano, T.; Funakoshi, I.; Yamashina, I.
    Purification and properties of nucleotide pyrophosphatase from human placenta (1985), J. Biochem., 98, 1097-1107.
    View publication on PubMed
Search for more literature for 3.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
2,3-dimercapto-1-propanol nearly complete inactivation at 3 mM Homo sapiens
2-mercaptoethanol 80% inhibition at 5 mM Homo sapiens
Cu2+ inhibition at 5 mM Homo sapiens
EDTA abolishes activity and makes enzyme unstable Homo sapiens
glycine
-
 Homo sapiens
Hg2+ inhibition at 5 mM Homo sapiens
Ni2+ inhibition at 5 mM Homo sapiens
Zn2+ inhibition at 5 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10% activation at 10 mM, 5 mM obligatory for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 4 * 130000, SDS-PAGE Homo sapiens
520000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Homo sapiens low activity AMP + phosphate
-
 ?
ATP + H2O Homo sapiens
-
 AMP + diphosphate
-
 ?
GDP + H2O Homo sapiens low activity GMP + phosphate
-
 ?
GDP-mannose + H2O Homo sapiens
-
 GMP + D-mannose-1-phosphate
-
 ?
NADH + H2O Homo sapiens
-
 NMNH + AMP
-
 ?
poly (ADP-ribose) + H2O Homo sapiens
-
 phosphoribosyladenosine 5'-phosphate + ?
-
 ?
polynucleotide + H2O Homo sapiens hardly degraded nucleoside 5'-phosphate + ?
-
 ?
UDP-alpha-D-galactose + H2O Homo sapiens
-
 UMP + alpha-D-galactose-1-phosphate
-
 ?
UDP-glucose + H2O Homo sapiens
-
 UMP + D-glucose-1-phosphate
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + H2O Homo sapiens
-
 N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + H2O Homo sapiens
-
 UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75 179
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-5'-ApU + H2O
-
 Homo sapiens adenosine + uridine 5'-phosphate
-
 ?
3'-5'-ApA + H2O
-
 Homo sapiens adenosine + adenosine 5'-phosphate
-
 ?
3'-5'-ApU + H2O
-
 Homo sapiens adenosine + uridine 5'-phosphate
-
 ?
3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Homo sapiens 3'-phosphoadenosine + phosphosulfate
-
 ?
adenosine 5'-phosphosulfate + H2O
-
 Homo sapiens adenosine + phosphosulfate
-
 ?
ADP + H2O low activity Homo sapiens AMP + phosphate
-
 ?
ATP + H2O
-
 Homo sapiens AMP + diphosphate
-
 ?
GDP + H2O low activity Homo sapiens GMP + phosphate
-
 ?
GDP-mannose + H2O
-
 Homo sapiens GMP + D-mannose-1-phosphate
-
 ?
NADH + H2O
-
 Homo sapiens NMNH + AMP
-
 ?
poly (ADP-ribose) + H2O
-
 Homo sapiens phosphoribosyladenosine 5'-phosphate + ?
-
 ?
polynucleotide + H2O hardly degraded Homo sapiens nucleoside 5'-phosphate + ?
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Homo sapiens thymidine 5'-monophosphate + p-nitrophenol
-
 ?
UDP-alpha-D-galactose + H2O
-
 Homo sapiens UMP + alpha-D-galactose-1-phosphate
-
 ?
UDP-glucose + H2O
-
 Homo sapiens UMP + D-glucose-1-phosphate
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + H2O
-
 Homo sapiens N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Homo sapiens UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 130000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NPPase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.6
-
-
 Homo sapiens