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Literature summary for 3.6.1.9 extracted from

  • Vollmayer, P.; Clair, T.; Goding, J.W.; Sano, K.; Servos, J.; Zimmermann, H.
    Hydrolysis of diadenosine polyphosphates by nucleotide pyrophosphatases/phosphodiesterases (2003), Eur. J. Biochem., 270, 2971-2978.
    View publication on PubMed
Search for more literature for 3.6.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS cells, NPP1 Homo sapiens
expression in COS cells, NPP2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue 0.1 mM, substrate Ap4A, strong inhibition, NPP1; 0.1 mM, substrate Ap4A, strong inhibition, NPP2 Homo sapiens
Cibacron blue 0.1 mM, substrate Ap4A, strong inhibition, NPP1 Rattus norvegicus
EDTA NPP1; NPP2 Homo sapiens
EDTA NPP3 Rattus norvegicus
heparan sulfate NPP1 Homo sapiens
heparin NPP1 Homo sapiens
additional information NPP3 is not affected by 0.1 mM suramin Rattus norvegicus
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid 0.1 mM, substrate p4A, 42% inhibition, NPP2; 0.1 mM, substrate p4A, about 85% inhibition, NPP1 Homo sapiens
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid 0.1 mM, substrate Ap4A, about 85% inhibition, NPP3 Rattus norvegicus
suramin 0.1 mM, substrate Ap4A, about 77% inhibition, NPP2; 0.1 mM, substrate p4A, about 70% inhibition, NPP1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 Ap3A pH 8.5, NPP1 Homo sapiens
0.008
-
 Ap3A pH 9.0, NPP2 Homo sapiens
0.0495
-
 Ap3A pH 8.5, NPP3 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane of COS cells, NPP1 Homo sapiens 16020
-
membrane of COS cells, NPP3 Rattus norvegicus 16020
-
soluble NPP2 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1 Homo sapiens
Ca2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2 Homo sapiens
Ca2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3 Rattus norvegicus
Mg2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1 Homo sapiens
Mg2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2 Homo sapiens
Mg2+ catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 NPP1
-
Homo sapiens
-
 NPP2
-
Rattus norvegicus P97675 NPP3, recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ap3A + H2O
-
 Homo sapiens ?
-
 ?
Ap3A + H2O
-
 Rattus norvegicus ?
-
 ?
diadenosine 5',5''-P1,P3-triphosphate + H2O NPP1 Homo sapiens 5'-ADP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P3-triphosphate + H2O NPP2 Homo sapiens 5'-ADP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P3-triphosphate + H2O NPP3 Rattus norvegicus 5'-ADP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O NPP1 Homo sapiens ATP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O NPP2 Homo sapiens ATP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O NPP3 Rattus norvegicus ATP + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O NPP1 Homo sapiens p4A + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O NPP2 Homo sapiens p4A + 5'-AMP
-
 ?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O NPP3 Rattus norvegicus p4A + 5'-AMP
-
 ?
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O NPP1 Homo sapiens GTP + 5'-GMP
-
 ?
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O NPP2 Homo sapiens GTP + 5'-GMP
-
 ?
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O NPP3 Rattus norvegicus GTP + 5'-GMP
-
 ?

Synonyms

Synonyms Comment Organism
NPP1
-
 Homo sapiens
NPP2
-
 Homo sapiens
NPP3
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 NPP1 Homo sapiens
8.5 9 NPP3 Rattus norvegicus
10
-
 NPP2 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.5 pH 7.4: about 10% of maximal activity, pH 8.5-9.0: maximal activity, NPP1 Homo sapiens
7.4 8.5 pH 7.4: about 30% of maximal activity, pH 8.5-9.0: maximal activity, NPP3 Rattus norvegicus
7.4 10 pH 7.4: about 15% of maximal activity, pH 10: maximal activity, NPP2 Homo sapiens