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Literature summary for 3.6.1.9 extracted from
- Purification and properties of a mouse liver plasma-membrane glycoprotein hydrolysing nucleotide pyrophosphate and phosphodiester bonds (1973), Biochem. J., 135, 819-826.
General Stability
| General Stability | Organism |
|---|---|
| slight loss of activity if repeated freezed and thawed | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong inhibition at 1 mM, partially reversed by Mg2+, complete reactivation at 10 mM Ca2+ | Mus musculus |  |
| EGTA | strong inhibition at 1 mM, partially reversed by Mg2+, complete reactivation at 10 mM Ca2+ | Mus musculus | |
| UDP-galactose | competitive inhibition, Ki: 29 mM | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
p-nitrophenyl deoxyTMP | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Mus musculus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2fold activation at 10 mM | Mus musculus | |
| Mg2+ | 2fold activation at 10 mM | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Mus musculus | - |
AMP + diphosphate | - |
? | |
| NAD+ + H2O | Mus musculus | - |
NMN + AMP | - |
? | |
| NADH + H2O | Mus musculus | - |
NMNH + AMP | - |
? | |
| UDP-alpha-D-galactose + H2O | Mus musculus | - |
UMP + alpha-D-galactose-1-phosphate | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | Mus musculus | - |
UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 5% neutral sugar content, glucosamine, sialic acid, glucose, galactose, mannose, fucose | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Mus musculus | AMP + diphosphate | - |
? | |
| NAD+ + H2O | - |
Mus musculus | NMN + AMP | - |
? | |
| NADH + H2O | - |
Mus musculus | NMNH + AMP | - |
? | |
| p-nitrophenyl-dTMP + H2O | Ca2+-dependent | Mus musculus | p-nitrophenol + dTMP | - |
? | |
| UDP-alpha-D-galactose + H2O | - |
Mus musculus | UMP + alpha-D-galactose-1-phosphate | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Mus musculus | UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 130000, SDS-PAGE | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
- |
Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29 | - |
UDP-galactose | competitive inhibition | Mus musculus | |
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