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Literature summary for 3.6.1.9 extracted from
- A reduced pyridine nucleotide pyrophosphatase (1957), J. Biol. Chem., 226, 427-437.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-ADP | 54% inhibition at 2 mM | Columba livia |  |
| 5'-AMP | 73% inhibition at2 mM | Columba livia | |
| adenosine 5'-sulfate | 46% inhibition at 2 mM | Columba livia | |
| ADP-ribose | 47% inhibition at 0.5 mM, competitive type of inhibition | Columba livia | |
| NADH | 14% inhibition at 2 mM | Columba livia | |
| NaF | 82% inhibition at 10 mM | Columba livia | |
| Sodium diphosphate | 66% inhibition at 25 mM | Columba livia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
NADH | reduced form | Columba livia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal stimulation at 6 mM | Columba livia | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | Columba livia | high activity | AMP + phosphoribose | - |
? | |
| diadenosine 5',5''-P1, P2-diphosphate + H2O | Columba livia | - |
AMP + AMP | - |
? | |
| FAD + H2O | Columba livia | - |
FMN + AMP | - |
? | |
| NADH + H2O | Columba livia | reduced and oxidized forms, split by two different pyrophosphatase in the crude extract, oxidized form not split by the purified enzyme | NMNH + AMP | - |
? | |
| NADH + H2O | Columba livia | alpha-isomer, reduced activity for both reduced and oxidized forms | NMNH + AMP | - |
? | |
| NADP+ + H2O | Columba livia | only reduced form hydrolysed | nicotinamide nucleotide + adenosine 2',5'-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Columba livia | - |
pigeon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Columba livia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Columba livia | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30-50% loss of activity in 5 days | Columba livia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | high activity | Columba livia | AMP + phosphoribose | - |
? | |
| diadenosine 5',5''-P1, P2-diphosphate + H2O | - |
Columba livia | AMP + AMP | - |
? | |
| diadenosine 5'-diphosphate + H2O | - |
Columba livia | adenosine 5'-monophosphate + ? | - |
? | |
| diphospho-3-acetylpyridine nucleotide + H2O | reduced form, 2fold higher activity than for NAD+ | Columba livia | ? | - |
? | |
| diphospho-deaminopyridine nucleotide + H2O | - |
Columba livia | ? | - |
? | |
| FAD + H2O | - |
Columba livia | FMN + AMP | - |
? | |
| NADH + H2O | reduced and oxidized forms, split by two different pyrophosphatase in the crude extract, oxidized form not split by the purified enzyme | Columba livia | NMNH + AMP | - |
? | |
| NADH + H2O | alpha-isomer, reduced activity for both reduced and oxidized forms | Columba livia | NMNH + AMP | - |
? | |
| NADP+ + H2O | only reduced form hydrolysed | Columba livia | nicotinamide nucleotide + adenosine 2',5'-bisphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphosphopyridine nucleotide pyrophosphatase | - |
Columba livia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Columba livia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
65-75% remaining activity | Columba livia |
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Release 2023.1 (January 2023)
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