Information on EC 3.5.1.11 - penicillin amidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.1.11
-
RECOMMENDED NAME
GeneOntology No.
penicillin amidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
penicillin + H2O = a carboxylate + 6-aminopenicillanate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
hydrolysis of peptide bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
Penicillin and cephalosporin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
penicillin amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9014-06-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
strain 14945
-
-
Manually annotated by BRENDA team
strain BM1
-
-
Manually annotated by BRENDA team
strain MARC-0103, isolated from rice starch
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-
Manually annotated by BRENDA team
strain NCIM 3425
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain WCFS1
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-
Manually annotated by BRENDA team
DSMZ 30186
-
-
Manually annotated by BRENDA team
Bro1 mutant strain
-
-
Manually annotated by BRENDA team
Pseudomonas melanogenum
-
-
-
Manually annotated by BRENDA team
Chainia
-
-
Manually annotated by BRENDA team
Chainia
-
-
Manually annotated by BRENDA team
gene pac
UniProt
Manually annotated by BRENDA team
PGA gene from a metagenomic library of an Antarctic sample
UniProt
Manually annotated by BRENDA team
TaxID 86473, growth on D-phenylglycine as sole source of nitrogen
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-methyl mandelate + H2O
?
show the reaction diagram
-
-
-
-
?
(R) -2-(4-hydroxyphenyl)glycine amide + H2O
(R) -2-(4-hydroxyphenyl)glycine + hydroxylamine
show the reaction diagram
-
-
-
?
(R)-2-phenylglycine amide + H2O
(R)-2-phenylglycine + hydroxylamine
show the reaction diagram
(R)-2-phenylglycine methyl ester + H2O
(R)-2-phenylglycine + methanol
show the reaction diagram
(R)-beta-phenylalanine + phenylacetamide
N-phenylacetyl-(R)-beta-phenylalanine + ?
show the reaction diagram
-
the acylation reaction is highly preferential for the acylation of (R)-beta-phenylalanine
-
-
?
(R)-mandelic acid methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
(R)-N-acetylphenylglycine + H2O
?
show the reaction diagram
-
S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
(R)-p-hydroxyphenylglycinamide + H2O
?
show the reaction diagram
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
(R)-phenylglycinamide + H2O
?
show the reaction diagram
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
(S)-N-acetylphenylglycine + H2O
?
show the reaction diagram
-
S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
(S)-p-hydroxyphenylglycinamide + H2O
?
show the reaction diagram
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
(S)-phenylglycinamide + H2O
?
show the reaction diagram
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5C to 45C
-
?
2-furylmethylpenicillin + H2O
6-aminopenicillanate + furan-2-yl-acetic acid
show the reaction diagram
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + phenoxyacetate
show the reaction diagram
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
?
show the reaction diagram
-
-
-
?
2-nitro-5-phenoxyacetamide benzoic acid + H2O
?
show the reaction diagram
-
-
-
?
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + ?
show the reaction diagram
-
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
show the reaction diagram
2-phenyl-N-(4-sulfamoylphenyl)acetamide + H2O
phenylacetate + 2-amino-4-sulfamoylphenol
show the reaction diagram
-
-
-
-
?
2-phenylacetamidobenzoic acid + H2O
phenylacetate + 2-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
2-thienylmethylpenicillin + H2O
6-aminopenicillanate + sulfo-(5-sulfothiophen-2-yl)-acetic acid
show the reaction diagram
-
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O
6-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O
?
show the reaction diagram
3-phenylacetamidobenzoic acid + H2O
phenylacetate + 3-aminobenzoic acid
show the reaction diagram
4-chloro-L-phenylalanine + phenylacetamide
N-[(4-chlorophenyl)acetyl]-L-tyrosine + NH3
show the reaction diagram
-
-
-
-
?
4-fluoro-L-phenylalanine + phenylacetamide
N-[(4-fluorophenyl)acetyl]-L-tyrosine + NH3
show the reaction diagram
-
-
-
-
?
4-hydroxyphenylacetamide + 6-aminopenicillanic acid
?
show the reaction diagram
4-methyl-L-phenylalanine + phenylacetamide
N-[(4-methylphenyl)acetyl]-L-tyrosine + NH3
show the reaction diagram
-
-
-
-
?
4-nitro-L-phenylalanine + phenylacetamide
N-[(4-nitrophenyl)acetyl]-L-tyrosine + NH3
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
4-phenylacetamidobenzoic acid + H2O
phenylacetate + 4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
5-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetate + 3-amino-5-nitro-benzoic acid
show the reaction diagram
-
-
-
-
?
5-[[(2R)-2-amino-2-phenylethanoyl]amino]-2-nitrobenzoic acid + H2O
5-amino-2-nitrobenzoic acid + (R)-2-phenylglycine
show the reaction diagram
-
-
-
?
6-aminopenicillanate + phenylacetic acid
penicillin G + H2O
show the reaction diagram
6-aminopenicillanic acid + methyl n-hexanoate
dihydropenicillin F + methanol
show the reaction diagram
-
-
-
-
?
6-aminopenicillanic acid + methyl n-octanoate
penicillin K + methanol
show the reaction diagram
-
-
-
-
?
6-aminopenicillanic acid + p-hydroxyphenylglycine methyl ester
amoxicillin + methanol
show the reaction diagram
-
-
-
-
?
6-aminopenicillanic acid + phenoxyacetic acid
penicillin V + H2O
show the reaction diagram
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
show the reaction diagram
6-nitro-3-(phenylacetamido)benzoic acid + H2O
3-amino-6-nitrobenzoic acid + ?
show the reaction diagram
-
-
-
-
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
show the reaction diagram
-
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
show the reaction diagram
6-nitro-3-phenylacetamido-benzoic acid + H2O
?
show the reaction diagram
6-nitro-3-phenylacetamidobenzoic acid + H2O
?
show the reaction diagram
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
show the reaction diagram
7-amino-3-deacetoxycephalosporanic acid + phenylglycine methyl ester
cephalexin + ?
show the reaction diagram
-
-
-
-
?
7-amino-3-propenyl-cephalosporanic acid + 4-hydroxy-D-phenylglycine methyl ester
cefprozil + ?
show the reaction diagram
-
-
-
-
?
7-amino-deacetoxycephalosporanic acid + phenylacetic acid
deacetoxycephalosporin G + H2O
show the reaction diagram
7-amino-desacetoxycephalosporanic acid + D-alpha-phenylglycine methylester
?
show the reaction diagram
-
acylating activity
-
-
?
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide
cephalexin
show the reaction diagram
-
high reactant concentrations
-
-
r
7-aminocephalosporanic acid + (R)-mandelic acid
7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-D3-cephem-4-carboxylic acid
show the reaction diagram
-
-
-
-
-
7-aminocephalosporanic acid + D-alpha-phenylglycine methylester
?
show the reaction diagram
-
acylating activity
-
-
?
7-aminodeacetoxycephalosporanic acid + (R)-2-phenylglycine methylester
cephalexin + ?
show the reaction diagram
-
-
-
-
?
7-aminodeacetoxycephalosporanic acid + 2-benzoxazolon-3-yl-acetic acid methyl ester
?
show the reaction diagram
-
-
-
?
7-aminodeacetoxycephalosporanic acid + phenoxyacetic acid
deacetoxycephalosporin V + H2O
show the reaction diagram
-
-
-
-
?
7-aminodeacetoxycephalosporanic acid + phenylacetic acid methyl ester
?
show the reaction diagram
-
-
-
?
7-phenylacetamidoacetoxycephalosporanic acid + H2O
?
show the reaction diagram
-
-
-
-
?
allopurinol + vinyl acetate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl acetate
show the reaction diagram
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allopurinol + vinyl decanoate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl decanoate
show the reaction diagram
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allopurinol + vinyl pentanoate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl pentanoate
show the reaction diagram
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allylmercaptomethylpenicillin + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
amoxicillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
show the reaction diagram
-
-
-
-
r
amoxillin + H2O
D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
show the reaction diagram
amoxycillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
show the reaction diagram
-
-
-
?
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
show the reaction diagram
ampicillin + H2O
6-aminopenicillanic acid + (R)-2-phenylglycine
show the reaction diagram
ampicillin + H2O
?
show the reaction diagram
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
show the reaction diagram
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
show the reaction diagram
benzylpenicilloic acid + H2O
?
show the reaction diagram
beta-(alpha-toluylamido)-propionic acid + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
capsaicin + H2O
?
show the reaction diagram
-
-
-
?
carbenicillin + H2O
phenylpropanedioate + 6-aminopenicillate
show the reaction diagram
-
-
-
?
cefadroxil + H2O
?
show the reaction diagram
cefamandole + H2O
(6R,7R)-7-amino-3-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-hydroxy(phenyl)acetic acid
show the reaction diagram
-
-
-
-
?
cephadrine + H2O
?
show the reaction diagram
Pseudomonas melanogenum
-
-
-
-
?
cephalexin + H2O
(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
show the reaction diagram
-
-
-
-
?
cephalexin + H2O
7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + aminophenylacetic acid
show the reaction diagram
cephalexin + H2O
7-aminodesacetoxycephalosporanic acid + aminophenylacetic acid
show the reaction diagram
cephalexin + H2O
D-phenylglycine + 7-aminodesacetoxycephalosporanic acid
show the reaction diagram
-
-
-
-
r
cephaloglycin + H2O
(6R,7R)-7-amino-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-amino(phenyl)acetic acid
show the reaction diagram
Pseudomonas melanogenum
-
-
-
-
?
cephaloglycin + H2O
?
show the reaction diagram
-
-
-
-
?
cephaloridine + H2O
(6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
show the reaction diagram
cephaloridine + H2O
thiophen-2-ylacetic acid + (6R,7R)-7-amino-8-oxo-3-(pyridinium-1-yl-methyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
show the reaction diagram
-
-
-
?
cephalosporanic acid V + H2O
?
show the reaction diagram
-
-
-
-
?
cephalosporin + H2O
7-aminocephalosporanic acid + an amino acid
show the reaction diagram
cephalosporin G + H2O
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide
show the reaction diagram
-
-
-
-
?
cephalosporin G + H2O
7-aminocephalosporanic acid + phenylacetic acid
show the reaction diagram
-
-
-
-
r
cephalosporin G + H2O
phenylacetic acid + 7-aminodeacetoxycephalosporanate
show the reaction diagram
-
-
-
-
?
cephalothin + H2O
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
show the reaction diagram
cephalothin + H2O
thiophen-2-ylacetic acid + (6R,7R)-3-acetoxymethyl-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
show the reaction diagram
-
-
-
?
cephradine + H2O
(2R)-amino(cyclohexa-1,4-dien-1-yl)acetic acid + (6R,7R)-7-amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
show the reaction diagram
-
-
-
-
?
D,L-N-phenylacetylalanine + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
D-(-)-alpha-aminophenylacetic acid 4-nitroanilide + H2O
D-(-)-alpha-aminophenylacetic acid + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
show the reaction diagram
D-2-nitro-5[(phenylglycyl)amino]benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
amoxicillin + NH3
show the reaction diagram
D-alpha-aminobenzylpenicillin + H2O
6-aminopenicillanate + DL-alpha-aminophenylacetic acid
show the reaction diagram
-
-
-
?
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + H2O
show the reaction diagram
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + NH3
show the reaction diagram
-
-
-
-
r
D-phenylglycine amide + 7-aminodeacetoxycephalosporanic acid
cephalexin + ?
show the reaction diagram
-
-
-
-
?
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin
show the reaction diagram
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin + NH3
show the reaction diagram
-
-
-
-
r
D-phenylglycine amide + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
cephaclor + ?
show the reaction diagram
-
-
-
-
?
D-phenylglycine amide + H2O
D-phenylglycine + NH3
show the reaction diagram
-
-
-
-
?
D-phenylglycine methyl ester + 6-aminopenicillanic acid
methanol + ampicillin
show the reaction diagram
-
-
-
r
D-phenylglycine methyl ester + 6-aminopenicillic acid
ampicillin + methanol
show the reaction diagram
-
high ratio of synthesis to hydrolysis at up to 200 mM 6-aminopenicillic acid and 500 mM D-phenylglycine methyl ester at 25C and pH 6.5. When concentration of 6-aminopenicillic acid reaches saturation, rate of hydrolysis tends toward zero
-
-
r
D-phenylglycine methyl ester + 7-aminodeacetoxycephalosporanic acid
cephalexin + ?
show the reaction diagram
-
-
-
-
?
D-phenylglycine methyl ester + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
cephaclor + ?
show the reaction diagram
-
-
-
-
?
delta2-pentenylpenicillin + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
diethyl phenylmalonate + H2O
(+)-ethyl phenylmalonate + ethanol
show the reaction diagram
-
the enantiomeric excess is higher than 98%, producing mainly the (+)-ethyl phenylmalonate ester
-
-
?
dimethyl phenylmalonate + H2O
(+)-methyl phenylmalonate + methanol
show the reaction diagram
-
dimethyl phenylmalonate is fully hydrolyzed to methylphenylmalonate in only 2 h, but even after 10 h phenylmalonic acid is not detected. The enantiomeric excess is higher than 98%, producing mainly the (+)-methyl phenylmalonate ester
-
-
?
DL-alpha-hydroxybenzylpenicillin + H2O
6-aminopenicillanate + DL-alpha-hydroxyphenylacetic acid
show the reaction diagram
-
-
-
?
ethyl 4-[(phenylacetyl)amino] benzoate + H2O
phenylacetate + 4-amino-ethylbenzoate
show the reaction diagram
-
-
-
-
?
ethylthiomethylpenicillin + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
glutarate + 7-aminocephalosporanic acid
show the reaction diagram
-
-
-
-
?
hydroxyphenylglycineamide + 7-aminodeacetoxycephalosporanic acid
cefadroxil + NH3
show the reaction diagram
-
-
-
-
-
isobutoxymethylpenicillin + H2O
6-aminopenicillanate + carbonic acid isopropyl ester
show the reaction diagram
-
-
-
-
?
L-phenylalanine + (2S)-2-amino-2-phenylethanamide
N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine + NH3
show the reaction diagram
-
-
-
-
?
L-phenylalanine + (2S)-2-hydroxy-2-phenylethanamide
N-[(2S)-2-hydroxy-2-phenylacetyl]-L-phenylalanine + NH3
show the reaction diagram
-
-
-
-
?
L-phenylalanine + 2-(4-hydroxyphenyl)acetamide
N-[(4-hydroxyphenyl)acetyl]-L-phenylalanine + NH3
show the reaction diagram
-
-
-
-
?
L-phenylalanine + methyl (2S)-hydroxy(4-hydroxyphenyl)ethanoate
N-[(2S)-2-hydroxy-2-phenylacetyl]-L-phenylalanine + methanol
show the reaction diagram
-
-
-
-
?
L-phenylalanine + methyl (4-hydroxyphenyl)acetate
N-[(4-hydroxyphenyl)acetyl]-L-phenylalanine + methanol
show the reaction diagram
-
-
-
-
?
L-phenylalanine + methyl phenylacetate
N-phenylacetyl-L-phenylalanine + methanol
show the reaction diagram
-
-
-
-
?
L-phenylalanine + N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine
N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine + methanol
show the reaction diagram
-
-
-
-
?
L-phenylalanine + phenylacetamide
N-phenylacetyl-L-phenylalanine + NH3
show the reaction diagram
-
-
-
-
?
L-tyrosine + phenylacetamide
N-(phenylacetyl)-L-tyrosine + NH3
show the reaction diagram
-
-
-
-
?
N,2-diphenylacetamide + H2O
phenylacetate + ?
show the reaction diagram
-
-
-
-
?
N-(4-acetylphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-acetylphenol
show the reaction diagram
-
-
-
-
?
N-(4-bromophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-bromophenol
show the reaction diagram
-
-
-
-
?
N-(4-cyanophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-cyanophenol
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-methoxyphenol
show the reaction diagram
-
-
-
-
?
N-(4-methylphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-methylphenol
show the reaction diagram
-
-
-
-
?
N-(4-nitrophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-nitrophenol
show the reaction diagram
-
-
-
-
?
N-(5-nitro-2-pyridyl)-phenylacetamide + H2O
phenylacetate + 5-nitropyridine
show the reaction diagram
-
-
-
-
?
N-(phenylacetyl)glycine + H2O
phenylacetic acid + glycine
show the reaction diagram
-
-
-
?
N-methylphenylacetamide + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
N-phenylacetyl-Asp + H2O
phenylacetic acid + Asp
show the reaction diagram
-
-
-
?
N-phenylacetyl-Asp-Phe methyl ester + H2O
phenylacetic acid + Asp-Phe methyl ester
show the reaction diagram
-
-
-
?
N-phenylacetyl-DL-leucine + H2O
?
show the reaction diagram
-
-
-
-
?
N-phenylacetyl-DL-tert-leucine + H2O
L-tert-leucine + phenylacetate
show the reaction diagram
-
enantioselective hydrolysis
-
-
?
N-phenylacetyl-Glu + H2O
phenylacetic acid + Glu
show the reaction diagram
-
-
-
?
N-phenylacetyl-L-alpha-aminophenylacetic acid + H2O
?
show the reaction diagram
-
-
-
-
?
N-phenylacetyl-Leu + H2O
phenylacetic acid + Leu
show the reaction diagram
-
-
-
?
N-phenylacetyl-Phe + H2O
phenylacetic acid + Phe
show the reaction diagram
-
-
-
?
N-phenylacetylglycine + H2O
?
show the reaction diagram
-
-
-
-
?
n-propoxymethylpenicillin + H2O
6-aminopenicillanate + carbonic acid monopropyl ester
show the reaction diagram
-
-
-
-
?
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-(methylsulfonyl)phenol
show the reaction diagram
-
-
-
-
?
N2-phenylacetyl-2'-deoxyguanosine + H2O
phenylacetic acid + 2'-deoxyguanosine
show the reaction diagram
N6-phenylacetyl-2'-deoxyadenosine + H2O
phenylacetic acid + 2'-deoxyadenosine
show the reaction diagram
p-hydroxybenzylpenicillin + H2O
6-aminopenicillanate + p-hydroxyphenylacetic acid
show the reaction diagram
-
-
-
?
penicillin + H2O
a carboxylate + 6-aminopenicillanate
show the reaction diagram
-
-
-
?
penicillin dihydroF + H2O
6-aminopenicillanic acid + hexanoic acid
show the reaction diagram
-
-
-
-
?
penicillin dihydroF + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin F + H2O
6-aminopenicillanic acid + (3E)-hex-3-enoic acid
show the reaction diagram
-
-
-
-
?
penicillin F + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
show the reaction diagram
penicillin G + H2O
6-aminopenicillanic acid + phenyl acetic acid
show the reaction diagram
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
show the reaction diagram
penicillin G + H2O
phenyl acetic acid + 6-aminopenicillanate
show the reaction diagram
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
show the reaction diagram
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
show the reaction diagram
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanic acid
show the reaction diagram
-
-
-
-
?
penicillin K + H2O
6-aminopenicillanic acid + octanoic acid
show the reaction diagram
-
-
-
-
?
penicillin K + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
show the reaction diagram
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
show the reaction diagram
penicillin V + H2O
a carboxylate + 6-aminopenicillanate
show the reaction diagram
penicillin V + H2O
phenoxyacetate + 6-aminopenicillate
show the reaction diagram
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
show the reaction diagram
-
-
-
?
penicillin V + H2O
phenoxyacetic acid + 6-aminopenicillanate
show the reaction diagram
-
-
-
-
?
penicillin V sulfoxide + H2O
?
show the reaction diagram
-
-
-
-
?
penicillin-G + H2O
6-aminopenicillanic acid + phenylacetic acid
show the reaction diagram
phenoxyethylpenicillin + H2O
3-phenoxypropanoic acid + 6-aminopenicillanic acid
show the reaction diagram
-
poor substrate
-
-
?
phenylacetamide + 6-aminopenicillanic acid
?
show the reaction diagram
phenylacetamide + H2O
?
show the reaction diagram
phenylacetamide + H2O
NH3 + ?
show the reaction diagram
-
poor substrate
-
-
?
phenylacetamide + H2O
phenylacetate + NH3
show the reaction diagram
-
-
-
-
?
phenylacetate 4-nitroanilide + H2O
phenylacetic acid + 4-nitrolaniline
show the reaction diagram
-
-
-
-
?
phenylacetic acid + 6-aminopenicillanic acid
penicillin G
show the reaction diagram
phenylacetic hydrazide + H2O
phenylacetate + hydrazine
show the reaction diagram
-
-
-
-
?
phenylacetyl-2-naphthylamide + H2O
phenylacetate + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
phenylacetyl-4-aminobenzoic acid
4-aminobenzoic acid + phenylacetic acid
show the reaction diagram
-
colometric assay
-
?
phenylacetyl-7-amido-4-methylcoumarin + H2O
phenylacetate + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
phenylacetyl-anthranilic acid
1-naphthylamine + phenylacetic acid
show the reaction diagram
-
colometric assay
-
?
phenylacetyl-Gly + H2O
phenylacetic acid + glycine
show the reaction diagram
-
-
-
?
phenylacetyl-L-asparagine + H2O
L-asparagine + phenylacetic acid
show the reaction diagram
-
-
-
?
phenylacetyl-L-leucine + H2O
?
show the reaction diagram
-
-
-
-
?
phenylacetylaspartame + H2O
phenylacetic acid + aspartame
show the reaction diagram
-
-
-
?
phenylacetylglycine + 6-aminopenicillanic acid
penicillin G + glycine
show the reaction diagram
phenylglycine methyl ester + aminopenicillanic acid
ampicillin + methanol
show the reaction diagram
-
-
-
-
?
phenylglycineamide + 7-aminodeacetoxycephalosporanic acid
cefaclor + NH3
show the reaction diagram
-
-
-
-
-
phenylglycinemethylester + 7-aminodeacetoxymethyl-3-chlorocephalosporanic acid
cephalexin + methanol
show the reaction diagram
-
-
-
-
-
vanillylamine + methyl laurate
6-lauroyl-vanillylamide + methanol
show the reaction diagram
-
-
-
-
?
vanillylamine + methyl myristate
6-myristoyl-vinillylamide + methanol
show the reaction diagram
-
-
-
-
?
vanillylamine + methyl n-octanoate
6-octanoyl-vanillylamide + methanol
show the reaction diagram
-
-
-
-
?
[(2R,3S),(2S,3R)]-cis-3-amino-azetidinone + phenoxy-acetic acid methyl ester
(2R,3S)-beta-lactam intermediate + methanol
show the reaction diagram
-
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
show the reaction diagram
penicillin + H2O
a carboxylate + 6-aminopenicillanate
show the reaction diagram
Q60136
-
-
-
?
penicillin dihydroF + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin F + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenyl acetic acid
show the reaction diagram
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
show the reaction diagram
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
show the reaction diagram
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
show the reaction diagram
penicillin K + H2O
?
show the reaction diagram
-
substrate of immobilized enzyme
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
show the reaction diagram
Q72G76
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
show the reaction diagram
-
substrate of soluble and immobilized enzyme
-
-
?
penicillin V + H2O
a carboxylate + 6-aminopenicillanate
show the reaction diagram
-
-
-
-
?
penicillin V + H2O
phenoxyacetic acid + 6-aminopenicillanate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
catalytically active enzyme contains one tightly bound Ca2+
K+
activates 87.8% at 1 mM
Mg2+
activates 46.0% at 1 mM
Zinc
-
enzyme contains 2 zinc atoms per molecule
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-alpha-ethylphenylacetic acid
-
-
(R)-alpha-hydroxyphenylacetic acid
-
-
(R)-alpha-methylphenylacetic acid
-
-
(R)-phenylglycinamide
-
-
-
(R)-phenylglycine
-
-
(S)-alpha-ethylphenylacetic acid
-
-
(S)-alpha-hydroxyphenylacetic acid
-
-
(S)-alpha-methylphenylacetic acid
-
-
2,3-Butanedione
-
50 mM, 78% loss of activity, benzylpenicillin or phenylacetate protects
2-hydroxy 5-nitrobenzylbromide
-
-
2-thienylacetic acid
-
competitive inhibitor
6-aminopenicillanate
-
product inhibition
6-aminopenicillanic acid
7-amino-3-desacetoxycephalosporanic acid
-
non-competitive inhibitor
8-hydroxyquinoline
-
complexed with zinc, reactivation by addition of ZnSO4, MnSO4, MgSO4, CoSO4, FeSO4
acetone
-
upon permeabilization
benzyl penicillin
-
-
Benzylisocyanate
-
-
Ca2+
inhibits 15.1% at 1 mM
cephalosporin C
65% inhibition; 65% inhibition at 1 mM
cephalosporin G
79% inhibition; 79% inhibition at 1 mM
deoxycholic acid
slight inhibition
diethyldicarbonate
Pseudomonas melanogenum
-
-
dimethylformamide
inhibits 10.5% at 10% and 33.2% at 40%
EDTA
inhibits 80.4% at 10 mM
Fe3+
inhibits 96.0% at 1 mM
glycodeoxycholic acid
slight inhibition
Hg2+
Pseudomonas melanogenum
-
decreases enzyme activity to 50%
N-bromosuccinamide
-
0.1 mM, 70% loss of activity
N-bromosuccinimide
phenoxyacetic acid
phenylacetamide
-
-
phenylacetic acid
Phenylglyoxal
-
20 mM, 82% loss of activity, benzylpenicillin or phenylacetate protects
phenylmethanesulfonic acid N-hydroxysuccinimide ester
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
Phenylmethylsulfonyl azide
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
Phenylmethylsulfonyl chloride
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
phenylmethylsulfonyl fluoride
Phenylmethylsulfonylfluoride
-
-
PMSF
-
irreversible, used for active site titrations of the cross-linked enzyme aggregates
Pyridine
-
upon permeabilization
SDS
inhibits 56.0% at 2%
Sr2+
inhibits 95.4% at 1 mM
taurodeoxycholic acid
slight inhibition
Urea
inhibits 95.7% at 8 M, no inhibition at 4 M
[bmim]dca
-
the enzyme activity in 25% [bmim]dca is 1.6fold than activity in water. The enzyme activity is decreased in higher concentration of [bmim]dca
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chloroform
-
cell-bound PVA activity shows an eightfold increase upon treatment with chloroform at 0.005 ml/mg dry biomass for 10 min upon permeabilization
Dichloromethane
-
enhances the enzyme activity up to 2fold upon permeabilization
Diethyl ether
-
cell-bound PVA activity shows an eightfold increase upon treatment with diethylether at 0.010 ml/mg dry biomass for 45 min upon permeabilization
ethyl acetate
-
enhances the enzyme activity up to 4fold upon permeabilization
hexane
-
enhances the enzyme activity up to 2fold upon permeabilization
Toluene
-
enhances the enzyme activity up to 6fold upon permeabilization
[bmim]dca
-
the enzyme activity in 25% [bmim]dca is 1.6fold than activity in water. The enzyme activity is decreased in higher concentration of [bmim]dca
additional information
-
addition of chemically synthesized fragments of the pro-peptide to purified mature enzyme increases its specific activity up to 2.3fold
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
2-benzoxazolon-3-yl-acetic acid methyl ester
-
pH 6.8, 25C
0.63
2-nitro-5-(phenoxyacetamido)-benzoic acid
pH 6.6, 40C
1.6
2-nitro-5-phenoxyacetamide benzoic acid
-
0.00614 - 0.03
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
0.0005 - 0.797
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
0.147
2-phenyl-N-(4-sulfamoylphenyl)acetamide
-
pH 7.0, 25C
0.00063
2-phenylacetamidobenzoic acid
-
25C, pH 7.0, 10% dimethylsulfoxide
0.0004
3-phenylacetamidobenzoic acid
-
25C, pH 7.0, 10% dimethylsulfoxide
0.027 - 0.114
4-Hydroxyphenylacetamide
0.0003
4-phenylacetamidobenzoic acid
-
25C, pH 7.0, 10% dimethylsulfoxide
0.07 - 0.23
5-nitro-3-[(phenylacetyl)amino]benzoic acid
0.0083
6-nitro-3-(phenylacetamido)-benzoic acid
pH 7.4, 37C, recombinant enzyme
0.0176
6-nitro-3-phenylacetamide benzoic acid
-
pH 7.5, 37C
0.012 - 0.02
6-nitro-3-phenylacetamidobenzoic acid
0.000015 - 0.027
6-nitro-3-[(phenylacetyl)amino]benzoic acid
0.01
7-phenylacetamidodeacetoxycephalosporanic acid
-
-
0.399 - 1.07
amoxicillin
0.105
amoxycillin
pH 7.4, 37C, recombinant enzyme
0.0944 - 5.2
ampicillin
0.0031 - 4.5
benzylpenicillin
2
benzylpenicilloic acid
-
-
0.0519
carbenicillin
pH 7.4, 37C, recombinant enzyme
0.284 - 0.646
cefadroxil
1.79
cephadrine
Pseudomonas melanogenum
-
-
0.0255 - 2.1
cephalexin
1.96
cephaloglycin
Pseudomonas melanogenum
-
-
0.0164 - 0.1
Cephaloridine
0.0156 - 0.042
Cephalothin
3.2
D-(-)-alpha-aminophenylacetic acid p-nitroanilide
-
-
0.646 - 1.3
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid
9.1 - 12.2
D-4-hydroxyphenylglycine amide
2 - 30
D-phenylglycine amide
0.153
ethyl 4-[(phenylacetyl)amino] benzoate
-
pH 7.0, 25C
0.045
ethylphenylacetate
-
-
0.137
N,2-diphenylacetamide
-
pH 7.0, 25C
0.23
N-(4-acetylphenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.171
N-(4-bromophenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.16
N-(4-cyanophenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.162
N-(4-methoxyphenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.121
N-(4-methylphenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.095
N-(4-nitrophenyl)-2-phenylacetamide
-
pH 7.0, 25C
0.00051
N-(5-nitro-2-pyridyl)-phenylacetamide
-
25C, pH 7.0, 10% dimethylsulfoxide
0.159
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
-
pH 7.0, 25C
0.8
N2-phenylacetyl-2'-deoxyguanosine
-
pH 7.8, 25C
0.46
N6-phenylacetyl-2'-deoxyadenosine
-
pH 7.8, 25C
0.031
p-nitrophenylphenylacetate
-
-
5.6
penicillin dihydroF
-
pH 8.0, 45C
15.1
penicillin F
-
pH 8.0, 45C
0.00001 - 164
penicillin G
0.05 - 1
penicillin K
0.0393 - 40
Penicillin V
0.0002 - 0.156
phenylacetamide
0.0004
phenylacetate 4-nitroanilide
-
25C, pH 7.0, 10% dimethylsulfoxide
0.097
phenylacetate p-nitroanilide
-
-
0.0002
phenylacetyl-2-naphthylamide
-
25C, pH 7.0, 10% dimethylsulfoxide
0.4
phenylacetyl-3-aminobenzoic acid
-
-
0.18
phenylacetyl-4-aminobenzoic acid
-
-
0.000012
phenylacetyl-7-amido-4-methylcoumarin
-
25C, pH 7.0, 10% dimethylsulfoxide
0.95
phenylacetyl-anthranilic acid
-
-
5.2
phenylacetyl-Gly
-
pH 7.8, 25C
0.67
phenylacetylaspartame
-
pH 7.8, 25C
0.08
phenylacetylglycine
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
(R) -2-(4-hydroxyphenyl)glycine amide
Escherichia coli
-
-
1.01 - 6.08
(S)-N-acetylphenylglycine
0.1
2-benzoxazolon-3-yl-acetic acid methyl ester
Escherichia coli
-
pH 6.8, 25C
106.4
2-nitro-5-phenoxyacetamide benzoic acid
Streptomyces mobaraensis
Q0R3W5
-
0.204 - 15
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
18
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
Escherichia coli
-
pH 7.0, 30C, analysis of complex kinetic parameters alpha, beta, gamma
40.03
2-phenyl-N-(4-sulfamoylphenyl)acetamide
Alcaligenes faecalis
-
pH 7.0, 25C
2.3
2-phenylacetamidobenzoic acid
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
2.5
3-phenylacetamidobenzoic acid
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
29 - 47
4-Hydroxyphenylacetamide
170
4-nitrophenylacetate
Escherichia coli
-
-
3
4-phenylacetamidobenzoic acid
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
0.9 - 1.2
5-nitro-3-[(phenylacetyl)amino]benzoic acid
58.3
6-nitro-3-(phenylacetamido)-benzoic acid
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
20 - 82
6-nitro-3-phenylacetamidobenzoic acid
16 - 68.8
6-nitro-3-[(phenylacetyl)amino]benzoic acid
50
7-phenylacetamidoacetoxycephalosporanic acid
Escherichia coli
-
-
15 - 17
amoxicillin
56.2
amoxycillin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
11 - 49.5
ampicillin
21 - 190
benzylpenicillin
40
benzylpenicilloic acid
Escherichia coli
-
-
9.5
capsaicin
Streptomyces mobaraensis
Q0R3W5
-
63.7
carbenicillin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
13 - 32
cefadroxil
20 - 54
cephalexin
33 - 66.9
Cephaloridine
25 - 78.3
Cephalothin
0.54
D-(-)-alpha-aminophenylacetic acid 4-nitroanilide
Escherichia coli
-
-
12 - 14
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid
2 - 16
D-4-hydroxyphenylglycine amide
2.7 - 57
D-phenylglycine amide
41.05
ethyl 4-[(phenylacetyl)amino] benzoate
Alcaligenes faecalis
-
pH 7.0, 25C
170
ethylphenylacetate
Escherichia coli
-
-
49.17
N,2-diphenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
35.94
N-(4-acetylphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
43.07
N-(4-bromophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
33.84
N-(4-cyanophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
33.81
N-(4-methoxyphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
37.93
N-(4-methylphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
15.14
N-(4-nitrophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
7.8
N-(5-nitro-2-pyridyl)-phenylacetamide
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
12000
N-phenylacetyl-Glu
Escherichia coli
-
-
29.49
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
18
N2-phenylacetyl-2'-deoxyguanosine
Escherichia coli
-
pH 7.8, 25C
2
N6-phenylacetyl-2'-deoxyadenosine
Escherichia coli
-
pH 7.8, 25C
4.2
penicillin dihydroF
Actinoplanes utahensis
-
pH 8.0, 45C
1.9
penicillin F
Actinoplanes utahensis
-
pH 8.0, 45C
0.02 - 72.7
penicillin G
0.32 - 33.3
penicillin K
48.4 - 270.8
Penicillin V
23 - 50
phenylacetamide
20 - 55
phenylacetate 4-nitroanilide
1.9
phenylacetyl-2-naphthylamide
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
3.2
phenylacetyl-7-amido-4-methylcoumarin
Escherichia coli
-
25C, pH 7.0, 10% dimethylsulfoxide
47
phenylacetyl-Gly
Escherichia coli
-
pH 7.8, 25C
35
phenylacetylaspartame
Escherichia coli
-
pH 7.8, 25C
additional information
additional information
Escherichia coli
-
temperature dependence of turnover-numer for R- and S-p-hydroxyphenylglycineamide
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20 - 500
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
12148
272
2-phenyl-N-(4-sulfamoylphenyl)acetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41347
7024
6-nitro-3-(phenylacetamido)-benzoic acid
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
206932
534.2
amoxycillin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
13087
524.4
ampicillin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
191
1227
carbenicillin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
1436
1341
cephalexin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
1026
4079
Cephaloridine
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
591
5019
Cephalothin
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
354
268
ethyl 4-[(phenylacetyl)amino] benzoate
Alcaligenes faecalis
-
pH 7.0, 25C
41349
360
N,2-diphenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41351
156
N-(4-acetylphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41348
250
N-(4-bromophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41350
212
N-(4-cyanophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41346
209
N-(4-methoxyphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41353
314
N-(4-methylphenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41352
159
N-(4-nitrophenyl)-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41344
185
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
Alcaligenes faecalis
-
pH 7.0, 25C
41345
0.0005 - 13960
penicillin G
181
3.6 - 18.2
penicillin K
10119
1232
Penicillin V
uncultured bacterium
D6R1Q1
pH 7.4, 37C, recombinant enzyme
2755