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Literature summary for 3.5.1.11 extracted from

  • Kasche, V.; Galunsky, B.; Nurk, E.; Piotraschke, E.; Rieks, A.
    The dependency of the stereoselectivity of penicillin amidases – enzymes with R-specific S1 and S-specific S'1-subsites – on temperature and primary structure (1996), Biotechnol. Lett., 18, 455-460.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information temperature dependence of Km-value for (R)- and (S)p-hydroxyphenylglycineamide Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-N-acetylphenylglycine + H2O S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?
(R)-p-hydroxyphenylglycinamide + H2O R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?
(R)-phenylglycinamide + H2O R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?
(S)-N-acetylphenylglycine + H2O S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?
(S)-p-hydroxyphenylglycinamide + H2O R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?
(S)-phenylglycinamide + H2O R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C Escherichia coli ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information temperature dependence of turnover-numer for R- and S-p-hydroxyphenylglycineamide Escherichia coli
1.01
-
(S)-N-acetylphenylglycine pH 7.5 Escherichia coli
6.08
-
(S)-N-acetylphenylglycine pH 7.5 Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli proterolytically processed form
-
7