Cloned (Comment) | Organism |
---|---|
expression of functional wild-type and mutant enzymes in Pseudomonas aeruginosa PAO1 and PAO1DELTAtatABC strains using the pMMB67EH shuttle vector, the mutant a TatProPGA hybrid only shows an active site, when the transformed bacterium is grown at 25°C or lower temperatures. Processing of recombinant PGA can also occur in the cytoplasm of Pseudomonas aeruginosa. The extracellular localization of the TatProPGA hybrid is not dependent on the tatABC-genes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion protein, consisting of Pseudomonas Sec- or Tat-specific signal peptides, the elastase propeptide and the mature penicillin G acylase, termed a TatProPGA hybrid. the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein | Escherichia coli | 5829 | - |
extracellular | the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein | Escherichia coli | - |
- |
periplasm | the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein | Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the signal peptide is removed from the ppPGA during translocation into the periplasm. In the periplasm pPGA undergoes posttranslational modification that results in active PGA. Processing of the PGA is temperature-dependent | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-phenylacetamide benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
penicillin G acylase | - |
Escherichia coli |
PGA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |