Application | Comment | Organism |
---|---|---|
synthesis | enantioselective hydrolysis of N-phenylacetyl-DL-tert-leucine to yield L-tert-leucine. Rate of enzyme-catalyzed reaction is significantly affected by the presence of 2% organic cosolvent. Initial rate falls with increasing logP of the cosolvent, but for logP values less than -0.24, the rate is faster than that in aqueous medium. Yield of L-tert-leucine is above 95% with the enantiomeric excess of more than 99% | Kluyvera cryocrescens |
Organic Solvent | Comment | Organism |
---|---|---|
1,2-ethanediol | 2% v/v, 154% of reaction rate in aqueous environment | Kluyvera cryocrescens |
dimethyl sulfoxide | 2% v/v, 138% of reaction rate in aqueous environment | Kluyvera cryocrescens |
dioxane | 2% v/v, 117% of reaction rate in aqueous environment | Kluyvera cryocrescens |
Glycerol | 2% v/v, 179% of reaction rate in aqueous environment | Kluyvera cryocrescens |
Methanol | 2% v/v, 126% of reaction rate in aqueous environment | Kluyvera cryocrescens |
N,N-dimethylformamide | 2% v/v, 136% of reaction rate in aqueous environment | Kluyvera cryocrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyvera cryocrescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-phenylacetyl-DL-tert-leucine + H2O | enantioselective hydrolysis | Kluyvera cryocrescens | L-tert-leucine + phenylacetate | - |
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