Cloned (Comment) | Organism |
---|---|
a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from K. citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli | Escherichia coli |
a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from Kluyvera citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli | Kluyvera cryocrescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from K. citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli. In comparison with the two wild-type enzymes the hybrid enzyme has a higher turnover-number for benzylpenicillin, ampicillin and 6-nitro-3-phenylacetamido-benzoic acid. The KM-values are between the values of the wild-type enzymes or close ro that of K. citrophila | Escherichia coli |
additional information | a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from Kluyvera citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli. In comparison with the two wild-type enzymes the hybrid enzyme has a higher turnover-number for benzylpenicillin, ampicillin and 6-nitro-3-phenylacetamido-benzoic acid. The Km-values are between the values of the wild-type enzymes or close ro that of Kluyvera citrophila | Kluyvera cryocrescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
benzylpenicillin | pH 7.5, 25°C | Escherichia coli | |
0.018 | - |
benzylpenicillin | pH 7.5, 25°C | Kluyvera cryocrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
ATCC 11105 | - |
Kluyvera cryocrescens | - |
ATCC 21285 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-phenylacetamido-benzoic acid + H2O | - |
Escherichia coli | ? | - |
? | |
6-nitro-3-phenylacetamido-benzoic acid + H2O | - |
Kluyvera cryocrescens | ? | - |
? | |
ampicillin + H2O | - |
Escherichia coli | ? | - |
? | |
ampicillin + H2O | - |
Kluyvera cryocrescens | ? | - |
? | |
benzylpenicillin + H2O | - |
Escherichia coli | phenylacetic acid + 6-aminopenicillanate | - |
? | |
benzylpenicillin + H2O | - |
Kluyvera cryocrescens | phenylacetic acid + 6-aminopenicillanate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
benzylpenicillin | pH 7.5, 25°C | Escherichia coli | |
56.5 | - |
benzylpenicillin | pH 7.5, 25°C | Kluyvera cryocrescens |