Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
benzylpenicillin | pH 7.5, 25°C, enzyme form PA5..5 and PA5.3 | Alcaligenes faecalis | |
0.01 | - |
benzylpenicillin | pH 7.5, 25°C | Escherichia coli | |
0.012 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C, enzyme form PA5.3 | Alcaligenes faecalis | |
0.017 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C, enzyme form PA5.5 | Alcaligenes faecalis | |
0.02 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22540 | - |
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI | Alcaligenes faecalis |
22920 | - |
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI | Alcaligenes faecalis |
62564 | - |
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI | Alcaligenes faecalis |
62568 | - |
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Alcaligenes faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
3.8 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.3 and 3.2 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.5 | Alcaligenes faecalis |
Storage Stability | Organism |
---|---|
4 °C, half-life is 6 h | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-phenylacetamidobenzoic acid + H2O | - |
Alcaligenes faecalis | ? | - |
? | |
6-nitro-3-phenylacetamidobenzoic acid + H2O | - |
Escherichia coli | ? | - |
? | |
benzylpenicillin + H2O | - |
Alcaligenes faecalis | phenylacetic acid + 6-aminopenicillanate | - |
? | |
benzylpenicillin + H2O | - |
Escherichia coli | phenylacetic acid + 6-aminopenicillanate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI | Alcaligenes faecalis |
? | ? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI | Alcaligenes faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 6 h | Alcaligenes faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C | Escherichia coli | |
50 | - |
benzylpenicillin | pH 7.5, 25°C | Escherichia coli | |
75 | - |
benzylpenicillin | pH 7.5, 25°C, enzyme form PA5..5 | Alcaligenes faecalis | |
77 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C, enzyme form PA5..5 | Alcaligenes faecalis | |
80 | - |
benzylpenicillin | pH 7.5, 25°C, enzyme form PA5..3 | Alcaligenes faecalis | |
82 | - |
6-nitro-3-phenylacetamidobenzoic acid | pH 7.5, 25°C, enzyme form PA5..3 | Alcaligenes faecalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alcaligenes faecalis | enzyme form PA5.3, isoelectric focusing | - |
5.3 |
Alcaligenes faecalis | enzyme form PA5.5, isoelectric focusing | - |
5.5 |
Escherichia coli | isoelectric focusing | - |
7 |