Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.11 extracted from

  • Moenster, A.; Villain, L.; Scheper, T.; Beutel, S.
    One-step-purification of penicillin G amidase from cell lysate using ion-exchange membrane adsorbers (2013), J. Membr. Sci., 444, 359-364.
No PubMed abstract available
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expresssion of the enzyme in Escherichia coli strain 5KpHM12 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin G + H2O Escherichia coli
-
 phenylacetate + 6-aminopenicillanate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain 5KpHM12 culture supernatant by anion and cation exchange chromatographic high-throughput absorption membranes, method development and evaluation, binding dynamics, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
 Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetate
-
 ?
penicillin G + H2O
-
 Escherichia coli phenylacetate + 6-aminopenicillanate
-
 ?

Subunits

Subunits Comment Organism
? x * 70000 Escherichia coli

Synonyms

Synonyms Comment Organism
Penicillin G amidase
-
 Escherichia coli
PGA
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli
-
-
 8.5