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Literature summary for 3.5.1.11 extracted from
- Purification and properties of penicillin amidase from Bacillus megaterium (1967), J. Bacteriol., 93, 302-308.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-aminopenicillanate | product inhibition | Priestia megaterium |  |
| Co2+ | - |
Priestia megaterium | |
| Fe2+ | - |
Priestia megaterium | |
| Mn2+ | - |
Priestia megaterium | |
| additional information | no inhibition with sulfhydryl reagents | Priestia megaterium | |
| Ni2+ | - |
Priestia megaterium | |
| phenylacetic acid | product inhibition, competitve | Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
benzylpenicillin | 37°C, pH 8.7 | Priestia megaterium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no stimulation with divalent metal ions | Priestia megaterium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
sedimentation equilibrium centrifugation | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylpenicillin + H2O | Priestia megaterium | highly specific | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| benzylpenicillin + H2O | Priestia megaterium 14945 | highly specific | phenylacetic acid + 6-aminopenicillanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
| Priestia megaterium 14945 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.7 | - |
substrate benzylpenicillin, purified enzyme, 37°C, pH 8.7 | Priestia megaterium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.05 M phosphate buffer, pH 7.0, purified enzyme, stable for at least 3 months | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allylmercaptomethylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| benzylpenicillin + H2O | highly specific | Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| benzylpenicillin + H2O | complete hydrolysis at low substrate concentrations | Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| benzylpenicillin + H2O | highly specific | Priestia megaterium 14945 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| benzylpenicillin + H2O | complete hydrolysis at low substrate concentrations | Priestia megaterium 14945 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| beta-(alpha-toluylamido)-propionic acid + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| D,L-N-phenylacetylalanine + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| delta2-pentenylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| ethylthiomethylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| additional information | no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin | Priestia megaterium | ? | - |
? | |
| additional information | no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin | Priestia megaterium 14945 | ? | - |
? | |
| N-methylphenylacetamide + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| penicillin V + H2O | poor substrate | Priestia megaterium | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
| penicillin V + H2O | poor substrate | Priestia megaterium 14945 | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
| phenoxyethylpenicillin + H2O | poor substrate | Priestia megaterium | 3-phenoxypropanoic acid + 6-aminopenicillanic acid | - |
? | |
| phenylacetamide + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
| phenylacetamide + H2O | poor substrate | Priestia megaterium 14945 | ? | - |
? | |
| phenylacetamide + H2O | poor substrate | Priestia megaterium | NH3 + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| penicillin G acylase | - |
Priestia megaterium |
| PGA | - |
Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -20 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
| 5 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
| 24 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
| 37 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
| 45 | - |
pH 8.4, protein concentration of 1 mg/ml, 70% of activity lost within 8 hours | Priestia megaterium |
| 60 | - |
pH 8.4, protein concentration of 1 mg/ml, complete denaturation within 8 hours | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | rapid decrease in activity when the pH is raised above 9.5 or lowered below 7.5 | Priestia megaterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | highest stability when incubated at 24°C for 9 h | Priestia megaterium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
6-aminopenicillanate | 37°C, pH 8.7 | Priestia megaterium | |
| 450 | - |
phenylacetic acid | 37°C, pH 8.7 | Priestia megaterium | |
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