Protein Variants | Comment | Organism |
---|---|---|
V56R | mutation of beta-subunit, 12% of wild-type activity with substrate penicillin G, 230% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R | mutation of beta-subunit, plus F146Y in alpha-subunit, 5% of wild-type activity with substrate penicillin G, 280% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R/T32Y | mutation of beta-subunit, plus F146Y in alpha-subunit, 2.3% of wild-type activity with substrate penicillin G, 460% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R/T32Y/I177Y | mutation of beta-subunit, plus F146Y in alpha-subunit, 1.8% of wild-type activity with substrate penicillin G, 490% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R/T32Y/I177Y/P49Q | mutation of beta-subunit, plus F146Y in alpha-subunit, 1.3% of wild-type activity with substrate penicillin G, 510% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R/T32Y/I177Y/P49Q/W154Y | mutation of beta-subunit, plus F146Y in alpha-subunit, 1.2% of wild-type activity with substrate penicillin G, 600% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
V56R/T32Y/I177Y/P49Q/W154Y/F24L | mutation of beta-subunit, plus F146Y in alpha-subunit, 0.3% of wild-type activity with substrate penicillin G, 760% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
substrate glutaryl-7-aminocephalosporanic acid, wild-type, pH 8.0 | Escherichia coli |
666 | - |
substrate penicillin G, wild-type, pH 8.0 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Escherichia coli | glutarate + 7-aminocephalosporanic acid | - |
? | |
penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? |