Application | Comment | Organism |
---|---|---|
synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and Bacillus subtilis, high expression rate of extracellular enzyme in Bacillus subtilis | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE, native mass by gel filtration | Priestia megaterium |
115000 | - |
gel filtration | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | Priestia megaterium | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
strain ATCC 14945 | - |
Purification (Comment) | Organism |
---|---|
from cell culture medium of transgenic Bacillus subtilis | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
ampicillin as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
0.23 | - |
cephradine as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
0.56 | - |
cephalexin as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
0.67 | - |
cephaloglycin as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
1.24 | - |
cephaloridine as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
1.36 | - |
cefamandole as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
1.58 | - |
cephalothin as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
1.86 | - |
penicillin G as subsrate, purified enzyme, 37°C, pH 6.8 | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-amino-desacetoxycephalosporanic acid + D-alpha-phenylglycine methylester | acylating activity | Priestia megaterium | ? | - |
? | |
7-aminocephalosporanic acid + D-alpha-phenylglycine methylester | acylating activity | Priestia megaterium | ? | - |
? | |
cefamandole + H2O | - |
Priestia megaterium | (6R,7R)-7-amino-3-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-hydroxy(phenyl)acetic acid | - |
? | |
cephalexin + H2O | - |
Priestia megaterium | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephaloglycin + H2O | - |
Priestia megaterium | ? | - |
? | |
cephaloridine + H2O | - |
Priestia megaterium | (6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid | - |
? | |
cephalothin + H2O | - |
Priestia megaterium | (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid | - |
? | |
cephradine + H2O | - |
Priestia megaterium | (2R)-amino(cyclohexa-1,4-dien-1-yl)acetic acid + (6R,7R)-7-amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
additional information | no activity with cephapirin, cefotaximen cephalosporin, and penicillin N | Priestia megaterium | ? | - |
? | |
penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE, native mass by gel filtration | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
penicillin G acylase | - |
Priestia megaterium |
PGA | - |
Priestia megaterium |