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Literature summary for 3.5.1.11 extracted from

  • Rathinaswamy, P.; Gaikwad, S.M.; Suresh, C.G.; Prabhune, A.A.; Brannigan, J.A.; Dodson, G.G.; Pundle, A.V.
    Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis (2012), Int. J. Biol. Macromol., 50, 25-30.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene yxeI, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C1A no activity, catalytic residue Bacillus subtilis
C1S no activity, catalytic residue Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
cephalosporin C 65% inhibition; 65% inhibition at 1 mM Bacillus subtilis
cephalosporin G 79% inhibition; 79% inhibition at 1 mM Bacillus subtilis
deoxycholic acid slight inhibition Bacillus subtilis
glycodeoxycholic acid slight inhibition Bacillus subtilis
additional information poor inhibition by Fe2+, Mn2+, and Co2+ Bacillus subtilis
Ni2+ slight inhibition Bacillus subtilis
phenoxyacetic acid slight inhibition Bacillus subtilis
taurodeoxycholic acid slight inhibition Bacillus subtilis
Zn2+ slight inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis–Menten kinetics Bacillus subtilis
0.63
-
2-nitro-5-(phenoxyacetamido)-benzoic acid pH 6.6, 40°C Bacillus subtilis
40
-
Penicillin V pH 6.6, 40°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37008
-
4 * 37008, MALDI, gel filtration Bacillus subtilis
37008
-
4 * 37008, mass spectrometry Bacillus subtilis
148000
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis Q2HPP6
-
-
Bacillus subtilis Q2HPP6 gene yxeI
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction chromatography Bacillus subtilis
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction chrmatography and ultrafiltration Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
substrate penicillin V, pH 6.6, 40°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
-
Bacillus subtilis ?
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
-
Bacillus subtilis 5-amino-2-nitrobenzoic acid + phenoxyacetate
-
?
additional information substrate specificity, overview. No activity with ampicillin, amoxicillin, or cephalosporin. Penicillin G is a poor substrate Bacillus subtilis ?
-
?
penicillin V + H2O penicillin G: negligible activity, ampicillin or amoxicillin: no activity Bacillus subtilis 6-aminopenicillanic acid + phenoxyacetic acid
-
?
penicillin V + H2O
-
Bacillus subtilis phenoxyacetate + 6-aminopenicillinate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 37008, MALDI, gel filtration Bacillus subtilis
homotetramer 4 * 37008, mass spectrometry Bacillus subtilis

Synonyms

Synonyms Comment Organism
penicillin acylase
-
Bacillus subtilis
penicillin V acylase
-
Bacillus subtilis
YxeI
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
after 4 h at 40°C 80% residual activity, after 4 h at 50°C 40% residual activity, after 4 h at 55°C 20% residual activity, at 60°C rapidly inactivated Bacillus subtilis
50
-
purified recombinant enzyme, pH 6.6, 15 min, completely stable up to Bacillus subtilis
55
-
purified recombinant enzyme, pH 6.6, 15 min, 80% activity remaining Bacillus subtilis
60
-
purified recombinant enzyme, pH 6.6, 15 min, inactivation Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
assay at Bacillus subtilis
6.6 7.4
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range Bacillus subtilis
5.5 9
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
purified recombinant enzyme, 4 h at room temperature, over 50% activity remaning Bacillus subtilis
5 12 10 min at 40°C, more than 50% of the activity is retained Bacillus subtilis
6 8 purified recombinant enzyme, 4 h at room temperature, completely stable at Bacillus subtilis
10
-
purified recombinant enzyme, 4 h at room temperature, over 50% activity remaning Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis isoelectric focusing
-
5.3

General Information

General Information Comment Organism
evolution the enzyme belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily of proteins, which is characterized by a distinct alphabetabetaalpha fold (Ntn fold). Based on their substrate specificity, they are classified into penicillin V acylases (PVA), penicillin G acylases (PGA) and ampicillin acylases, which preferentially cleave penicillin V (PenV), penicillin G (PenG) and ampicillin, respectively Bacillus subtilis