Cloned (Comment) | Organism |
---|---|
gene yxeI, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C1A | no activity, catalytic residue | Bacillus subtilis |
C1S | no activity, catalytic residue | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cephalosporin C | 65% inhibition; 65% inhibition at 1 mM | Bacillus subtilis | |
cephalosporin G | 79% inhibition; 79% inhibition at 1 mM | Bacillus subtilis | |
deoxycholic acid | slight inhibition | Bacillus subtilis | |
glycodeoxycholic acid | slight inhibition | Bacillus subtilis | |
additional information | poor inhibition by Fe2+, Mn2+, and Co2+ | Bacillus subtilis | |
Ni2+ | slight inhibition | Bacillus subtilis | |
phenoxyacetic acid | slight inhibition | Bacillus subtilis | |
taurodeoxycholic acid | slight inhibition | Bacillus subtilis | |
Zn2+ | slight inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MichaelisMenten kinetics | Bacillus subtilis | |
0.63 | - |
2-nitro-5-(phenoxyacetamido)-benzoic acid | pH 6.6, 40°C | Bacillus subtilis | |
40 | - |
Penicillin V | pH 6.6, 40°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37008 | - |
4 * 37008, MALDI, gel filtration | Bacillus subtilis |
37008 | - |
4 * 37008, mass spectrometry | Bacillus subtilis |
148000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q2HPP6 | - |
- |
Bacillus subtilis | Q2HPP6 | gene yxeI | - |
Purification (Comment) | Organism |
---|---|
hydrophobic interaction chromatography | Bacillus subtilis |
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction chrmatography and ultrafiltration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
substrate penicillin V, pH 6.6, 40°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O | - |
Bacillus subtilis | ? | - |
? | |
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O | - |
Bacillus subtilis | 5-amino-2-nitrobenzoic acid + phenoxyacetate | - |
? | |
additional information | substrate specificity, overview. No activity with ampicillin, amoxicillin, or cephalosporin. Penicillin G is a poor substrate | Bacillus subtilis | ? | - |
? | |
penicillin V + H2O | penicillin G: negligible activity, ampicillin or amoxicillin: no activity | Bacillus subtilis | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
penicillin V + H2O | - |
Bacillus subtilis | phenoxyacetate + 6-aminopenicillinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37008, MALDI, gel filtration | Bacillus subtilis |
homotetramer | 4 * 37008, mass spectrometry | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
penicillin acylase | - |
Bacillus subtilis |
penicillin V acylase | - |
Bacillus subtilis |
YxeI | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
after 4 h at 40°C 80% residual activity, after 4 h at 50°C 40% residual activity, after 4 h at 55°C 20% residual activity, at 60°C rapidly inactivated | Bacillus subtilis |
50 | - |
purified recombinant enzyme, pH 6.6, 15 min, completely stable up to | Bacillus subtilis |
55 | - |
purified recombinant enzyme, pH 6.6, 15 min, 80% activity remaining | Bacillus subtilis |
60 | - |
purified recombinant enzyme, pH 6.6, 15 min, inactivation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at | Bacillus subtilis |
6.6 | 7.4 | - |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range | Bacillus subtilis |
5.5 | 9 | - |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
purified recombinant enzyme, 4 h at room temperature, over 50% activity remaning | Bacillus subtilis |
5 | 12 | 10 min at 40°C, more than 50% of the activity is retained | Bacillus subtilis |
6 | 8 | purified recombinant enzyme, 4 h at room temperature, completely stable at | Bacillus subtilis |
10 | - |
purified recombinant enzyme, 4 h at room temperature, over 50% activity remaning | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | isoelectric focusing | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily of proteins, which is characterized by a distinct alphabetabetaalpha fold (Ntn fold). Based on their substrate specificity, they are classified into penicillin V acylases (PVA), penicillin G acylases (PGA) and ampicillin acylases, which preferentially cleave penicillin V (PenV), penicillin G (PenG) and ampicillin, respectively | Bacillus subtilis |