KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0176 | - |
6-nitro-3-phenylacetamide benzoic acid | pH 7.5, 37°C | Kluyvera cryocrescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
62000 | - |
x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyvera cryocrescens | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
His-tagged enzyme expressed in Escherichia coli | Kluyvera cryocrescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.3 | - |
pH 7.5, 37°C | Kluyvera cryocrescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-phenylacetamide benzoic acid + H2O | - |
Kluyvera cryocrescens | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Kluyvera cryocrescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable for at least 10 days | Kluyvera cryocrescens |
22 | - |
10 days, 76% residual activity | Kluyvera cryocrescens |
40 | - |
degradation above | Kluyvera cryocrescens |
60 | - |
30 min, complete loss of activity | Kluyvera cryocrescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Kluyvera cryocrescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
more than 40% of maximum activity | Kluyvera cryocrescens |
5 | 10 | plateau of activity within this range | Kluyvera cryocrescens |