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Literature summary for 3.5.1.11 extracted from
- Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945 (1995), FEMS Microbiol. Lett., 125, 287-292.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli HB101, enzyme is expressed as cytoplasmic enzyme, enzyme production not inducible by phenylacetic acid, expressed in Escherichia coli TG1, enzyme production inducible by addition of IPTG, intracellular expression | Priestia megaterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin + H2O | Priestia megaterium | - |
a carboxylate + 6-aminopenicillanate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | Q60136 | penicillin G acylase precursor; strain ATCC 14945 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin + H2O | - |
Priestia megaterium | a carboxylate + 6-aminopenicillanate | - |
? | |
| penicillin G + H2O | - |
Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| penicillin G acylase | - |
Priestia megaterium |
| PGA | - |
Priestia megaterium |
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