Protein Variants | Comment | Organism |
---|---|---|
additional information | construction hybrids of the penicillin acylase-encoding genes from Eacherichia coli and Kluyvera cryocrescens, with additional point mutations. The hybrid enzymes display a 40-90% increase in the relative rate of acyl transfer to the beta-lactam nucleus during ampicillin synthesis. This increase is not accompanied by a reduction of synthetic activity. Similar improvements in acyl transfer are obtained for the synthesis of amoxicillin, cephalexin and cefadroxil making the new hybrid enzymes interesting candidates for the biocatalytic synthesis of several beta-lactam antibiotics | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | P06875 | - |
- |