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Literature summary for 3.5.1.11 extracted from

  • Philem, P.D.; Sonalkar, V.V.; Dharne, M.S.; Prabhune, A.A.
    Purification and partial characterization of novel penicillin V acylase from Acinetobacter sp. AP24 isolated from Loktak Lake, an Indo-Burma biodiversity hotspot (2016), Prep. Biochem. Biotechnol., 46, 524-530 .
    View publication on PubMed
Search for more literature for 3.5.1.11

Application

Application Comment Organism
industry penicillin acylase are industrially important enzymes that break down different penicillins to give 6-amino penicillanic acid, the precursor for synthesis of semi synthetic penicillins and cephalosporins antibiotics Acinetobacter sp. AP-24
pharmacology penicillin acylases are pharmaceutically important enzymes widely used in the synthesis of semi synthetic beta lactam antibiotics Acinetobacter sp. AP-24

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acinetobacter sp. AP-24

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Acinetobacter sp. AP-24 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137000
-
 gel filtration Acinetobacter sp. AP-24

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. AP-24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter sp. AP-24

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O 2.4% of the activity compared to penicillin V Acinetobacter sp. AP-24 7-aminocephalosporanic acid + (R)-2-aminohexadioic acid
-
 ?
additional information no activity with cefaclor, cephalexin, ampicillin, dicloxacillin or cephalosporin G Acinetobacter sp. AP-24 ?
-
 ?
penicillin V + H2O the enzyme is highly specific towards penicillin V Acinetobacter sp. AP-24 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 34000, MALDI-TOF Acinetobacter sp. AP-24

Synonyms

Synonyms Comment Organism
penicillin V acylase
-
 Acinetobacter sp. AP-24

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Acinetobacter sp. AP-24

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 60% of maximal activity, 45°C: about 45% of maximal activity Acinetobacter sp. AP-24

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 h, about 20% loss of activity Acinetobacter sp. AP-24
40
-
 1 h, about 25% loss of activity Acinetobacter sp. AP-24
50
-
 1 h, about 90% loss of activity Acinetobacter sp. AP-24

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Acinetobacter sp. AP-24

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity Acinetobacter sp. AP-24

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 25°C, 2 h, stable Acinetobacter sp. AP-24

General Information

General Information Comment Organism
metabolism the enzyme is producde in a constitutive manner Acinetobacter sp. AP-24