Reference on EC 3.4.21.62 - Subtilisin
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ottesen, M.; Svendsen, I.
The subtilisins
Methods Enzymol.
19
199-215
1970
Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus sp. (in: Bacteria)
-
Kraut, J.
Subtilisin: X-ray structure
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
3
547-560
1971
Bacillus amyloliquefaciens, Bacillus sp. (in: Bacteria)
-
Markland, F.S.; Smith, E.L.
Subtilisins: primary structure, chemical and physical properties
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
3
561-608
1971
Bacillus amyloliquefaciens, Bacillus sp. (in: Bacteria)
-
Nedkov, P.; Oberthur, W.; Braunitzer, G.
Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN, Carlsberg and amylosacchariticus
Biol. Chem. Hoppe-Seyler
366
421-430
1985
Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DY
Markland, F.S.; Brown, D.M.; Smith, E.L.
Subtilisin Amylosacchariticus. I. Physicochemical characterization
J. Biol. Chem.
247
5596-5601
1972
Bacillus sp. (in: Bacteria)
Wells, J.A.; Estell, D.A.
Subtilisin - an enzyme designed to be engineered
Trends Biochem. Sci.
13
291-297
1988
Bacillus amyloliquefaciens
Kamal, M.; Höög, J.O.; Kaiser, R.; Shafqat, J.; Razzaki, T.; Zaidi, Z.H.; Jörnvall, H.
Isolation, characterization and structure of subtilisin from a thermostable Bacillus subtilis isolate
FEBS Lett.
374
363-366
1995
Bacillus subtilis, Bacillus subtilis RT-5
Katayama, H.; Soezima, Y.; Fujimura, S.; Terada, S.; Kimoto, E.
Property and amino acid sequence of a subtilisin inhibitor from seeds of beach canavalia (Canavalia lineata)
Biosci. Biotechnol. Biochem.
58
2004-2008
1994
Bacillus sp. (in: Bacteria)
Terabe, M.; Kojima, S.; Taguchi, S.; Momose, H.; Miura, K.
Primary structure and inhibitory properties of a subtilisin-chymotrypsin inhibitor from Streptomyces virginiae
Eur. J. Biochem.
226
627-632
1994
Bacillus sp. (in: Bacteria)
Occhiato, E.; Jones, J.B.
Probing enzyme stereospecificity. Inhibition of alpha-chymotrypsin and subtilisin Carlsberg by chiral amine and amino alcohol derivative
Tetrahedron
52
4199-4214
1996
Bacillus sp. (in: Bacteria)
-
Lange, G.; Betzel, C.; Branner, S.; Wilson, K.S.
Crystallographic studies of Savinase, a subtilisin-like proteinase, at pH 10.5
Eur. J. Biochem.
224
507-518
1994
Bacillus sp. (in: Bacteria)
Matsubara, M.; Kurimoto, E.; Kojima, S.; Miura, K.; Sakai, T.
Achievement of renaturation of subtilisin BPN by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor
FEBS Lett.
342
193-196
1994
Bacillus sp. (in: Bacteria)
Heinz, D.W.; Priestle, J.P.; Rahuel, J.; Wilson, K.S.; Grutter, M.G.
Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes
J. Mol. Biol.
217
353-371
1991
Bacillus sp. (in: Bacteria)
Takeuchi, Y.; Satow, Y.; Nakamura, K.T.; Mitsui, Y.
Refined crystal structure of the complex of subtilisin BPN and Streptomyces subtilisin inhibitor at 1.8 A resolution
J. Mol. Biol.
221
309-325
1991
Bacillus sp. (in: Bacteria)
Stahl, M.L.; Ferrari, E.
Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation
J. Bacteriol.
158
411-418
1984
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis 1168, Bacillus sp. (in: Bacteria) 1168
Bodhe, A.M.
Purification and properties of a subtilisin inhibitor and an associated trypsin inhibitor from Dolichos biflorus
Biochim. Biophys. Acta
1073
11-17
1991
Bacillus amyloliquefaciens
Wells, J.A.; Powers, D.B.
In vivo formation and stability of engineered disulfide bonds in subtilisin
J. Biol. Chem.
261
6564-6570
1986
Bacillus amyloliquefaciens
Steinmetz, A.C.U.; Demuth, H.U.; Ringe, D.
Inactivation of subtilisin Carlsberg by N-((tert-butoxycarbonyl)alanylprolylphenylalanyl)-O-benzoylhydroxyl- amine: formation of a covalent enzyme-inhibitor linkage in the form of a carbamate derivative
Biochemistry
33
10535-10544
1994
Bacillus sp. (in: Bacteria)
Sobek, H.; Hecht, H.J.; Hofmann, B.; Aehle, W.; Schomburg, D.
Crystal structure of an alkaline protease from Bacillus alcalophilus at 2.4 A resolution
FEBS Lett.
274
57-60
1990
Alkalihalobacillus alcalophilus, Alkalihalobacillus alcalophilus Opticlean
Hejgaard, J.; Dam, J.; Peterson, L.C.; Bjorn, S.E.
Primary structure and specificity of the major serine proteinase inhibitor of amaranth (Amaranthus caudatus L.) seeds
Biochim. Biophys. Acta
1204
68-74
1994
Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus sp. (in: Bacteria)
Terada, S.; Fujimura, S.; Katayama, H.; Nagasawa, M.; Kimoto, E.
Purification and characterization of two Kunitz family subtilisin inhibitors from seeds of Canavalia lineata
J. Biochem.
115
392-396
1994
Bacillus sp. (in: Bacteria)
Eder, J.; Rheinnecker, M.; Fersht, A.R.
Folding of subtilisin BPN: characterization of a folding intermediate
Biochemistry
32
18-26
1993
Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DB104
Betzel, C.; Visanji, M.; Eschenburg, S.; Wilson, K.S.; Peters, K.; Fittkau, S.; Singh, T.P.; Genov, N.
Crystallization and preliminary X-ray analysis of subtilisin DY, a natural mutant of subtilisin Carlsberg
Arch. Biochem. Biophys.
302
499-502
1993
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DY
Goddette, D.W.; Paech, C.; Yang, S.S.; Mielenz, J.R.; Bystroff, C.; Wilke, M.E.; Fletterick, R.J.
The crystal structure of the Bacillus lentus alkaline protease, subtilisin BL, at 1.4 A resolution
J. Mol. Biol.
228
580-595
1992
Lederbergia lentus
Economou, C.; Chen, K.; Arnold, F.H.
Random mutagenesis to enhance the activity of subtilisin in organic solvents: characterization of Q103R subtilisin E
Biotechnol. Bioeng.
39
658-662
1992
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DB428
Bryan, P.N.; Rollence, M.L.; Pantoliano, M.W.; Wood, J.; Finzel, B.C.; Gilliland, G.L.; Howard, A.J.; Poulos, T.L.
Proteases of enhanced stability: characterization of a thermostable variant of subtilisin
Proteins Struct. Funct. Genet.
1
326-334
1986
Bacillus amyloliquefaciens
Neidhart, D.J.; Petsko, G.A.
The refined crystal structure of subtilisin Carlsberg at 2.5 A resolution
Protein Eng.
2
271-276
1988
Bacillus sp. (in: Bacteria)
Peters, K.; Pauli, D.; Hache, H.; Boteva, R.N.; Genov, N.C.; Fittkau, S.
Subtilisin DY kinetic characterization and comparison with related proteinases
Curr. Microbiol.
18
171-177
1989
Bacillus sp. (in: Bacteria)
-
Ohta, Y.; Inouye, M.
Pro-subtilisin E: purification and characterization of its autoprocessing to active subtilisin E in vitro
Mol. Microbiol.
4
295-304
1990
Bacillus subtilis, Bacillus sp. (in: Bacteria)
Russell, A.J.; Fersht, A.R.
Commercial samples of subtilisin BPN'
Nature
321
733
1986
Bacillus sp. (in: Bacteria)
-
Thomas, P.G.; Russell, A.J.; Fersht, A.R.
Tailoring the pH dependence of enzyme catalysis using protein engineering
Nature
318
375-376
1985
Bacillus amyloliquefaciens, Bacillus sp. (in: Bacteria)
-
Kourteva, Y.; Boteva, R.
A novel extracellular subtilisin inhibitor produced by a Streptomyces sp
FEBS Lett.
247
468-470
1989
Bacillus sp. (in: Bacteria)
Betzel, C.; Klupsch, S.; Papendorf, G.; Hastrup, S.; Branner, S.; Wilson, K.S.
Crystal structure of the alkaline proteinase Savinase from Bacillus lentus at 1.4 A resolution
J. Mol. Biol.
223
427-445
1992
Lederbergia lentus
Gololobov,M.Y.; Morozova, I.P.; Vojushina, T.L.; Timokhina, E.A.; Stepanov, V.M.
Subtilisin from Bacillus subtilis strain 72. The influence of substrate structure, temperature and pH on catalytic properties
Biochim. Biophys. Acta
1118
267-276
1992
Bacillus subtilis, Bacillus subtilis 72
Yamagata, Y.; Isshiki, K.; Ichishima, E.
Subtilisin Sendai from alkalophilic Bacillus sp.: molecular and enzymatic properties of the enzyme and molecular cloning and characterization of the gene, aprS
Enzyme Microb. Technol.
17
653-663
1995
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) G-825-6
Chu, N.M.; Chao, Y.; Bi, R.C.
The 2 A crystal structure of subtilisin E with PMSF inhibitor
Protein Eng.
8
211-215
1995
Bacillus subtilis, Bacillus sp. (in: Bacteria)
Mitsui, Y.; Satow, Y.; Watanabe, Y.; Hirono, S.; Iitaka, Y.
Crystal structures of Streptomyces subtilisin inhibitor and its complex with subtilisin BPN
Nature
277
447-452
1979
Bacillus sp. (in: Bacteria)
Bode, W.; Papamokos, E.; Musil, D.; Seemueller, U.; Fritz, H.
Refined 1.2 A crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin c. Molecular structure of eglin and its detailed interaction with subtilisin
EMBO J.
5
813-818
1986
Bacillus sp. (in: Bacteria)
Bode, W.; Papamokos, E.; Musil, D.
The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry
Eur. J. Biochem.
166
673-692
1987
Bacillus sp. (in: Bacteria)
Gilliland, G.L.; Howard, A.J.; Winborne, E.L.; Poulos, T.L.; Stewart, D.B.; Durham, D.R.
Crystallization and preliminary x-ray diffraction studies of subtilisin GX from Bacillus sp. GX6644
J. Biol. Chem.
262
4280-4283
1987
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GX6644
Genov, N.; Shopova, M.; Boteva, R.; Jori, G.; Ricchelli, F.
Chemical, photochemical and spectroscopic characterization of an alkaline proteinase from Bacillus subtilis variant DY
Biochem. J.
207
193-200
1982
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DY
Ricchelli, F.; Jori, G.; Filippi, B.; Boteva, R.; Shopova, M.; Genov, N.
Effects of pH and urea on the conformational properties of subtilisin DY
Biochem. J.
207
201-205
1982
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus subtilis DY
Chandrasekaran, S.; Dhar, S.C.
Purification of subtilisin by single-step affinity chromatography
Anal. Biochem.
150
141-144
1985
Bacillus sp. (in: Bacteria)
Vartak, H.G.; Bodhe, A.M.; Paranjpe, S.V.; Jagannathan, V.
Proteinase inhibitors from Vigna unguiculata subsp. cylindrica. II. Inhibitors of subtilisin and trypsin
Arch. Biochem. Biophys.
204
129-133
1980
Bacillus subtilis
Vartak, H.G.; Rele, M.V.; Jagannathan, V.
Proteinase inhibitors from Vigna unguiculata subsp. cylindrica. III. Properties and kinetics of inhibitors of papain, subtilisin, and trypsin
Arch. Biochem. Biophys.
204
134-140
1980
Bacillus subtilis
Lindquist, R.N.; Terry, C.
Inhibition of subtilisin by boronic acids, potential analogs of tetrahedral reaction intermediates
Arch. Biochem. Biophys.
160
135-144
1974
Bacillus sp. (in: Bacteria)
Svensson, B.
Preparation and enzymatic properties of subtilisin Novo chemically attached to soluble DEAE-dextran and insoluble DEAE-sephadex
Biochim. Biophys. Acta
429
954-963
1976
Bacillus sp. (in: Bacteria)
Jacobs, M.; Eliasson, M.; Uhlen, M.; Flock, J.I.
Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis
Nucleic Acids Res.
13
8913-8926
1985
Bacillus licheniformis, Bacillus sp. (in: Bacteria)
Wells, J.A.; Ferrari, E.; Henner, D.J.; Estell, D.A.; Chen, E.Y.
Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis
Nucleic Acids Res.
11
7911-7925
1983
Bacillus amyloliquefaciens
Wright, C.S.; Alden, R.A.; Kraut, J.
Crystal structure of a subtilisin BPN complex with N-benzoyl-L-arginine
J. Mol. Biol.
66
283-289
1972
Bacillus sp. (in: Bacteria)
Durham, D.R.
The elastolytic properties of subtilisin GX from alkalophilic Bacillus sp. strain 6644 provides a means of differentiation from other subtilisins
Biochem. Biophys. Res. Commun.
194
1365-1370
1993
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GX6644
Tashiro, M.; Asao, T.; Nakano, H.; Takahashi, K.; Kanamori, M.
Purification and characterization of a subtilisin inhibitor from seeds of foxtail millet, Setaria italica
Agric. Biol. Chem.
55
265-267
1991
Bacillus sp. (in: Bacteria)
Schubert Wright, C.; Alden, R.A.; Kraut, J.
Structure of subtilisin BPN at 2.5 angstrom resolution
Nature
221
235-242
1969
Bacillus sp. (in: Bacteria)
Jang, J.S.; Kang, D.O.; Park, K.S.; Byun, S.M.
Purification and characterization of recombinant Bacillus stearothermophilus subtilisin J
Korean Biochem. J.
26
595-601
1993
Geobacillus stearothermophilus
-
Davail, S.; Feller, G.; Narinx, E.; Gerday, C.
Cold adaptation of proteins. Purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41
J. Biol. Chem.
269
17448-17453
1994
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TA41
Trzmiel, T.; Fortak, M.; Galas, E.
The properties of subtilisin, novo type, immobilized on porous glass
Acta Biotechnol.
15
123-129
1995
Bacillus sp. (in: Bacteria)
-
Dahlen, J.R; Foster, D.C; Kisiel, W.
Human proteinase inhibitor 9 (PI9) is a potent inhibitor of subtilisin A
Biochem. Biophys. Res. Commun.
238
329-333
1997
Bacillus subtilis
Toogood, H.S.; Smith, C.A.; Baker, E.N.; Daniel, R.M.
Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft
Biochem. J.
350
321-328
2000
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Ak1
Koszelak, S.; Ng, J.D.; Day, J.; Ko, T.P.; Greenwood, A.; McPherson, A.
The crystallographic structure of the subtilisin protease from Penicillium cyclopium
Biochemistry
36
6597-6604
1997
Penicillium cyclopium
Kuhn, P.; Knapp, Mark; Soltis, S.M.; Ganshaw, G.; Thoene, M.; Bott, R.
The 0.78 A structure of a serine protease: Bacillus lentus subtilisin
Biochemistry
37
13446-13452
1998
Lederbergia lentus (P29600), Lederbergia lentus
DeSantis, G.; Berglund, P.; Stabile, M.R.; Gold, M.; Jones, J.B.
Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 an S1'pockets of subtilisin Bacillus lentus
Biochemistry
37
5968-5973
1998
Lederbergia lentus
DeSantis, G.; Shang, X.; Jones, J.B.
Toward tailoring of the S1 pocket of subtilisin B. lentus: Chemical modification of mutant enzymes as a strategy for removing specifiity limitations
Biochemistry
38
13391-13397
1999
Lederbergia lentus
Alexander, P.A.; Ruan, B.; Strausberg, S.L.; Bryan, P.N.
Stabilizing mutations and calcium dependent stability of subtilisin
Biochemistry
40
10640-10644
2001
Bacillus amyloliquefaciens
Mitsudo, K.; Jayakumar, A.; Henderson, Y.; Frederick, M.J.; Kang, Y.; Wang, M.; El-Naggar, A.K.; Clayman, G.L.
Inhibition of serine proteinases plasmin, trypsin, subtilisin A, cathepsin G, and elastase by LEKTI: a kinetic analysis
Biochemistry
42
3874-3881
2003
Bacillus licheniformis
Bryan, P.N.
Protein engineering of subtilisin
Biochim. Biophys. Acta
1543
203-222
2000
Bacillus amyloliquefaciens, Bacillus sp. (in: Bacteria)
Davis, B.G.; Khumtaveeporn, K.; Bott, R.R.; Jones, J.B.
Altering the specificity of subtilisin Bacillus lentus through the introduction of positive charge at single amino acid sites
Bioorg. Med. Chem.
7
2303-2311
1999
Lederbergia lentus (P29600), Lederbergia lentus
Kim, S.H.; Choi, N.C.
Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang
Biosci. Biotechnol. Biochem.
64
1722-1725
2000
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) DJ-4
Webb, J.N.; Carpenter, J.F.; Randolph, T.W.
Stability of subtilisin and lysozyme under high hydrostatic pressure
Biotechnol. Prog.
16
630-636
2000
Lederbergia lentus
Eschenburg, S.; Genov, N.; Peters, K.; Fittkau, S.; Stoeva, S.; Wilson, K.S.; Betzel, C.
Crystal structure of subtilisin DY, random mutant of subtilisin Carlsberg
Eur. J. Biochem.
257
309-318
1998
Bacillus licheniformis
Jan, J.; Valle, F.; Bolivar, F.; Merino, E.
Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis
FEMS Microbiol. Lett.
183
9-14
2000
Bacillus subtilis
Smith, C.A.; Toogood, H.S.; Baker, H.M.; Daniel, R.M.; Baker, E.N.
Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 A resolution
J. Mol. Biol.
294
1027-1040
1999
Bacillus sp. (in: Bacteria) (Q45670), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Ak1 (Q45670)
Baeten, A.; Maes, D.; Geerlings, P.
Quantumchemical study of the catalytic triad in subtilisin: the influence of amino acid substitutions on enzymatic activity
J. Theor. Biol.
195
27-40
1998
Bacillus sp. (in: Bacteria)
Takeno, H.; Yamamoto, S.; Tanaka, T.; Sakano, Y.; Kikuchi, Y.
Selection of an RNA molecule that specifically inhibits the protease activity of subtilisin
J. Biochem.
125
1115-1119
1999
Bacillus sp. (in: Bacteria)
Dogru, M.; Baysal, Z.; Aytekin, C.
Suitability of animals' purified milk caseins and their subunit kappa-caseins as substrates for subtilisin and trypsin
Prep. Biochem. Biotechnol.
31
147-154
2001
Bacillus sp. (in: Bacteria)
Schmitke, J.L.; Stern, L.J.; Klibanov, A.M.
Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water
Proc. Natl. Acad. Sci. USA
95
12918-12923
1998
Bacillus licheniformis
Zacharis, E.; Halling, P.J.; Rees, D.G.
Volatile buffers can override the "pH memory" of subtilisin catalysis in organic media
Proc. Natl. Acad. Sci. USA
96
1201-1205
1999
Bacillus licheniformis
Doswald, S.; Estermann, H.; Kupfer, E.; Stadler, H.; Walther, W.; Weisbrod, T.; Wirz, B.; Wostl, W.
Large scale preparation of chiral building blocks for the P3 site of renin inhibitors
Bioorg. Med. Chem.
2
403-410
1994
Bacillus licheniformis, Bacillus sp. (in: Bacteria)
Ricks, E.E.; Estrada-Valdes, M.C.; McLean, T.L.; Iacobucci, G.A.
Highly enantioselective hydrolysis of (R,S)-phenylalanine isopropyl ester by subtilisin Carlsberg. Continuous synthesis of (S)-phenylalanine in a hollow fibre/liquid membrane reactor
Biotechnol. Prog.
8
197-203
1992
Bacillus licheniformis
-
Radisky, E.S.; Kwan, G.; Karen Lu, C.J.; Koshland, D.E.
Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN/chymotrypsin inhibitor 2 complex
Biochemistry
43
13648-13656
2004
Bacillus amyloliquefaciens
Subbian, E.; Yabuta, Y.; Shinde, U.
Positive selection dictates the choice between kinetic and thermodynamic protein folding and stability in subtilases
Biochemistry
43
14348-14360
2004
Bacillus subtilis
Ruan, B.; Fisher, K.E.; Alexander, P.A.; Doroshko, V.; Bryan, P.N.
Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification
Biochemistry
43
14539-14546
2004
Bacillus amyloliquefaciens
Strausberg, S.L.; Ruan, B.; Fisher, K.E.; Alexander, P.A.; Bryan, P.N.
Directed coevolution of stability and catalytic activity in calcium-free subtilisin
Biochemistry
44
3272-3279
2005
Bacillus amyloliquefaciens
Balaban, N.P.; Mardanova, A.M.; Sharipova, M.R.; Gabdrakhmanova, L.A.; Sokolova, E.A.; Rudenskaya, G.N.; Leshchinskaya, I.B.
Purification and characterization of serine proteinase 2 from Bacillus intermedius 3-19
Biochemistry (Moscow)
69
420-426
2004
Bacillus intermedius, Bacillus intermedius Mrz 19
Watanabe, K.; Yoshida, T.; Ueji, S.
The role of conformational flexibility of enzymes in the discrimination between amino acid and ester substrates for the subtilisin-catalyzed reaction in organic solvents
Bioorg. Chem.
32
504-515
2004
Bacillus licheniformis
Yoshimoto, M.; Wang, S.; Fukunaga, K.; Treyer, M.; Walde, P.; Kuboi, R.; Nakao, K.
Enhancement of apparent substrate selectivity of proteinase K encapsulated in liposomes through a cholate-induced alteration of the bilayer permeability
Biotechnol. Bioeng.
85
222-233
2004
Parengyodontium album
Liu, Z.M.; Becker, T.; Neufeld, R.J.
Spherical alginate granules formulated for quick-release active subtilisin
Biotechnol. Prog.
21
568-574
2005
Bacillus sp. (in: Bacteria)
Tian, M.; Kamoun, S.
A two disulfide bridge Kazal domain from Phytophthora exhibits stable inhibitory activity against serine proteases of the subtilisin family
BMC Biochem.
6
15
2005
Solanum lycopersicum
Nakashima, K.; Maruyama, T.; Kamiya, N.; Goto, M.
Comb-shaped poly(ethylene glycol)-modified subtilisin Carlsberg is soluble and highly active in ionic liquids
Chem. Commun. (Camb.)
2005
4297-4299
2005
Bacillus licheniformis
Glowacka, A.E.; Podstawka, E.; Szczesna-Antczak, M.H.; Kalinowska, H.; Antczak, T.
Kinetic and molecular properties of Bacillus subtilis IBTC-3 subtilisin
Comp. Biochem. Physiol. B
140
321-331
2005
Bacillus subtilis, Bacillus subtilis IBTC-3
Choi, N.S.; Chang, K.T.; Jae Maeng, P.; Kim, S.H.
Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4
FEMS Microbiol. Lett.
236
325-331
2004
Bacillus sp. (in: Bacteria) (Q6IT79), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) DJ-4 (Q6IT79)
Hirata, J.; Chung, L.P.; Ariese, F.; Irth, H.; Gooijer, C.
Coupling of size-exclusion chromatography to a continuous assay for subtilisin using a fluorescence resonance energy transfer peptide substrate: testing of two standard inhibitors
J. Chromatogr. A
1081
140-144
2005
Bacillus licheniformis
Kazan, D.; Denizci, A.A.; Oner, M.N.; Erarslan, A.
Purification and characterization of a serine alkaline protease from Bacillus clausii GMBAE 42
J. Ind. Microbiol. Biotechnol.
32
335-344
2005
Alkalihalobacillus clausii, Alkalihalobacillus clausii GMBAE 42
Choi, N.; Ju, S.; Lee, T.Y.; Yoon, K.; Chang, K.; Maeng, P.J.; Kim, S.
Miniscale identification and characterization of subtilisins from Bacillus sp. strains
J. Microbiol. Biotechnol.
15
537-543
2005
Bacillus subtilis, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) DJ-1
-
Wuerges, K.; Pfromm, P.H.; Rezac, M.E.; Czermak, P.
Activation of subtilisin Carlsberg in hexane by lyophilization in the presence of fumed silica
J. Mol. Catal. B
34
18-24
2005
Bacillus licheniformis
-
Chaparro-Riggers, J.F.; Breves, R.; Michels, A.; Maurer, K.; Bornscheuer, U.
A GFP-based assay for the determination of hydrolytic activity and substrate specificity of subtilisins under washing conditions
J. Mol. Catal. B
35
74-77
2005
Lederbergia lentus
-
Hou, R.Z.; Yang, Y.; Huang, Y.B.; Wang, H.; Zhang, N.; Liu, Y.J.; Zhang, X.Z.; Li, G.
Alcalase-catalyzed, kinetically controlled synthesis of a precursor dipeptide of RGDS in organic solvents
Prep. Biochem. Biotechnol.
36
93-105
2006
Bacillus subtilis
Tindbaek, N.; Svendsen, A.; Oestergaard, P.R.; Draborg, H.
Engineering a substrate-specific cold-adapted subtilisin
Protein Eng. Des. Sel.
17
149-156
2004
Lederbergia lentus
Davies, D.A.; Kalinina, N.A.; Samokhvalova, L.V.; Malakhova, G.V.; Scott, G.; Venning, G.; Volynskaya, A.M.; Nesmeyanov, V.A.
Isolation and characterization of the ALP1 protease from Aspergillus fumigatus and its protein inhibitor from Physarum polycephalum
Russ. J. Bioorg. Chem.
31
229-237
2005
Aspergillus fumigatus
-
Uchida, H.; Kondo, D.; Yamashita, S.; Tanaka, T.; Tran, L.H.; Nagano, H.; Uwajima, T.
Purification and properties of a protease produced by Bacillus subtilis CN2 isolated from a Vietnamese fish sauce
World J. Microbiol. Biotechnol.
20
579-582
2004
Bacillus subtilis, Bacillus subtilis CN2
-
Almog, O.; Kogan, A.; de Leeuw, M.; Gdalevsky, G.Y.; Cohen-Luria, R.; Parola, A.H.
Structural insights into cold inactivation of tryptophanase and cold adaptation degrees of subtilisin S41: Minireview
Biopolymers
89
354-359
2008
Bacillus subtilis, Bacillus licheniformis, Lysinibacillus sphaericus, Bacillus amyloliquefaciens (P00782), Vibrio sp. (Q8GB52), Bacillus subtilis TA41
Tanaka, S.; Saito, K.; Chon, H.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon
Acta Crystallogr. Sect. F
62
902-905
2006
Thermococcus kodakarensis
Bye, N.J.; Charnley, A.K.
Regulation of cuticle-degrading subtilisin proteases from the entomopathogenic fungi, Lecanicillium spp: implications for host specificity
Arch. Microbiol.
189
81-92
2008
Lecanicillium longisporum, Akanthomyces muscarius
Fisher, K.E.; Ruan, B.; Alexander, P.A.; Wang, L.; Bryan, P.N.
Mechanism of the kinetically-controlled folding reaction of subtilisin
Biochemistry
46
640-651
2007
Bacillus amyloliquefaciens
Shah, S.; Gupta, M.N.
Obtaining high transesterification activity for subtilisin in ionic liquids
Biochim. Biophys. Acta
1770
94-98
2007
Bacillus licheniformis
Taniguchi, M.; Atiwetin, P.; Hirai, T.; Itoh, M.; Harada, S.; Hara, S.; Kamei, K.
Interaction of subtilisin BPN and recombinant fungal protease inhibitor F from silkworm with substituted P1 site residues
Biosci. Biotechnol. Biochem.
70
1262-1264
2006
Bacillus amyloliquefaciens
Castillo, B.; Sola, R.J.; Ferrer, A.; Barletta, G.; Griebenow, K.
Effect of PEG modification on subtilisin Carlsberg activity, enantioselectivity, and structural dynamics in 1,4-dioxane
Biotechnol. Bioeng.
99
9-17
2008
Bacillus licheniformis
Castillo, B.; Bansal, V.; Ganesan, A.; Halling, P.; Secundo, F.; Ferrer, A.; Griebenow, K.; Barletta, G.
On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg
BMC Biotechnol.
6
51
2006
Bacillus licheniformis
Srimathi, S.; Jayaraman, G.; Narayanan, P.R.
Improved thermodynamic stability of subtilisin Carlsberg by covalent modification
Enzyme Microb. Technol.
39
301-307
2006
Bacillus licheniformis
-
Tanaka, S.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation
J. Mol. Biol.
372
1055-1069
2007
Thermococcus kodakarensis
Pulido, M.A.; Tanaka, S.; Sringiew, C.; You, D.J.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses
J. Mol. Biol.
374
1359-1373
2007
Thermococcus kodakarensis
Fasoli, E.; Castillo, B.; Santos, A.; Silva, E.; Ferrer, A.; Rosario, E.; Griebenow, K.; Secundo, F.; Barletta, G.L.
Activation of subtilisin Carlsberg in organic solvents by methyl-beta -cyclodextrin: Lyoprotection versus substrate and product-complex effect
J. Mol. Catal. B
42
20-26
2006
Bacillus licheniformis
-
Shaw, A.K.; Pal, S.K.
Activity of Subtilisin Carlsberg in macromolecular crowding
J. Photochem. Photobiol. B, Biol.
86
199-206
2007
Bacillus licheniformis
Spreer, A.; Ruechel, R.; Reichard, U.
Characterization of an extracellular subtilisin protease of Rhizopus microsporus and evidence for its expression during invasive rhinoorbital mycosis
Med. Mycol.
44
723-731
2006
Rhizopus microsporus var. rhizopodiformis
Lanigan-Gerdes, S.; Dooley, A.N.; Faull, K.F.; Lazazzera, B.A.
Identification of subtilisin, Epr and Vpr as enzymes that produce CSF, an extracellular signalling peptide of Bacillus subtilis
Mol. Microbiol.
65
1321-1333
2007
Bacillus subtilis, Bacillus licheniformis
Nakashima, K.; Maruyama, T.; Kamiya, N.; Goto, M.
Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin
Org. Biomol. Chem.
4
3462-3467
2006
Bacillus licheniformis
Pulido, M.A.; Koga, Y.; Takano, K.; Kanaya, S.
Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation
Protein Eng. Des. Sel.
20
143-153
2007
Thermococcus kodakarensis
Xie, B.B.; Chen, X.L.; Zhang, X.Y.; He, H.L.; Zhang, Y.Z.; Zhou, B.C.
Predicting protein interaction interfaces from protein sequences: Case studies of subtilisin and phycocyanin
Proteins
71
1461-1474
2008
Bacillus licheniformis
Anikina, O.M.; Semashko, T.A.; Oksenoit, E.S.; Lysogorskaya, E.N.; Filippova, I.Y.
Native subtilisin Karlsberg and modified subtilisin 72 as effective catalysts of peptide bond formation in organic media
Russ. J. Bioorg. Chem.
32
116-121
2006
Bacillus subtilis, Bacillus licheniformis, Bacillus subtilis 72
-
Tulsidas, S.R.; Thangamani, S.; Ho, B.; Sivaraman, J.; Ding, J.L.
Crystallization of a nonclassical Kazal-type Carcinoscorpius rotundicauda serine protease inhibitor, CrSPI-1, complexed with subtilisin
Acta Crystallogr. Sect. F
65
533-535
2009
synthetic construct
Wong, W.; Kennan, R.M.; Rosado, C.J.; Rood, J.I.; Whisstock, J.C.; Porter, C.J.
Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus
Acta Crystallogr. Sect. F
66
289-293
2010
Dichelobacter nodosus, Dichelobacter nodosus (A5EVD0), Dichelobacter nodosus (A5EXI3), Dichelobacter nodosus (Q46547), Dichelobacter nodosus C305 (Q46547), Dichelobacter nodosus VCS1703A (A5EVD0), Dichelobacter nodosus VCS1703A (A5EXI3)
Sung, J.H.; Ahn, S.J.; Kim, N.Y.; Jeong, S.K.; Kim, J.K.; Chung, J.K.; Lee, H.H.
Purification, molecular cloning, and biochemical characterization of subtilisin JB1 from a newly isolated Bacillus subtilis JB1
Appl. Biochem. Biotechnol.
162
900-911
2010
Bacillus subtilis (B6VFQ8), Bacillus subtilis JB1 (B6VFQ8), Bacillus subtilis JB1
Salamin, K.; Sriranganadane, D.; Lechenne, B.; Jousson, O.; Monod, M.
Secretion of an endogenous subtilisin by Pichia pastoris strains GS115 and KM71
Appl. Environ. Microbiol.
76
4269-4276
2010
Komagataella pastoris
Kurata, A.; Uchimura, K.; Kobayashi, T.; Horikoshi, K.
Collagenolytic subtilisin-like protease from the deep-sea bacterium Alkalimonas collagenimarina AC40T
Appl. Microbiol. Biotechnol.
86
589-598
2010
Alkalimonas collagenimarina (D0VYQ9), Alkalimonas collagenimarina, Alkalimonas collagenimarina AC40T (D0VYQ9), Alkalimonas collagenimarina AC40T
Gallagher, T.; Ruan, B.; London, M.; Bryan, M.A.; Bryan, P.N.
Structure of a switchable subtilisin complexed with a substrate and with the activator azide
Biochemistry
48
10389-10394
2009
Bacillus amyloliquefaciens (P00782), Bacillus amyloliquefaciens
Takeuchi, Y.; Tanaka, S.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon
Biochemistry
48
10637-10643
2009
Thermococcus kodakarensis
Fasoli, E.; Ferrer, A.; Barletta, G.
Hydrogen/deuterium exchange study of subtilisin carlsberg during prolonged exposure to organic solvents
Biotechnol. Bioeng.
102
1025-1032
2009
Bacillus licheniformis
Pagan, M.; Sola, R.J.; Griebenow, K.
On the role of protein structural dynamics in the catalytic activity and thermostability of serine protease subtilisin Carlsberg
Biotechnol. Bioeng.
103
77-84
2009
synthetic construct
Tiwary, E.; Gupta, R.
Improved catalytic efficiency of a monomeric gamma-glutamyl transpeptidase from Bacillus licheniformis in presence of subtilisin
Biotechnol. Lett.
32
1137-1141
2010
synthetic construct
Li, J.; Yu, L.; Yang, J.; Dong, L.; Tian, B.; Yu, Z.; Liang, L.; Zhang, Y.; Wang, X.; Zhang, K.
New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina
BMC Evol. Biol.
10
68
2010
Arthrobotrys musiformis (A1YN94), Arthrobotrys yunnanensis (A1YN95), Arthrobotrys psychrophilus (A1YN96), Drechslerella coelobrocha (A1YN97), Dactylella shizishanna (A1YN98), Purpureocillium lilacinum (A8QMW6)
Bonifait, L.; de la Cruz Dominguez-Punaro, M.; Vaillancourt, K.; Bart, C.; Slater, J.; Frenette, M.; Gottschalk, M.; Grenier, D.
The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis
BMC Microbiol.
10
42
2010
Streptococcus suis, Streptococcus suis P1/7
Guang, C.; Phillips, R.D.
Purification, activity and sequence of angiotensin I converting enzyme inhibitory peptide from alcalase hydrolysate of peanut flour
J. Agric. Food Chem.
57
10102-10106
2009
synthetic construct
Zhou, F.; Xue, Z.; Wang, J.
Antihypertensive effects of silk fibroin hydrolysate by alcalase and purification of an ACE inhibitory dipeptide
J. Agric. Food Chem.
58
6735-6740
2010
Bacillus licheniformis
Lu, J.; Ren, D.F.; Xue, Y.L.; Sawano, Y.; Miyakawa, T.; Tanokura, M.
Isolation of an Antihypertensive Peptide from Alcalase Digest of Spirulina platensis
J. Agric. Food Chem.
58
7166-7171
2010
Bacillus licheniformis
Cianci, M.; Tomaszewski, B.; Helliwell, J.R.; Halling, P.J.
Crystallographic analysis of counterion effects on subtilisin enzymatic action in acetonitrile
J. Am. Chem. Soc.
132
2293-2300
2010
Bacillus licheniformis (P00780)
Pietrusewicz, E.; Sienczyk, M.; Oleksyszyn, J.
Novel diphenyl esters of peptidyl alpha-aminoalkylphosphonates as inhibitors of chymotrypsin and subtilisin
J. Enzyme Inhib. Med. Chem.
24
1229-1236
2009
Bacillus licheniformis
Bansal, V.; Delgado, Y.; Fasoli, E.; Ferrer, A.; Griebenow, K.; Secundo, F.; Barletta, G.L.
Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg
J. Mol. Catal. B
64
38-44
2010
Bacillus licheniformis
Yan, F.; Yan, J.; Sun, W.; Yao, L.; Wang, J.; Qi, Y.; Xu, H.
Thrombolytic effect of Subtilisin QK on carrageenan induced thrombosis model in mice
J. Thromb. Thrombolysis
28
444-448
2009
Bacillus subtilis, Bacillus subtilis QK02
Nieto, G.; Castillo, M.; Xiong, Y.L.; Alvarez, D.; Payne, F.A.; Garrido, M.D.
Antioxidant and emulsifying properties of alcalase-hydrolyzed potato proteins in meat emulsions with different fat concentrations
Meat Sci.
283
24-30
2009
Bacillus licheniformis
Foophow, T.; Tanaka, S.; Koga, Y.; Takano, K.; Kanaya, S.
Subtilisin-like serine protease from hyperthermophilic archaeon Thermococcus kodakaraensis with N- and C-terminal propeptides
Protein Eng. Des. Sel.
23
347-355
2010
Thermococcus kodakarensis
Almog, O.; Gonzalez, A.; Godin, N.; De Leeuw, M.; Mekel, M.; Klein, D.; Braun, S.; Shoham, G.; Walter, R.
The crystal structures of the psychrophilic subtilisin S41 and the mesophilic subtilisin Sph reveal the same calcium-loaded state
Proteins
74
489-496
2009
Lysinibacillus sphaericus (Q9S3L6), Lysinibacillus sphaericus
Clinton, B.; Warden, A.; Haboury, S.; Easton, C.; Kotsonis, S.; Taylor, M.; Oakeshott, J.; Russell, R.; Scott, C.
Bacterial degradation of strobilurin fungicides: A role for a promiscuous methyl esterase activity of the subtilisin proteases?
Biocatal. Biotransform.
29
119-129
2011
Bacillus sp. (in: Bacteria)
-
Rai, S.; Mukherjee, A.
Statistical optimization of production, purification and industrial application of a laundry detergent and organic solvent-stable subtilisin-like serine protease (Alzwiprase) from Bacillus subtilis DM-04
Biochem. Eng. J.
48
173-180
2010
Bacillus subtilis
-
Vartapetian, A.B.; Tuzhikov, A.I.; Chichkova, N.V.; Taliansky, M.; Wolpert, T.J.
A plant alternative to animal caspases: subtilisin-like proteases
Cell Death Differ.
18
1289-1297
2011
Arabidopsis thaliana, Avena sativa, Glycine max, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa
Falcioni, F.; Housden, H.R.; Ling, Z.; Shimizu, S.; Walker, A.J.; Bruce, N.C.
Soluble, folded and active subtilisin in a protic ionic liquid
Chem. Commun. (Camb. )
46
749-751
2010
Bacillus licheniformis
Kim, J.; Kim, J.H.; Choi, K.H.; Kim, J.H.; Song, Y.S.; Cha, J.
Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis
J. Agric. Food Chem.
59
8675-8682
2011
Bacillus amyloliquefaciens
Swenerton, R.K.; Knudsen, G.M.; Sajid, M.; Kelly, B.L.; McKerrow, J.H.
Leishmania subtilisin is a maturase for the trypanothione reductase system and contributes to disease pathology
J. Biol. Chem.
285
31120-31129
2010
Leishmania donovani
Wong, W.; Wijeyewickrema, L.C.; Kennan, R.M.; Reeve, S.B.; Steer, D.L.; Reboul, C.; Smith, A.I.; Pike, R.N.; Rood, J.I.; Whisstock, J.C.; Porter, C.J.
The S1 pocket of a bacterial derived subtilisin-like protease underpins effective tissue destruction
J. Biol. Chem.
286
42180-42187
2011
Dichelobacter nodosus
Boeriu, C.; Frissen, A.; Boer, E.; Van Kekem, K.; Van Zoelen, D.; Eggen, I.
Optimized enzymatic synthesis of C-terminal peptide amides using subtilisin A from Bacillus licheniformis
J. Mol. Catal. B
66
33-42
2010
Bacillus licheniformis
-
Tiberti, M.; Papaleo, E.
Dynamic properties of extremophilic subtilisin-like serine-proteases
J. Struct. Biol.
174
69-83
2011
Thermoactinomyces vulgaris, Vibrio sp.
Ghasemi, Y.; Dabbagh, F.; Ghasemian, A.
Cloning of a fibrinolytic enzyme (Subtilisin) gene from Bacillus subtilis in Escherichia coli
Mol. Biotechnol.
52
1-7
2012
Bacillus subtilis (Q84F18), Bacillus subtilis
Shi, C.; Miller, J.D.
Characterization of the 41kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor
Mol. Immunol.
48
1827-1834
2011
Aspergillus versicolor
Addae, J.I.; Stone, T.W.
The serine protease subtilisin suppresses epileptiform activity in rat hippocampal slices and neocortex in vivo
Neuroscience
199
64-73
2011
Bacillus licheniformis
Hernandez-Romano, P.; Hernandez, R.; Arroyo, R.; Alderete, J.F.; Lopez-Villasenor, I.
Identification and characterization of a surface-associated, subtilisin-like serine protease in Trichomonas vaginalis
Parasitology
137
1621-1635
2010
Trichomonas vaginalis (D3G8F6), Trichomonas vaginalis
Kennan, R.; Wong, W.; Dhungyel, O.; Han, X.; Wong, D.; Parker, D.; Rosado, C.; Law, R.; McGowan, S.; Reeve, S.; Levina, V.; Powers, G.; Pike, R.; Bottomley, S.; Ian Smith, A.; Marsh, I.; Whittington, R.; Whisstock, J.; Porter, C.; Rood, J.
The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates
PLoS Pathog.
6
e1001210
2010
Dichelobacter nodosus
Gamble, M.; Kuenze, G.; Dodson, E.J.; Wilson, K.S.; Jones, D.D.
Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism
Proc. Natl. Acad. Sci. USA
108
3536-3541
2011
Alkalihalobacillus clausii (D0AB41)
Martinez, R.; Schwaneberg, U.; Roccatano, D.
Temperature effects on structure and dynamics of the psychrophilic protease subtilisin S41 and its thermostable mutants in solution
Protein Eng. Des. Sel.
24
533-544
2011
Bacillus subtilis (Q45681)
Vevodova, J.; Gamble, M.; Kuenze, G.; Ariza, A.; Dodson, E.; Jones, D.D.; Wilson, K.S.
Crystal structure of an intracellular subtilisin reveals novel structural features unique to this subtilisin family
Structure
18
744-755
2010
Alkalihalobacillus clausii (D0AB41)
Bonifait, L.; Vaillancourt, K.; Gottschalk, M.; Frenette, M.; Grenier, D.
Purification and characterization of the subtilisin-like protease of Streptococcus suis that contributes to its virulence
Vet. Microbiol.
148
333-340
2011
Streptococcus suis
Rüdiger, A.; Rüdiger, M.; Weber, K.; Schomburg, D.
Characterization of post-translational modifications of brain tubulin by matrix-assisted laser desorption/ionization mass spectrometry: direct one-step analysis of a limited subtilisin digest
Anal. Biochem.
224
532-537
1995
Bacillus licheniformis
Uehara, R.; Takeuchi, Y.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis
Biochemistry
51
5369-5378
2012
Thermococcus kodakarensis (Q5JIZ5), Thermococcus kodakarensis
Juntunen, K.; Mäkinen, S.; Isoniemi, S.; Valtakari, L.; Pelzer, A.; Jänis, J.; Paloheimo, M.
A new subtilase-like protease deriving from Fusarium equiseti with high potential for industrial applications
Appl. Biochem. Biotechnol.
177
407-430
2015
Fusarium equiseti, Fusarium equiseti CBS119568
Jakob, F.; Martinez, R.; Mandawe, J.; Hellmuth, H.; Siegert, P.; Maurer, K.H.; Schwaneberg, U.
Surface charge engineering of a Bacillus gibsonii subtilisin protease
Appl. Microbiol. Biotechnol.
97
6793-6802
2013
Alkalihalobacillus gibsonii (GN111900.1), Alkalihalobacillus gibsonii, Alkalihalobacillus gibsonii DSM 14391 (GN111900.1)
Petrillo, T.; ODonohoe, C.A.; Howe, N.; Malthouse, J.P.
Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin
Biochemistry
51
6164-6170
2012
Bacillus sp. (in: Bacteria)
Fuchita, N.; Arita, S.; Ikuta, J.; Miura, M.; Shimomura, K.; Motoshima, H.; Watanabe, K.
Gly or Ala substitutions for Pro210Thr211Asn212 at the beta8-beta9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability
Biochim. Biophys. Acta
1824
620-626
2012
Bacillus licheniformis (P00780)
Li, Z.; Roccatano, D.; Lorenz, M.; Schwaneberg, U.
Directed evolution of subtilisin E into a highly active and guanidinium chloride- and sodium dodecylsulfate-tolerant protease
ChemBioChem
13
691-699
2012
Bacillus subtilis (P04189)
Gamble, M.; Kuenze, G.; Brancale, A.; Wilson, K.S.; Jones, D.D.
The role of substrate specificity and metal binding in defining the activity and structure of an intracellular subtilisin
FEBS open bio
2
209-215
2012
Alkalihalobacillus clausii (D0AB41), Alkalihalobacillus clausii
Acevedo, J.P.; Rodriguez, V.; Saavedra, M.; Munoz, M.; Salazar, O.; Asenjo, J.A.; Andrews, B.A.
Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease
J. Appl. Microbiol.
114
352-363
2013
Euphausia superba
Chalfoun, N.R.; Grellet-Bournonville, C.F.; Martinez-Zamora, M.G.; Diaz-Perales, A.; Castagnaro, A.P.; Diaz-Ricci, J.C.
Purification and characterization of AsES protein: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor
J. Biol. Chem.
288
14098-14113
2013
Sarocladium strictum
Despotovic, D.; Vojcic, L.; Blanusa, M.; Maurer, K.H.; Zacharias, M.; Bocola, M.; Martinez, R.; Schwaneberg, U.
Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg
J. Biotechnol.
167
279-286
2013
Bacillus licheniformis (P00780)
Li, Z.; Roccatano, D.; Lorenz, M.; Martinez, R.; Schwaneberg, U.
Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate
J. Biotechnol.
169
87-94
2014
Bacillus subtilis (P04189)
Abdizadeh, H.; Guven, G.; Atilgan, A.R.; Atilgan, C.
Perturbation response scanning specifies key regions in subtilisin serine protease for both function and stability
J. Enzyme Inhib. Med. Chem.
30
867-873
2015
Bacillus licheniformis
Martinez, D.E.; Borniego, M.L.; Battchikova, N.; Aro, E.M.; Tyystjaervi, E.; Guiamet, J.J.
SASP, a senescence-associated subtilisin protease, is involved in reproductive development and determination of silique number in Arabidopsis
J. Exp. Bot.
66
161-174
2015
Arabidopsis thaliana (Q0WWH7), Arabidopsis thaliana
Fan, H.; Liu, Z.; Zhang, R.; Wang, N.; Dou, K.; Mijiti, G.; Diao, G.; Wang, Z.
Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma harzianum
J. Microbiol.
52
129-138
2014
Trichoderma harzianum (S5S2B8), Trichoderma harzianum, Trichoderma harzianum ACCC30371 (S5S2B8), Trichoderma harzianum ACCC30371
Falus, P.; Cerioli, L.; Bajnoczi, G.; Boros, Z.; Weiser, D.; Nagy, J.; Tessaro, D.; Servi, S.; Poppe, L.
A continuous-flow cascade reactor system for subtilisin A-catalyzed dynamic kinetic resolution of N-tert-butyloxycarbonylphenylalanine ethyl thioester with benzylamine
Adv. Synth. Catal.
358
1608-1617
2016
Bacillus licheniformis
-
Juntunen, K.; Maekinen, S.; Isoniemi, S.; Valtakari, L.; Pelzer, A.; Jaenis, J.; Paloheimo, M.
A new subtilase-like protease deriving from Fusarium equiseti with high potential for industrial applications
Appl. Biochem. Biotechnol.
177
407-430
2015
Fusarium equiseti, Fusarium equiseti CBS 119568
Tang, H.; Zhang, J.; Shi, K.; Aihara, H.; Du, G.
Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis
Biochem. Biophys. Res. Commun.
512
623-628
2019
Bacillus subtilis, Bacillus subtilis JN-S7
Oskarsson, K.R.; Kristjansson, M.M.
Improved expression, purification and characterization of VPR, a cold active subtilisin-like serine proteinase and the effects of calcium on expression and stability
Biochim. Biophys. Acta
1867
152-162
2019
Bacillus subtilis (P29141), Bacillus subtilis 168 (P29141)
Dorau, R.; Goerbe, T.; Svedendahl Humble, M.
Improved enantioselectivity of subtilisin Carlsberg towards secondary alcohols by protein engineering
ChemBioChem
19
338-346
2018
Bacillus licheniformis (P00780)
Li, D.H.; Xi, H.; Yu, X.B.; Cai, Y.P.
Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana
Genet. Mol. Res.
14
16535-16545
2015
Arabidopsis thaliana (Q9FHA4), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (Q9FHA4)
Howell, M.; Dumitrescu, D.G.; Blankenship, L.R.; Herkert, D.; Hatzios, S.K.
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
J. Biol. Chem.
294
9888-9900
2019
Vibrio cholerae serotype O1 (Q9KVI8), Vibrio cholerae serotype O1 C6706 (Q9KVI8), Vibrio cholerae serotype O1 El Tor Inaba N16961 (Q9KVI8), Vibrio cholerae serotype O1 ATCC 39315 (Q9KVI8)
Qoura, F.; Kassab, E.; Reisse, S.; Antranikian, G.; Brueck, T.
Characterization of a new, recombinant thermo-active subtilisin-like serine protease derived from Shewanella arctica
J. Mol. Catal. B
116
16-23
2015
Shewanella frigidimarina (A0A0B6XK99), Shewanella frigidimarina DSM 16509 (A0A0B6XK99), Shewanella frigidimarina JCM 14208 (A0A0B6XK99)
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Armistead, J.S.; Jennison, C.; O'Neill, M.T.; Lopaticki, S.; Liehl, P.; Hanson, K.K.; Annoura, T.; Rajasekaran, P.; Erickson, S.M.; Tonkin, C.J.; Khan, S.M.; Mota, M.M.; Boddey, J.A.
Plasmodium falciparum subtilisin-like ookinete protein SOPT plays an important and conserved role during ookinete infection of the Anopheles stephensi midgut
Mol. Microbiol.
109
458-473
2018
Plasmodium berghei (A0A509AKI1), Plasmodium berghei, Plasmodium falciparum (Q8I429), Plasmodium falciparum, Plasmodium berghei ANKA (A0A509AKI1)
Kim, K.; Lee, E.; Kim, C.; Park, J.; Kim, M.J.
Ionic-surfactant-coated subtilisin activity, enantioselectivity, and application to dynamic kinetic resolution of secondary alcohols
Org. Biomol. Chem.
15
8836-8844
2017
Bacillus licheniformis
Price, M.A.; Cruz, R.; Baxter, S.; Escalettes, F.; Rosser, S.J.
CRISPR-Cas9 in situ engineering of subtilisin E in Bacillus subtilis
PLoS ONE
14
e0210121
2019
Bacillus subtilis (P04189), Bacillus subtilis, Bacillus subtilis 168 (P04189)
Zhou, K.; Dong, Y.; Zheng, H.; Chen, B.; Mao, R.; Zhou, L.; Wang, Y.
Expression, fermentation, purification and lyophilisation of recombinant subtilisin QK in Pichia pastoris
Process Biochem.
54
1-8
2017
Bacillus subtilis (Q7WVA6), Bacillus subtilis QK02 (Q7WVA6)
-
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