Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | - |
Akanthomyces muscarius | |
chymostatin | - |
Lecanicillium longisporum | |
additional information | not inhibited by 1,10 phenanthroline | Akanthomyces muscarius | |
additional information | not inhibited by 1,10 phenanthroline | Lecanicillium longisporum | |
nitrogen | together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h | Akanthomyces muscarius | |
nitrogen | absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h | Lecanicillium longisporum | |
sucrose | together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h | Akanthomyces muscarius | |
sucrose | absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h | Lecanicillium longisporum | |
turkey egg white inhibitor | - |
Akanthomyces muscarius | |
turkey egg white inhibitor | - |
Lecanicillium longisporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Akanthomyces muscarius | - |
KV01 and KV54 | - |
Lecanicillium longisporum | - |
KV71, KV22 and KV42 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
Bovine serum albumin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
chitin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
chitin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
cuticle + H2O | Pr1-like activity on host and non-host cuticle. In KV01 cultures, at 16 h, similar Pr1-like activity on both aphid and locust cuticle | Akanthomyces muscarius | ? | - |
? | |
cuticle + H2O | Pr1-like activity on host and non-host cuticle. In KV71 cultures, at 16 and 24 h, similar Pr1-like activity on both aphid and locust cuticle. Filtrates from KV71 and KV42 cultures, that contain cocktails of cuticle-degrading enzymes, hydrolyse 3-4fold more protein from aphid than locust cuticle | Lecanicillium longisporum | ? | - |
? | |
KOH chitin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
KOH chitin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
N-acetylglucosamine + H2O | N-acetylglucosamine has little effect on subtilisin production by KV01 (11% of that on cuticle) | Akanthomyces muscarius | ? | - |
? | |
N-acetylglucosamine + H2O | restricted feeding of N-acetylglucosamine (the monomer of chitin) to biomass of KV71 induces protease to 61% of that on cuticle | Lecanicillium longisporum | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Akanthomyces muscarius | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
subtilisin Pr1-like protease | - |
Lecanicillium longisporum |
subtilisin Pr1-like protease | - |
Akanthomyces muscarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Lecanicillium longisporum |
7 | 10 | - |
Akanthomyces muscarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8 |
Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.19 |
Lecanicillium longisporum | isoelectric focusing, KV71 | - |
8.62 |
Lecanicillium longisporum | isoelectric focusing, KV71 | - |
8.83 |
Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.92 |
Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.98 |
Lecanicillium longisporum | isoelectric focusing, KV71 | - |
9.1 |
Lecanicillium longisporum | isoelectric focusing, KV71 and KV42 | - |
9.47 |
Akanthomyces muscarius | KV01 | - |
9.47 |