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Literature summary for 3.4.21.62 extracted from

  • Bye, N.J.; Charnley, A.K.
    Regulation of cuticle-degrading subtilisin proteases from the entomopathogenic fungi, Lecanicillium spp: implications for host specificity (2008), Arch. Microbiol., 189, 81-92.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
chymostatin
-
Akanthomyces muscarius
chymostatin
-
Lecanicillium longisporum
additional information not inhibited by 1,10 phenanthroline Akanthomyces muscarius
additional information not inhibited by 1,10 phenanthroline Lecanicillium longisporum
nitrogen together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h Akanthomyces muscarius
nitrogen absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h Lecanicillium longisporum
sucrose together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h Akanthomyces muscarius
sucrose absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h Lecanicillium longisporum
turkey egg white inhibitor
-
Akanthomyces muscarius
turkey egg white inhibitor
-
Lecanicillium longisporum

Organism

Organism UniProt Comment Textmining
Akanthomyces muscarius
-
KV01 and KV54
-
Lecanicillium longisporum
-
KV71, KV22 and KV42
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O
-
Lecanicillium longisporum ?
-
?
Bovine serum albumin + H2O
-
Akanthomyces muscarius ?
-
?
chitin + H2O
-
Lecanicillium longisporum ?
-
?
chitin + H2O
-
Akanthomyces muscarius ?
-
?
cuticle + H2O Pr1-like activity on host and non-host cuticle. In KV01 cultures, at 16 h, similar Pr1-like activity on both aphid and locust cuticle Akanthomyces muscarius ?
-
?
cuticle + H2O Pr1-like activity on host and non-host cuticle. In KV71 cultures, at 16 and 24 h, similar Pr1-like activity on both aphid and locust cuticle. Filtrates from KV71 and KV42 cultures, that contain cocktails of cuticle-degrading enzymes, hydrolyse 3-4fold more protein from aphid than locust cuticle Lecanicillium longisporum ?
-
?
KOH chitin + H2O
-
Lecanicillium longisporum ?
-
?
KOH chitin + H2O
-
Akanthomyces muscarius ?
-
?
N-acetylglucosamine + H2O N-acetylglucosamine has little effect on subtilisin production by KV01 (11% of that on cuticle) Akanthomyces muscarius ?
-
?
N-acetylglucosamine + H2O restricted feeding of N-acetylglucosamine (the monomer of chitin) to biomass of KV71 induces protease to 61% of that on cuticle Lecanicillium longisporum ?
-
?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
Lecanicillium longisporum ?
-
?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
Akanthomyces muscarius ?
-
?

Synonyms

Synonyms Comment Organism
subtilisin Pr1-like protease
-
Lecanicillium longisporum
subtilisin Pr1-like protease
-
Akanthomyces muscarius

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Lecanicillium longisporum
7 10
-
Akanthomyces muscarius

pI Value

Organism Comment pI Value Maximum pI Value
Lecanicillium longisporum isoelectric focusing, KV42
-
8
Lecanicillium longisporum isoelectric focusing, KV42
-
8.19
Lecanicillium longisporum isoelectric focusing, KV71
-
8.62
Lecanicillium longisporum isoelectric focusing, KV71
-
8.83
Lecanicillium longisporum isoelectric focusing, KV42
-
8.92
Lecanicillium longisporum isoelectric focusing, KV42
-
8.98
Lecanicillium longisporum isoelectric focusing, KV71
-
9.1
Lecanicillium longisporum isoelectric focusing, KV71 and KV42
-
9.47
Akanthomyces muscarius KV01
-
9.47