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Literature summary for 3.4.21.62 extracted from
- Pro-subtilisin E: purification and characterization of its autoprocessing to active subtilisin E in vitro (1990), Mol. Microbiol., 4, 295-304.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subtilisin E produced in Escherichia coli | Bacillus subtilis |
| subtilisin E produced in Escherichia coli | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
subtilisin E | - |
| Bacillus sp. (in: Bacteria) | - |
subtilisin E wild-type and mutant D32N produced in Escherichia coli | - |
| Bacillus subtilis | - |
Bacillus subtilis enzyme, pro-subtilisin E produced in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of pro-subtilisin E wild-type and mutant (Asp32 to Asn) and intramolecular autoprocessing to active subtilisin E in vitro | Bacillus subtilis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| denatured subtilisin E cannot be refolded to form active subtilisin E under the conditions used for processing (of pro-subtilisin to yield active subtilisin) | Bacillus subtilis |
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