Crystallization (Comment) | Organism |
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by the hanging drop vapor-diffusion method, at 0.8 A resolution. Crystals belong to the monoclinic space group P21. Unit cell parameters are a = 46.09 A, b = 62.67 A, c = 84.87 A, and beta = 95.5°, indicating two molecules of Sph in the asymmetric unit. The crystal structures of the psychrophilic Bacillus TA41 subtilisin S41 and the mesophilic subtilisin Sph are nearly identical with the same calcium-loaded state in that five calcium ions are bound to each protein molecule | Lysinibacillus sphaericus |
by the hanging drop vapor-diffusion method, at 1.4 A resolution. Crystals belong to either the tetragonal space group P41212 or P43212. Unit cell dimensions are a = b = 61 A and c = 174.77 A , indicating one monomer in the asymmetric unit. The crystal structures of the psychrophilic subtilisin S41 and the mesophilic Bacillus sphaericus subtilisin Sph are nearly identical with the same calcium-loaded state in that five calcium ions are bound to each protein molecule | Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
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Ca2+ | calcium-loaded state of five ions bound to each of the two subtilisin molecules. Three of the binding sites have two side chains of an acidic residue coordinating the calcium ion, whereas the other two binding sites have either a main-chain carbonyl, or only one acidic residue side chain coordinating the calcium ion | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
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Lysinibacillus sphaericus | Q9S3L6 | - |
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Synonyms | Comment | Organism |
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Subtilisin S41 | - |
Lysinibacillus sphaericus |
subtilisin Sph | - |
Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
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additional information | - |
the high structural similarity between Bacillus sphaericus Sph and S41 and their identical calcium-binding mode, indicates that the calcium-loaded state is not responsible for the cold adaptation of S41 | Lysinibacillus sphaericus |